Transcript: Human NM_001166054.1

Homo sapiens amyloid beta precursor protein binding family B member 2 (APBB2), transcript variant 7, mRNA.

Source:
NCBI, updated 2019-09-24
Taxon:
Homo sapiens (human)
Gene:
APBB2 (323)
Length:
7012
CDS:
286..918

Additional Resources:

NCBI RefSeq record:
NM_001166054.1
NBCI Gene record:
APBB2 (323)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001166054.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000016544 GCCGCCTGCATGTTACGATAT pLKO.1 739 CDS 100% 10.800 15.120 N APBB2 n/a
2 TRCN0000016546 GCTTATGTAGCAAGAGATAAA pLKO.1 175 5UTR 100% 13.200 9.240 N APBB2 n/a
3 TRCN0000442389 GAAACGCCCTGTCACCGAAAT pLKO_005 891 CDS 100% 10.800 7.560 N APBB2 n/a
4 TRCN0000016547 CGATATCAGAAGTGCTTGGTA pLKO.1 754 CDS 100% 3.000 2.100 N APBB2 n/a
5 TRCN0000016545 GAAGAGGAAGTCTTAGTGGAA pLKO.1 574 CDS 100% 2.640 1.848 N APBB2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001166054.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10680 pDONR223 100% 63.3% 63.3% None 0_1ins360;84_86delAGT n/a
2 ccsbBroad304_10680 pLX_304 0% 63.3% 63.3% V5 0_1ins360;84_86delAGT n/a
3 TRCN0000467211 TCGACAATGATCTCATCATGGCAT pLX_317 30.8% 63.3% 63.3% V5 0_1ins360;84_86delAGT n/a
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