Construct: ORF ccsbBroadEn_13444

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004245.1_s300c1, BRDN0000455359
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
AKR7L (246181)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 246181 AKR7L aldo-keto reductase family ... NM_001348421.1 46.2% 32.3% 1_337del;508_704del
2 human 22977 AKR7A3 aldo-keto reductase family ... NM_012067.3 44.9% 30.8% (many diffs)
3 human 22977 AKR7A3 aldo-keto reductase family ... XM_017000714.2 42.6% 18.9% (many diffs)
4 human 8574 AKR7A2 aldo-keto reductase family ... NM_001320979.1 40.8% 30.5% (many diffs)
5 human 8574 AKR7A2 aldo-keto reductase family ... NM_003689.3 40.2% 28.4% (many diffs)
6 human 22977 AKR7A3 aldo-keto reductase family ... XM_011541046.3 34.7% 15.4% (many diffs)
7 human 8574 AKR7A2 aldo-keto reductase family ... XR_946785.2 34.4% (many diffs)
8 human 246182 AKR7A2P1 aldo-keto reductase family ... NR_002796.2 32.6% (many diffs)
9 human 22977 AKR7A3 aldo-keto reductase family ... XR_001737055.1 26% (many diffs)
10 human 246181 AKR7L aldo-keto reductase family ... NR_040289.2 21% 1_385del;845_2183del
11 human 246181 AKR7L aldo-keto reductase family ... NR_040288.2 19.2% 1_385del;556_752del;1042_2380del
12 mouse 110198 Akr7a5 aldo-keto reductase family ... NM_025337.3 36.6% 25.4% (many diffs)
13 mouse 110198 Akr7a5 aldo-keto reductase family ... XR_390721.3 35.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
528
ORF length:
459
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG
61TTGGCACCAT GCACCTGACC ACAGCGCCCC GGTGGAAGAG ACACTGCGTG CCTGCCACCA
121GCTGCACCAG GAGGGCAAGT TCGTGGAGCT TGGCCTCTCC AACTATGCCG CCTGGGAAGT
181GGCCGAGATC TGTACCCTCT GCAAGAGCAA CGGCTGGATC CTGCCCACTG TGTACCAGCT
241TCTGGAAGGA GCACCACTTC GAGGGCATTG CCCTGGTGGA GAAGGCCCTG CAGGCCGCGT
301ATGGCGCCAG CGCTCCCAGC ATGACCTCGG CCGCCCTCCG GTGGATGTAC CACCACTCAC
361AGCTGCAGGG TGCCCACGGG GACGCGGTCA TCCTGGGCAT GTCCAGCCTG GAGCAGCTGG
421AGCAGAACTT GGCAGCGGCA GAGGAAGGGC CCCTGGAGCC GGCTGTCGTG GACGCCTTTA
481ATCAAGCCTG GCATTTGTTT GCCCACGAAT GTCCCAACTA CTTCATCTTG CCAACTTTCT
541TGTACAAAGT TGGcattata agaaagcatt gcttatcaat ttgttgcaac gaac

Download FASTA (ORF) (Full)