Construct: ORF ccsbBroadEn_10680
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015282.1_s300c1, BRDN0000385054
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- APBB2 (323)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 323 | APBB2 | amyloid beta precursor prot... | NM_001166051.2 | 63.3% | 63.3% | 0_1ins360;84_86delAGT |
2 | human | 323 | APBB2 | amyloid beta precursor prot... | NM_001166052.2 | 63.3% | 63.3% | 0_1ins360;84_86delAGT |
3 | human | 323 | APBB2 | amyloid beta precursor prot... | NM_001166053.1 | 63.3% | 63.3% | 0_1ins360;84_86delAGT |
4 | human | 323 | APBB2 | amyloid beta precursor prot... | NM_001166054.1 | 63.3% | 63.3% | 0_1ins360;84_86delAGT |
5 | human | 323 | APBB2 | amyloid beta precursor prot... | XM_017008158.2 | 45.6% | 45.6% | 1_1173del;1617_1619delAGT |
6 | human | 323 | APBB2 | amyloid beta precursor prot... | XM_017008156.2 | 45.5% | 45.5% | 1_1173del;1415_1420delGAGAGA;1623_1625delAGT |
7 | human | 323 | APBB2 | amyloid beta precursor prot... | XM_017008157.2 | 45.5% | 45.5% | 1_1173del;1415_1420delGAGAGA;1623_1625delAGT |
8 | human | 323 | APBB2 | amyloid beta precursor prot... | NM_173075.5 | 44.7% | 44.7% | 1_1221del |
9 | human | 323 | APBB2 | amyloid beta precursor prot... | NM_001330656.2 | 44.6% | 44.6% | 1_1221del;1665_1667delAGT |
10 | human | 323 | APBB2 | amyloid beta precursor prot... | NM_001330658.2 | 44.6% | 44.6% | 1_1221del;1665_1667delAGT |
11 | human | 323 | APBB2 | amyloid beta precursor prot... | XM_011513687.3 | 44.5% | 44.5% | 1_1224del;1668_1670delAGT |
12 | human | 323 | APBB2 | amyloid beta precursor prot... | XM_017008154.2 | 44.5% | 44.5% | 1_1224del;1668_1670delAGT |
13 | human | 323 | APBB2 | amyloid beta precursor prot... | XM_006714012.4 | 44.5% | 44.5% | 1_1221del;1463_1468delGAGAGA;1671_1673delAGT |
14 | human | 323 | APBB2 | amyloid beta precursor prot... | XM_017008152.2 | 44.5% | 44.5% | 1_1221del;1463_1468delGAGAGA;1671_1673delAGT |
15 | human | 323 | APBB2 | amyloid beta precursor prot... | XM_006714011.4 | 44.4% | 44.4% | 1_1224del;1466_1471delGAGAGA;1674_1676delAGT |
16 | human | 323 | APBB2 | amyloid beta precursor prot... | XM_017008151.2 | 44.4% | 44.4% | 1_1224del;1466_1471delGAGAGA;1674_1676delAGT |
17 | human | 323 | APBB2 | amyloid beta precursor prot... | XM_017008149.2 | 44.2% | 44.2% | 1_1233del;1475_1480delGAGAGA;1683_1685delAGT |
18 | human | 323 | APBB2 | amyloid beta precursor prot... | XM_017008146.2 | 44.2% | 44.2% | 1_1236del;1478_1483delGAGAGA;1686_1688delAGT |
19 | human | 323 | APBB2 | amyloid beta precursor prot... | XM_017008147.2 | 44.2% | 44.2% | 1_1236del;1478_1483delGAGAGA;1686_1688delAGT |
20 | human | 323 | APBB2 | amyloid beta precursor prot... | XM_017008148.2 | 44.2% | 44.2% | 1_1236del;1478_1483delGAGAGA;1686_1688delAGT |
21 | human | 323 | APBB2 | amyloid beta precursor prot... | NM_001166050.2 | 43.4% | 43.4% | 1_1284del;1728_1730delAGT |
22 | human | 323 | APBB2 | amyloid beta precursor prot... | NM_004307.2 | 43.3% | 43.3% | 1_1287del;1731_1733delAGT |
23 | human | 323 | APBB2 | amyloid beta precursor prot... | XM_017008145.2 | 43.3% | 43.3% | 1_1287del;1731_1733delAGT |
24 | human | 323 | APBB2 | amyloid beta precursor prot... | XM_006714010.4 | 43.2% | 43.2% | 1_1284del;1526_1531delGAGAGA;1734_1736delAGT |
25 | human | 323 | APBB2 | amyloid beta precursor prot... | XM_017008143.2 | 43.2% | 43.2% | 1_1284del;1526_1531delGAGAGA;1734_1736delAGT |
26 | human | 323 | APBB2 | amyloid beta precursor prot... | XM_017008144.2 | 43.2% | 43.2% | 1_1284del;1526_1531delGAGAGA;1734_1736delAGT |
27 | human | 323 | APBB2 | amyloid beta precursor prot... | XM_006714005.4 | 43.2% | 43.2% | 1_1287del;1529_1534delGAGAGA;1737_1739delAGT |
28 | human | 323 | APBB2 | amyloid beta precursor prot... | XM_006714007.4 | 43.2% | 43.2% | 1_1287del;1529_1534delGAGAGA;1737_1739delAGT |
29 | human | 323 | APBB2 | amyloid beta precursor prot... | XM_006714008.4 | 43.2% | 43.2% | 1_1287del;1529_1534delGAGAGA;1737_1739delAGT |
30 | human | 323 | APBB2 | amyloid beta precursor prot... | XM_006714009.4 | 43.2% | 43.2% | 1_1287del;1529_1534delGAGAGA;1737_1739delAGT |
31 | human | 323 | APBB2 | amyloid beta precursor prot... | XM_011513686.3 | 43.2% | 43.2% | 1_1287del;1529_1534delGAGAGA;1737_1739delAGT |
32 | mouse | 11787 | Apbb2 | amyloid beta (A4) precursor... | XM_017320622.1 | 59.9% | 65% | (many diffs) |
33 | mouse | 11787 | Apbb2 | amyloid beta (A4) precursor... | NM_001201416.1 | 56.3% | 60.3% | (many diffs) |
34 | mouse | 11787 | Apbb2 | amyloid beta (A4) precursor... | XM_006503696.3 | 56.3% | 60.3% | (many diffs) |
35 | mouse | 11787 | Apbb2 | amyloid beta (A4) precursor... | XM_006503697.3 | 56.3% | 60.3% | (many diffs) |
36 | mouse | 11787 | Apbb2 | amyloid beta (A4) precursor... | NM_001310626.1 | 39.7% | 43% | (many diffs) |
37 | mouse | 11787 | Apbb2 | amyloid beta (A4) precursor... | NM_001201415.1 | 39.6% | 43% | (many diffs) |
38 | mouse | 11787 | Apbb2 | amyloid beta (A4) precursor... | NM_001201414.1 | 39.5% | 42.9% | (many diffs) |
39 | mouse | 11787 | Apbb2 | amyloid beta (A4) precursor... | XM_006503694.3 | 39.5% | 42.9% | (many diffs) |
40 | mouse | 11787 | Apbb2 | amyloid beta (A4) precursor... | XM_006503693.3 | 39.4% | 42.8% | (many diffs) |
41 | mouse | 11787 | Apbb2 | amyloid beta (A4) precursor... | NM_001201413.1 | 38.5% | 41.8% | (many diffs) |
42 | mouse | 11787 | Apbb2 | amyloid beta (A4) precursor... | XM_006503692.3 | 38.5% | 41.8% | (many diffs) |
43 | mouse | 11787 | Apbb2 | amyloid beta (A4) precursor... | XM_017320620.1 | 38.4% | 41.7% | (many diffs) |
44 | mouse | 11787 | Apbb2 | amyloid beta (A4) precursor... | XM_017320621.1 | 38.4% | 41.7% | (many diffs) |
45 | mouse | 11787 | Apbb2 | amyloid beta (A4) precursor... | NM_009686.2 | 38.4% | 41.7% | (many diffs) |
46 | mouse | 11787 | Apbb2 | amyloid beta (A4) precursor... | XM_011240693.2 | 38.4% | 41.7% | (many diffs) |
47 | mouse | 11787 | Apbb2 | amyloid beta (A4) precursor... | XM_006503688.3 | 38.3% | 41.6% | (many diffs) |
48 | mouse | 11787 | Apbb2 | amyloid beta (A4) precursor... | XM_006503690.3 | 38.3% | 41.6% | (many diffs) |
49 | mouse | 11787 | Apbb2 | amyloid beta (A4) precursor... | XM_006503691.3 | 38.3% | 41.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1053
- ORF length:
- 987
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGC AGAAGAGGAC CTCGCCCCCG GTAAAAGTAG TGTTGCGGTC AACAACTGCA 121 TCAGGCAACT TTCCTACTGC AAAAATGACA TCCGAGACAC AGTCGGGATT TGGGGAGAGG 181 GGAAAGACAT GTACCTGATC CTGGAGAATG ACATGCTCAG CCTGGTGGAC CCCATGGACC 241 GCAGCGTGCT GCACTCGCAG CCCATCGTCA GCATCCGCGT GTGGGGCGTG GGCCGCGACA 301 ATGGCCGGGA TTTTGCTTAT GTAGCAAGAG ATAAAGATAC AAGAATTTTG AAATGTCATG 361 TATTTCGATG TGACACACCA GCAAAAGCCA TTGCCACAAG TCTCCACGAG ATCTGCTCCA 421 AGATTATGGC TGAACGGAAG AATGCCAAAG CGCTGGCCTG CAGCTCCTTA CAGGAAAGGG 481 CCAATGTGAA CCTCGATGTC CCTTTGCAAG ATTTTCCAAC ACCAAAGACT GAGCTGGTCC 541 AGAAGTTCCA CGTGCAGTAC TTGGGCATGT TACCTGTAGA CAAACCAGTC GGAATGGATA 601 TTTTGAACAG TGCCATAGAA AATCTTATGA CCTCATCCAA CAAGGAGGAC TGGCTGTCAG 661 TGAACATGAA CGTGGCTGAT GCCACTGTGA CTGTCATCAG TGAAAAGAAT GAAGAGGAAG 721 TCTTAGTGGA ATGTCGTGTG CGATTCCTGT CCTTCATGGG TGTTGGGAAG GACGTCCACA 781 CATTTGCCTT CATCATGGAC ACGGGGAACC AGCGCTTTGA GTGCCACGTT TTCTGGTGCG 841 AGCCTAATGC TGGTAACGTG TCTGAGGCGG TGCAGGCCGC CTGCATGTTA CGATATCAGA 901 AGTGCTTGGT AGCCAGGCCG CCTTCTCAGA AAGTTCGACC ACCTCCACCG CCAGCAGATT 961 CAGTAACCAG AAGAGTCACA ACCAATGTAA AACGAGGGGT CTTATCCCTC ATTGACACTT 1021 TGAAACAGAA ACGCCCTGTC ACCGAAATGC CATACCCAAC TTTCTTGTAC AAAGTtggca 1081 ttataagaaa gcattgctta tcaatttgtt gcaacgaac