Construct: ORF ccsbBroadEn_11046
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012718.1_s300c1, BRDN0000386587
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- POU2F1 (5451)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5451 | POU2F1 | POU class 2 homeobox 1 | XM_011509654.3 | 100% | 100% | |
| 2 | human | 5451 | POU2F1 | POU class 2 homeobox 1 | XM_024447727.1 | 100% | 100% | |
| 3 | human | 5451 | POU2F1 | POU class 2 homeobox 1 | NM_001198783.2 | 98.4% | 98.4% | 1_36del |
| 4 | human | 5451 | POU2F1 | POU class 2 homeobox 1 | NM_001365848.1 | 98% | 95.6% | (many diffs) |
| 5 | human | 5451 | POU2F1 | POU class 2 homeobox 1 | NM_001365849.1 | 98% | 95.6% | (many diffs) |
| 6 | human | 5451 | POU2F1 | POU class 2 homeobox 1 | NM_002697.4 | 96.9% | 96.9% | 1_69del |
| 7 | human | 5451 | POU2F1 | POU class 2 homeobox 1 | XM_011509653.2 | 96.6% | 96.6% | 1_78del |
| 8 | human | 5451 | POU2F1 | POU class 2 homeobox 1 | NM_001198786.2 | 88.7% | 88.7% | 1_69del;401_402ins189 |
| 9 | human | 5451 | POU2F1 | POU class 2 homeobox 1 | XM_011509655.1 | 83.7% | 83.4% | (many diffs) |
| 10 | human | 5451 | POU2F1 | POU class 2 homeobox 1 | XM_017001508.2 | 46.1% | 46% | 1_78del;1136_1137insAG;1143_1144ins1162 |
| 11 | human | 5451 | POU2F1 | POU class 2 homeobox 1 | NR_037163.2 | 15.9% | 1_182del;2412_13944del | |
| 12 | mouse | 18986 | Pou2f1 | POU domain, class 2, transc... | XM_017319360.1 | 90.1% | 93.1% | (many diffs) |
| 13 | mouse | 18986 | Pou2f1 | POU domain, class 2, transc... | NM_198933.3 | 88.7% | 91.6% | (many diffs) |
| 14 | mouse | 18986 | Pou2f1 | POU domain, class 2, transc... | NM_198932.3 | 87.5% | 90.3% | (many diffs) |
| 15 | mouse | 18986 | Pou2f1 | POU domain, class 2, transc... | XM_006496704.2 | 87.3% | 90.2% | (many diffs) |
| 16 | mouse | 18986 | Pou2f1 | POU domain, class 2, transc... | XM_011238767.2 | 87.3% | 90.2% | (many diffs) |
| 17 | mouse | 18986 | Pou2f1 | POU domain, class 2, transc... | XM_011238769.2 | 87.3% | 90.2% | (many diffs) |
| 18 | mouse | 18986 | Pou2f1 | POU domain, class 2, transc... | NM_198934.3 | 86% | 88.8% | (many diffs) |
| 19 | mouse | 18986 | Pou2f1 | POU domain, class 2, transc... | NM_011137.3 | 84.8% | 87.6% | (many diffs) |
| 20 | mouse | 18986 | Pou2f1 | POU domain, class 2, transc... | XR_373598.1 | 16.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2295
- ORF length:
- 2229
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGAA CAATCCGTCA GAAACCAGTA AACCATCTAT GGAGAGTGGA GATGGCAACA 121 CAGGCACACA AACCAATGGT CTGGACTTTC AGAAGCAGCC TGTGCCTGTA GGAGGAGCAA 181 TCTCAACAGC CCAGGCGCAG GCTTTCCTTG GACATCTCCA TCAGGTCCAA CTCGCTGGAA 241 CAAGTTTACA GGCTGCTGCT CAGTCTTTAA ATGTACAGTC TAAATCTAAT GAAGAATCGG 301 GGGATTCGCA GCAGCCAAGC CAGCCTTCCC AGCAGCCTTC AGTGCAGGCA GCCATTCCCC 361 AGACCCAGCT TATGCTAGCT GGAGGACAGA TAACTGGGCT TACTTTGACG CCTGCCCAGC 421 AACAGTTACT ACTCCAGCAG GCACAGGCAC AGGCACAGCT GCTGGCTGCT GCAGTGCAGC 481 AGCACTCCGC CAGCCAGCAG CACAGTGCTG CTGGAGCCAC CATCTCCGCC TCTGCTGCCA 541 CGCCCATGAC GCAGATCCCC CTGTCTCAGC CCATACAGAT CGCACAGGAT CTTCAACAAC 601 TGCAACAGCT TCAACAGCAG AATCTCAACC TGCAACAGTT TGTGTTGGTG CATCCAACCA 661 CCAATTTGCA GCCAGCGCAG TTTATCATCT CACAGACGCC CCAGGGCCAG CAGGGTCTCC 721 TGCAAGCGCA AAATCTTCTA ACGCAACTAC CTCAGCAAAG CCAAGCCAAC CTCCTACAGT 781 CGCAGCCAAG CATCACCCTC ACCTCCCAGC CAGCAACCCC AACACGCACA ATAGCAGCAA 841 CCCCAATTCA GACACTTCCA CAGAGCCAGT CAACACCAAA GCGAATTGAT ACTCCCAGCT 901 TGGAGGAGCC CAGTGACCTT GAGGAGCTTG AGCAGTTTGC CAAGACCTTC AAACAAAGAC 961 GAATCAAACT TGGATTCACT CAGGGTGATG TTGGGCTCGC TATGGGGAAA CTATATGGAA 1021 ATGACTTCAG CCAAACTACC ATCTCTCGAT TTGAAGCCTT GAACCTCAGC TTTAAGAACA 1081 TGTGCAAGTT GAAGCCACTT TTAGAGAAGT GGCTAAATGA TGCAGAGAAC CTCTCATCTG 1141 ATTCGTCCCT CTCCAGCCCA AGTGCCCTGA ATTCTCCAGG AATTGAGGGC TTGAGCCGTA 1201 GGAGGAAGAA ACGCACCAGC ATAGAGACCA ACATCCGTGT GGCCTTAGAG AAGAGTTTCT 1261 TGGAGAATCA AAAGCCTACC TCGGAAGAGA TCACTATGAT TGCTGATCAG CTCAATATGG 1321 AAAAAGAGGT GATTCGTGTT TGGTTCTGTA ACCGCCGCCA GAAAGAAAAA AGAATCAACC 1381 CACCAAGCAG TGGTGGGACC AGCAGCTCAC CTATTAAAGC AATTTTCCCC AGCCCAACTT 1441 CACTGGTGGC GACCACACCA AGCCTTGTGA CTAGCAGTGC AGCAACTACC CTCACAGTCA 1501 GCCCTGTCCT CCCTCTGACC AGTGCTGCTG TGACGAATCT TTCAGTTACA GGCACTTCAG 1561 ACACCACCTC CAACAACACA GCAACCGTGA TTTCCACAGC GCCTCCAGCT TCCTCAGCAG 1621 TCACGTCCCC CTCTCTGAGT CCCTCCCCTT CTGCCTCAGC CTCCACCTCC GAGGCATCCA 1681 GTGCCAGTGA GACCAGCACA ACACAGACCA CCTCCACTCC TTTGTCCTCC CCTCTTGGGA 1741 CCAGCCAGGT GATGGTGACA GCATCAGGTT TGCAAACAGC AGCAGCTGCT GCCCTTCAAG 1801 GAGCTGCACA GTTGCCAGCA AATGCCAGTC TTGCTGCCAT GGCAGCTGCT GCAGGACTAA 1861 ACCCAAGCCT GATGGCACCC TCACAGTTTG CGGCTGGAGG TGCCTTACTC AGTCTGAATC 1921 CAGGGACCCT GAGCGGTGCT CTCAGCCCAG CTCTAATGAG CAACAGTACA CTGGCAACTA 1981 TTCAAGCTCT TGCTTCTGGT GGCTCTCTTC CAATAACATC ACTTGATGCA ACTGGGAACC 2041 TGGTATTTGC CAATGCGGGA GGAGCCCCCA ACATCGTGAC TGCCCCTCTG TTCCTGAACC 2101 CTCAGAACCT CTCTCTGCTC ACCAGCAACC CTGTTAGCTT GGTCTCTGCC GCCGCAGCAT 2161 CTGCAGGGAA CTCTGCACCT GTAGCCAGCC TTCACGCCAC CTCCACCTCT GCTGAGTCCA 2221 TCCAGAACTC TCTCTTCACA GTGGCCTCTG CCAGCGGGGC TGCGTCCACC ACCACCACCG 2281 CCTCCAAGGC ACAGTGCCCA ACTTTCTTGT ACAAAGTtgg cattataaga aagcattgct 2341 tatcaatttg ttgcaacgaa c