Transcript: Human XR_945594.2

PREDICTED: Homo sapiens zinc finger FYVE-type containing 27 (ZFYVE27), transcript variant X8, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ZFYVE27 (118813)
Length:
1449
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_945594.2
NBCI Gene record:
ZFYVE27 (118813)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_945594.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000137025 CAACGTCTTGTTCCTCACTTT pLKO.1 435 3UTR 100% 4.950 3.465 N ZFYVE27 n/a
2 TRCN0000136788 CAACTTGGTTCTCTCCTACAA pLKO.1 309 3UTR 100% 4.950 3.465 N ZFYVE27 n/a
3 TRCN0000137843 GCAGATGCCTTTGTGTTCCTT pLKO.1 396 3UTR 100% 3.000 2.100 N ZFYVE27 n/a
4 TRCN0000138786 GAAGAGCTTCTTGATCCAGCT pLKO.1 642 3UTR 100% 2.160 1.512 N ZFYVE27 n/a
5 TRCN0000138680 GAAGTATCATAGCGTGAGGCA pLKO.1 570 3UTR 100% 0.660 0.462 N ZFYVE27 n/a
6 TRCN0000138586 CTTCCGAGTTGTGTCTGAGTA pLKO.1 852 3UTR 100% 4.950 2.970 N ZFYVE27 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_945594.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13072 pDONR223 100% 57.2% None (many diffs) n/a
2 ccsbBroad304_13072 pLX_304 0% 57.2% V5 (many diffs) n/a
3 TRCN0000473164 ACTATGTATCCCCCATGCCTCGCG pLX_317 41.5% 57.2% V5 (many diffs) n/a
Download CSV