Construct: ORF ccsbBroadEn_13107

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012536.1_s300c1, BRDN0000391014
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MSI2 (124540)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 124540 MSI2 musashi RNA binding protein 2 XM_011524286.2 60.7% 58.2% (many diffs)
2 human 124540 MSI2 musashi RNA binding protein 2 NM_170721.2 60.1% 60.1% 1_300del
3 human 124540 MSI2 musashi RNA binding protein 2 NM_001322251.2 59.2% 59.2% 1_312del
4 human 124540 MSI2 musashi RNA binding protein 2 XM_017024149.2 56.7% 54.4% (many diffs)
5 human 124540 MSI2 musashi RNA binding protein 2 XM_011524287.3 51.9% 43.1% (many diffs)
6 human 124540 MSI2 musashi RNA binding protein 2 XM_017024148.1 49.6% 47.7% (many diffs)
7 human 124540 MSI2 musashi RNA binding protein 2 NM_001322250.1 45.3% 43.5% (many diffs)
8 human 124540 MSI2 musashi RNA binding protein 2 XM_017024147.2 43.1% 38.7% (many diffs)
9 human 124540 MSI2 musashi RNA binding protein 2 NM_138962.4 42.8% 42.9% (many diffs)
10 human 124540 MSI2 musashi RNA binding protein 2 XM_005257014.3 42.4% 40.7% (many diffs)
11 human 124540 MSI2 musashi RNA binding protein 2 XM_005257015.3 42.4% 40.7% (many diffs)
12 mouse 76626 Msi2 musashi RNA-binding protein 2 XM_011249305.2 49.6% 51.2% (many diffs)
13 mouse 76626 Msi2 musashi RNA-binding protein 2 XM_011249304.2 46.8% 48.9% (many diffs)
14 mouse 76626 Msi2 musashi RNA-binding protein 2 XM_006534442.3 40.5% 42.9% (many diffs)
15 mouse 76626 Msi2 musashi RNA-binding protein 2 XM_017314830.1 39.8% 41.2% (many diffs)
16 mouse 76626 Msi2 musashi RNA-binding protein 2 XM_006534441.3 38.6% 39.9% (many diffs)
17 mouse 76626 Msi2 musashi RNA-binding protein 2 NM_054043.3 38.4% 40.7% (many diffs)
18 mouse 76626 Msi2 musashi RNA-binding protein 2 XM_011249302.1 37.8% 39.1% (many diffs)
19 mouse 76626 Msi2 musashi RNA-binding protein 2 XM_006534440.2 37.8% 40% (many diffs)
20 mouse 76626 Msi2 musashi RNA-binding protein 2 XM_006534438.2 37.2% 38.5% (many diffs)
21 mouse 76626 Msi2 musashi RNA-binding protein 2 XM_006534439.2 37.2% 38.5% (many diffs)
22 mouse 76626 Msi2 musashi RNA-binding protein 2 XM_006534434.2 36% 38.1% (many diffs)
23 mouse 76626 Msi2 musashi RNA-binding protein 2 XM_006534435.2 36% 38.1% (many diffs)
24 mouse 76626 Msi2 musashi RNA-binding protein 2 XM_006534431.2 35.5% 36.7% (many diffs)
25 mouse 76626 Msi2 musashi RNA-binding protein 2 XM_006534432.2 35.5% 36.7% (many diffs)
26 mouse 76626 Msi2 musashi RNA-binding protein 2 XR_388550.2 6.1% (many diffs)
27 mouse 76626 Msi2 musashi RNA-binding protein 2 XR_879764.1 6.1% (many diffs)
28 mouse 76626 Msi2 musashi RNA-binding protein 2 XR_001780073.1 6.1% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
519
ORF length:
453
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGT CACAAGAACA AAGAAAATAT TTGTAGGCGG GTTATCTGCG AACACAGTAG
121TGGAAGATGT AAAGCAATAT TTCGAGCAGT TTGGCAAGGT GGAAGATGCA ATGCTGATGT
181TTGATAAAAC TACCAACAGG CACAGAGGGT TTGGCTTTGT CACTTTTGAG AATGAAGATG
241TTGTGGAGAA AGTCTGTGAG ATTCATTTCC ATGAAATCAA TAATAAAATG GTAGAATGTA
301AGAAAGCTCA GCCGAAAGAA GTCATGTTCC CACCTGGGAC AAGAGGCCGG GCCCGGGGAC
361TGCCTTACAC CATGGACGCG TTCATGCTTG GCATGGGGAT GCTGGGATAT CCCAACTTCG
421TGGCGACCTA TGGCCGTGGC TACCCCGGAT TTGCTCCAAG CTATGGCTAT CAGTTCCCAG
481ACTATTTGCC GGTTTCACAA GACATAATTT TTATCAACTA CCCAACTTTC TTGTACAAAG
541Ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaac

Download FASTA (ORF) (Full)