Construct: ORF ccsbBroadEn_13072

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008998.1_s300c1, BRDN0000385914
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ZFYVE27 (118813)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 118813 ZFYVE27 zinc finger FYVE-type conta... XM_017015650.1 99.8% 99.7% 209G>T;951C>A
2 human 118813 ZFYVE27 zinc finger FYVE-type conta... XM_011539254.2 98.3% 98.2% 209G>T;599_613del;966C>A
3 human 118813 ZFYVE27 zinc finger FYVE-type conta... XM_011539255.2 98.3% 98.2% 209G>T;599_613del;966C>A
4 human 118813 ZFYVE27 zinc finger FYVE-type conta... XM_017015651.2 94% 93.8% (many diffs)
5 human 118813 ZFYVE27 zinc finger FYVE-type conta... XM_017015652.1 94% 93.8% (many diffs)
6 human 118813 ZFYVE27 zinc finger FYVE-type conta... XM_005269508.4 92.7% 92.5% (many diffs)
7 human 118813 ZFYVE27 zinc finger FYVE-type conta... XM_005269509.3 92.7% 92.5% (many diffs)
8 human 118813 ZFYVE27 zinc finger FYVE-type conta... XM_017015653.2 92% 91.8% (many diffs)
9 human 118813 ZFYVE27 zinc finger FYVE-type conta... XM_017015654.1 92% 91.8% (many diffs)
10 human 118813 ZFYVE27 zinc finger FYVE-type conta... NM_001174121.1 87.7% 87.6% (many diffs)
11 human 118813 ZFYVE27 zinc finger FYVE-type conta... NM_001174119.1 84.6% 84.1% (many diffs)
12 human 118813 ZFYVE27 zinc finger FYVE-type conta... NM_144588.7 83.2% 83.2% 1_204del;413G>T;1155C>A
13 human 118813 ZFYVE27 zinc finger FYVE-type conta... XM_005269504.3 83.2% 83.2% 1_204del;413G>T;1155C>A
14 human 118813 ZFYVE27 zinc finger FYVE-type conta... XM_017015648.1 82.8% 76.1% (many diffs)
15 human 118813 ZFYVE27 zinc finger FYVE-type conta... XM_017015655.1 82.8% 76.1% (many diffs)
16 human 118813 ZFYVE27 zinc finger FYVE-type conta... NM_001002261.3 82.2% 82.2% (many diffs)
17 human 118813 ZFYVE27 zinc finger FYVE-type conta... XM_005269502.3 82.2% 82.2% (many diffs)
18 human 118813 ZFYVE27 zinc finger FYVE-type conta... XM_005269503.3 82.2% 82.2% (many diffs)
19 human 118813 ZFYVE27 zinc finger FYVE-type conta... XM_005269510.3 81.7% 75% (many diffs)
20 human 118813 ZFYVE27 zinc finger FYVE-type conta... XM_005269511.3 81.7% 75% (many diffs)
21 human 118813 ZFYVE27 zinc finger FYVE-type conta... NM_001002262.4 81.5% 81.5% (many diffs)
22 human 118813 ZFYVE27 zinc finger FYVE-type conta... XM_005269506.3 81.5% 81.5% (many diffs)
23 human 118813 ZFYVE27 zinc finger FYVE-type conta... NM_001174120.1 80.9% 74.1% (many diffs)
24 human 118813 ZFYVE27 zinc finger FYVE-type conta... XM_005269505.4 80.6% 80.5% (many diffs)
25 human 118813 ZFYVE27 zinc finger FYVE-type conta... XM_017015647.1 80.6% 80.5% (many diffs)
26 human 118813 ZFYVE27 zinc finger FYVE-type conta... XM_017015649.1 79.7% 73.1% (many diffs)
27 human 118813 ZFYVE27 zinc finger FYVE-type conta... XM_011539252.2 77.2% 77.4% (many diffs)
28 human 118813 ZFYVE27 zinc finger FYVE-type conta... XM_011539253.2 75.5% 75.7% (many diffs)
29 human 118813 ZFYVE27 zinc finger FYVE-type conta... NM_001174122.1 72.7% 65.7% (many diffs)
30 human 118813 ZFYVE27 zinc finger FYVE-type conta... XM_017015644.1 67.7% 67.5% (many diffs)
31 human 118813 ZFYVE27 zinc finger FYVE-type conta... XM_017015646.1 66.8% 66.6% (many diffs)
32 human 118813 ZFYVE27 zinc finger FYVE-type conta... XM_017015645.1 66% 65.8% (many diffs)
33 human 118813 ZFYVE27 zinc finger FYVE-type conta... XR_945594.2 57.2% (many diffs)
34 human 118813 ZFYVE27 zinc finger FYVE-type conta... XR_945597.2 56.4% (many diffs)
35 human 118813 ZFYVE27 zinc finger FYVE-type conta... XR_002956957.1 23.3% (many diffs)
36 human 118813 ZFYVE27 zinc finger FYVE-type conta... XR_002956956.1 22.5% (many diffs)
37 mouse 319740 Zfyve27 zinc finger, FYVE domain co... NM_177319.3 71% 73.5% (many diffs)
38 mouse 319740 Zfyve27 zinc finger, FYVE domain co... NM_001164531.1 69.4% 71.8% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1095
ORF length:
1029
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGCC TTTGTGTTCC TTGCTGACCT GCCTGGGCCT CAACGTCTTG TTCCTCACTT
121TGAATGAGGG TGCATGGTAC TCAGTAGGTG CCCTGATGAT TTCAGTGCCC GCCCTGCTGG
181GCTACCTTCA GGAGGTTTGC CGGGCACGGC TGCCTGATTC CGAGCTGATG CGGAGGAAGT
241ATCATAGCGT GAGGCAGGAG GACCTGCAGA GAGTTCGCCT GTCTCGTCCC GAGGCCGTGG
301CTGAGGTGAA GAGCTTCTTG ATCCAGCTGG AGGCCTTCCT GAGCCGCCTG TGCTGCACAT
361GTGAAGCCGC CTACCGCGTG CTGCACTGGG AGAACCCCGT CGTGTCCTCA CAGTTCTATG
421GGGCTCTTCT GGGCACAGTC TGCATGCTGT ATTTGCTGCC ACTCTGCTGG GTTCTCACCC
481TTTTAAACAG CACGCTCTTT CTGGGGAATG TGGAGTTCTT CCGAGTTGTG TCTGAGTACA
541GGGCATCTCT GCAGCAGAGG ATGAACCCAA AGCAGGAAGA GCATGCCTTT GAGAGTCCTC
601CACCACCAGA TGTTGGGGGG AAGGATGGTC TGATGGACAG CACGCCTGCC CTCACACCCA
661CGGAGGACCT CACACCGGGC AGCGTGGAGG AGGCTGAGGA GGCTGAGCCA GATGAAGAGT
721TTAAAGATGC GATTGAGGAG ACCCACTTGG TGGTGCTGGA GGATGATGAG GGCGCCCCGT
781GCCCAGCAGA GGATGAGCTG GCCCTGCAGG ACAACGGGTT CCTGAGCAAG AATGAGGTGC
841TGCGCAGCAA GGTGTCTCGG CTCACGGAGC GGCTCCGCAA GCGCTACCCC ACCAACAACT
901TCGGGAACTG CACGGGCTGC TCGGCCACCT TCTCAGTGCT GAAGAAGAGG CGGAGCTGCA
961GTAATTGTGG AAACAGCTTC TGCTCTCGAT GCTGCTCCTT CAAGGTGCCC AAGTCATCCA
1021TGGGGGCCAC AGCCCCTGAA GCCCAGAGGG AGACTGTGTT TGTGTGTGCC TCGTGTAACC
1081AGACCTTGAG CAAGTGCCCA ACTTTCTTGT ACAAAGTtgg cattataaga aagcattgct
1141tatcaatttg ttgcaacgaa c

Download FASTA (ORF) (Full)