Construct: ORF ccsbBroadEn_10518

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007688.1_s300c1, BRDN0000385938
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TMEM185B (79134)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 79134 TMEM185B transmembrane protein 185B NM_024121.3 99.9% 100% 111A>G
2 human 84548 TMEM185A transmembrane protein 185A NM_032508.4 80.4% 88.5% (many diffs)
3 human 84548 TMEM185A transmembrane protein 185A NM_001174092.2 67.5% 73.7% (many diffs)
4 human 84548 TMEM185A transmembrane protein 185A NM_001282302.2 39.3% 44.7% (many diffs)
5 mouse 226351 Tmem185b transmembrane protein 185B NM_146103.2 88.8% 96% (many diffs)
6 mouse 236848 Tmem185a transmembrane protein 185A XM_006527981.2 80.9% 88.8% (many diffs)
7 mouse 236848 Tmem185a transmembrane protein 185A NM_001033328.2 74.3% 81.4% (many diffs)
8 mouse 236848 Tmem185a transmembrane protein 185A XM_017318471.1 62.2% 68% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1116
ORF length:
1050
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGAA CCCCAGGGGC CTGTTCCAGG ACTTCAACCC CAGTAAGTTT CTCATCTACA
121CCTGCCTGCT GCTCTTCTCG GTGCTGCTGC CCCTCCGCCT GGACGGCATC ATCCAGTGGA
181GCTACTGGGC CGTCTTTGCC CCCATATGGC TGTGGAAGCT TCTAGTCGTC GCAGGCGCCT
241CCGTGGGCGC GGGCGTTTGG GCCCGCAACC CTCGCTACCG CACCGAGGGA GAGGCCTGTG
301TGGAGTTCAA AGCCATGCTG ATCGCTGTGG GCATCCACCT GCTGCTGCTC ATGTTCGAAG
361TCCTGGTCTG CGACAGGGTG GAGAGGGGCA CCCACTTCTG GCTGCTGGTC TTCATGCCTC
421TCTTCTTCGT GTCCCCCGTG TCCGTGGCTG CCTGCGTCTG GGGCTTTCGA CACGATAGGT
481CGCTGGAGCT GGAGATCCTG TGCTCGGTCA ACATCCTGCA GTTCATCTTC ATCGCCCTAA
541AGCTGGACAG GATTATTCAC TGGCCGTGGC TGGTGGTGTT TGTGCCCCTG TGGATCCTCA
601TGTCGTTCCT TTGCCTGGTC GTCCTCTATT ACATCGTCTG GTCCCTCCTG TTCCTGCGGT
661CCCTGGATGT GGTTGCCGAG CAGCGGAGAA CACACGTGAC CATGGCTATC AGTTGGATAA
721CGATTGTCGT GCCTCTGCTC ACTTTTGAGG TCCTGCTGGT TCACAGATTG GATGGCCACA
781ATACATTCTC CTACGTCTCC ATATTTGTCC CCCTTTGGCT TTCCTTACTA ACTTTAATGG
841CCACAACATT TAGGCGAAAG GGGGGCAATC ATTGGTGGTT TGGCATTCGC AGAGACTTCT
901GTCAGTTTCT GCTTGAAATT TTCCCATTTT TAAGAGAATA TGGGAACATT TCATATGATC
961TCCATCACGA AGATAGTGAA GATGCTGAAG AAACATCAGT TCCAGAAGCT CCGAAAATTG
1021CTCCAATATT TGGAAAGAAG GCCAGAGTAG TTATAACCCA GAGCCCTGGG AAATACGTTC
1081CCCCCCCTCC CAAGTTAAAT ATTGATATGC CAGATTACCC AACTTTCTTG TACAAAGTtg
1141gcattataag aaagcattgc ttatcaattt gttgcaacga ac

Download FASTA (ORF) (Full)