Construct: ORF ccsbBroadEn_11018

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001189.1_s300c1, BRDN0000457073
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PCCA (5095)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5095 PCCA propionyl-CoA carboxylase s... XM_017020609.1 98.6% 98.5% (many diffs)
2 human 5095 PCCA propionyl-CoA carboxylase s... NM_000282.4 96.5% 96.5% 1_75del;627A>G
3 human 5095 PCCA propionyl-CoA carboxylase s... NM_001352605.2 94% 94% 1_75del;627A>G;1844_1845ins54
4 human 5095 PCCA propionyl-CoA carboxylase s... NM_001127692.2 92.9% 92.9% 1_75del;105_106ins78;549A>G
5 human 5095 PCCA propionyl-CoA carboxylase s... NM_001178004.1 90% 90.1% 1_75del;627A>G;1898_1899ins141
6 human 5095 PCCA propionyl-CoA carboxylase s... NM_001352606.2 89.9% 89.9% 1_75del;627A>G;1063_1064ins144
7 human 5095 PCCA propionyl-CoA carboxylase s... XM_011521093.1 86.7% 83.2% (many diffs)
8 human 5095 PCCA propionyl-CoA carboxylase s... NM_001352607.2 86.4% 86.5% (many diffs)
9 human 5095 PCCA propionyl-CoA carboxylase s... NM_001352608.2 86.3% 86.4% (many diffs)
10 human 5095 PCCA propionyl-CoA carboxylase s... XM_017020607.1 85.4% 80.6% (many diffs)
11 human 5095 PCCA propionyl-CoA carboxylase s... XR_001749574.1 84.8% (many diffs)
12 human 5095 PCCA propionyl-CoA carboxylase s... NM_001352609.2 84.3% 83.6% (many diffs)
13 human 5095 PCCA propionyl-CoA carboxylase s... XM_017020605.1 83.9% 79.3% (many diffs)
14 human 5095 PCCA propionyl-CoA carboxylase s... XM_017020612.1 83.4% 79.3% (many diffs)
15 human 5095 PCCA propionyl-CoA carboxylase s... NR_148030.2 82% (many diffs)
16 human 5095 PCCA propionyl-CoA carboxylase s... XM_017020611.1 82% 82.3% (many diffs)
17 human 5095 PCCA propionyl-CoA carboxylase s... XM_017020613.1 81.8% 78.5% (many diffs)
18 human 5095 PCCA propionyl-CoA carboxylase s... NR_148031.2 80.7% (many diffs)
19 human 5095 PCCA propionyl-CoA carboxylase s... XM_017020606.1 80.5% 75.9% (many diffs)
20 human 5095 PCCA propionyl-CoA carboxylase s... XR_001749567.1 79.9% (many diffs)
21 human 5095 PCCA propionyl-CoA carboxylase s... XM_017020615.1 78.9% 77.1% (many diffs)
22 human 5095 PCCA propionyl-CoA carboxylase s... NR_148028.2 78% (many diffs)
23 human 5095 PCCA propionyl-CoA carboxylase s... XR_001749568.1 77.4% (many diffs)
24 human 5095 PCCA propionyl-CoA carboxylase s... NR_148027.2 76.5% (many diffs)
25 human 5095 PCCA propionyl-CoA carboxylase s... NR_148029.2 75% (many diffs)
26 human 5095 PCCA propionyl-CoA carboxylase s... XR_931615.1 72.8% (many diffs)
27 human 5095 PCCA propionyl-CoA carboxylase s... XR_001749569.1 72.2% (many diffs)
28 human 5095 PCCA propionyl-CoA carboxylase s... XM_017020616.1 71.8% 71.8% (many diffs)
29 human 5095 PCCA propionyl-CoA carboxylase s... XR_001749576.1 68.2% (many diffs)
30 human 5095 PCCA propionyl-CoA carboxylase s... XR_001749577.1 64.1% (many diffs)
31 human 5095 PCCA propionyl-CoA carboxylase s... NM_001352610.2 58.7% 58.7% 0_1ins870
32 human 5095 PCCA propionyl-CoA carboxylase s... NM_001352611.2 56.1% 56.1% 0_1ins870;899_900ins54
33 human 5095 PCCA propionyl-CoA carboxylase s... NM_001352612.2 51.9% 51.9% 0_1ins870;118_119ins144
34 mouse 110821 Pcca propionyl-Coenzyme A carbox... NM_144844.2 84.7% 87.8% (many diffs)
35 mouse 110821 Pcca propionyl-Coenzyme A carbox... XM_006518430.3 82.4% 85.3% (many diffs)
36 mouse 110821 Pcca propionyl-Coenzyme A carbox... XM_006518432.3 80.3% 84.6% (many diffs)
37 mouse 110821 Pcca propionyl-Coenzyme A carbox... XR_001781088.1 73.3% (many diffs)
38 mouse 110821 Pcca propionyl-Coenzyme A carbox... XM_011244922.2 47.5% 48.5% (many diffs)
39 mouse 110821 Pcca propionyl-Coenzyme A carbox... XR_001781087.1 17.7% (many diffs)
40 mouse 110821 Pcca propionyl-Coenzyme A carbox... XR_383133.3 16.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2175
ORF length:
2109
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGCT GAGCGCGGCG CTGCGGACCC TGAAGCATGT TCTGTACTAT TCAAGACAGT
121GCTTAATGGT GTCCCGTAAT CTTGGTTCAG TGGGATATGA TCCTAATGAA AAAACTTTTG
181ATAAAATTCT TGTTGCTAAT AGAGGAGAAA TTGCATGTCG GGTTATTAGA ACTTGCAAGA
241AGATGGGCAT TAAGACAGTT GCCATCCACA GTGATGTTGA TGCTAGTTCT GTTCATGTGA
301AAATGGCGGA TGAGGCTGTC TGTGTTGGCC CAGCTCCCAC CAGTAAAAGC TACCTCAACA
361TGGATGCCAT CATGGAAGCC ATTAAGAAAA CCAGGGCCCA AGCTGTACAT CCAGGTTATG
421GATTCCTTTC AGAAAACAAA GAATTTGCCA GATGTTTGGC AGCAGAAGAT GTCGTTTTCA
481TTGGACCTGA CACACATGCT ATTCAAGCCA TGGGCGACAA GATTGAAAGC AAATTATTAG
541CTAAGAAAGC AGAGGTTAAT ACAATCCCTG GCTTTGATGG AGTAGTCAAG GATGCAGAAG
601AAGCTGTCAG AATTGCGAGG GAAATTGGCT ACCCTGTCAT GATCAAGGCC TCAGCAGGTG
661GTGGTGGGAA AGGCATGCGC ATTGCTTGGG ATGATGAAGA GACCAGGGAT GGTTTTAGAT
721TGTCATCTCA AGAAGCTGCT TCTAGTTTTG GCGATGATAG ACTACTAATA GAAAAATTTA
781TTGATAATCC TCGTCATATA GAAATCCAGG TTCTAGGTGA TAAACATGGG AATGCTTTAT
841GGCTTAATGA AAGAGAGTGC TCAATTCAGA GAAGAAATCA GAAGGTGGTG GAGGAAGCAC
901CAAGCATTTT TTTGGATGCG GAGACTCGAA GAGCGATGGG AGAACAAGCT GTAGCTCTTG
961CCAGAGCAGT AAAATATTCC TCTGCTGGGA CCGTGGAGTT CCTTGTGGAC TCTAAGAAGA
1021ATTTTTATTT CTTGGAAATG AATACAAGAC TCCAGGTTGA GCATCCTGTC ACAGAATGCA
1081TTACTGGCCT GGACCTAGTC CAGGAAATGA TCCGTGTTGC TAAGGGCTAC CCTCTCAGGC
1141ACAAACAAGC TGATATTCGC ATCAACGGCT GGGCAGTTGA ATGTCGGGTT TATGCTGAGG
1201ACCCCTACAA GTCTTTTGGT TTACCATCTA TTGGGAGATT GTCTCAGTAC CAAGAACCGT
1261TACATCTACC TGGTGTCCGA GTGGACAGTG GCATCCAACC AGGAAGTGAT ATTAGCATTT
1321ATTATGATCC TATGATTTCA AAACTAATCA CATATGGCTC TGATAGAACT GAGGCACTGA
1381AGAGAATGGC AGATGCACTG GATAACTATG TTATTCGAGG TGTTACACAT AATATTGCAT
1441TACTTCGAGA GGTGATAATC AACTCACGCT TTGTAAAAGG AGACATCAGC ACTAAATTTC
1501TCTCCGATGT GTATCCTGAT GGCTTCAAAG GACACATGCT AACCAAGAGT GAGAAGAACC
1561AGTTATTGGC AATAGCATCA TCATTGTTTG TGGCATTCCA GTTAAGAGCA CAACATTTTC
1621AAGAAAATTC AAGAATGCCT GTTATTAAAC CAGACATAGC CAACTGGGAG CTCTCAGTAA
1681AATTGCATGA TAAAGTTCAT ACCGTAGTAG CATCAAACAA TGGGTCAGTG TTCTCGGTGG
1741AAGTTGATGG GTCGAAACTA AATGTGACCA GCACGTGGAA CCTGGCTTCG CCCTTATTGT
1801CTGTCAGCGT TGATGGCACT CAGAGGACTG TCCAGTGTCT TTCTCGAGAA GCAGGTGGAA
1861ACATGAGCAT TCAGTTTCTT GGTACAGTGT ACAAGGTGAA TATCTTAACC AGACTTGCCG
1921CAGAATTGAA CAAATTTATG CTGGAAAAAG TGACTGAGGA CACAAGCAGT GTTCTGCGTT
1981CCCCGATGCC CGGAGTGGTG GTGGCCGTCT CTGTCAAGCC TGGAGACGCG GTAGCAGAAG
2041GTCAAGAAAT TTGTGTGATT GAAGCCATGA AAATGCAGAA TAGTATGACA GCTGGGAAAA
2101CTGGCACGGT GAAATCTGTG CACTGTCAAG CTGGAGACAC AGTTGGAGAA GGGGATCTGC
2161TCGTGGAGCT GGAATGCCCA ACTTTCTTGT ACAAAGTTGG cattataaga aagcattgct
2221tatcaatttg ttgcaacgaa c

Download FASTA (ORF) (Full)