Transcript: Mouse XM_011238769.2

PREDICTED: Mus musculus POU domain, class 2, transcription factor 1 (Pou2f1), transcript variant X4, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Pou2f1 (18986)
Length:
13200
CDS:
300..2612

Additional Resources:

NCBI RefSeq record:
XM_011238769.2
NBCI Gene record:
Pou2f1 (18986)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_011238769.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000240640 ACAACACGGCCACGGTGATTT pLKO_005 1891 CDS 100% 13.200 18.480 N Pou2f1 n/a
2 TRCN0000020768 CCAGTCAACACCAAAGCGAAT pLKO.1 1103 CDS 100% 4.050 5.670 N POU2F1 n/a
3 TRCN0000240636 TAAGAACATGTGCAAGTTAAA pLKO_005 1310 CDS 100% 13.200 10.560 N Pou2f1 n/a
4 TRCN0000240638 CAGTGAAGAGTCGGGAGATTC pLKO_005 521 CDS 100% 10.800 8.640 N Pou2f1 n/a
5 TRCN0000175063 GTACAGTCTAAATCCAGTGAA pLKO.1 507 CDS 100% 4.950 3.960 N Pou2f1 n/a
6 TRCN0000176291 CAACGATTCAAGCTCTTGCTT pLKO.1 2290 CDS 100% 3.000 2.400 N Pou2f1 n/a
7 TRCN0000232120 GCAACTGGGAACCTGGTATTT pLKO_005 2343 CDS 100% 13.200 9.240 N POU2F1 n/a
8 TRCN0000240639 GCAACTGGGAACCTGGTATTT pLKO_005 2343 CDS 100% 13.200 9.240 N Pou2f1 n/a
9 TRCN0000240637 TTCCTCACTACAGGGTGATAG pLKO_005 2643 3UTR 100% 10.800 7.560 N Pou2f1 n/a
10 TRCN0000175090 GCTGCTCAGTCTTTAAATGTA pLKO.1 489 CDS 100% 5.625 3.938 N Pou2f1 n/a
11 TRCN0000173948 GCCCTAATGAGCAACAGTACA pLKO.1 2265 CDS 100% 4.950 3.465 N Pou2f1 n/a
12 TRCN0000176221 GCTGAACAGCTCAATATGGAA pLKO.1 1545 CDS 100% 3.000 2.100 N Pou2f1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011238769.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11046 pDONR223 100% 87.3% 90.2% None (many diffs) n/a
2 ccsbBroad304_11046 pLX_304 0% 87.3% 90.2% V5 (many diffs) n/a
3 TRCN0000471094 GGGAGACTGCATGAGGCTTGATTA pLX_317 19.3% 87.3% 90.2% V5 (many diffs) n/a
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