Construct: ORF ccsbBroadEn_13922
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010879.1_s300c1, BRDN0000384917
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- POLR2K (5440)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5440 | POLR2K | RNA polymerase II subunit K | NM_005034.4 | 99.4% | 98.2% | 173G>C |
| 2 | mouse | 17749 | Polr2k | polymerase (RNA) II (DNA di... | NM_023127.3 | 91.9% | 96.5% | (many diffs) |
| 3 | mouse | 102641086 | LOC102641086 | DNA-directed RNA polymerase... | XM_006536721.2 | 91.3% | 94.8% | (many diffs) |
| 4 | mouse | 100862456 | LOC100862456 | DNA-directed RNA polymerase... | XM_006537451.2 | 91.3% | 94.8% | (many diffs) |
| 5 | mouse | 17749 | Polr2k | polymerase (RNA) II (DNA di... | NM_001039368.2 | 53.8% | 56.5% | (many diffs) |
| 6 | mouse | 108167692 | Gm46136 | predicted gene, 46136 | XR_001779328.1 | 1.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 240
- ORF length:
- 174
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGA CACCCAGAAG GACGTTCAAC CTCCAAAGCA GCAACCAATG ATATATATCT 121 GTGGAGAGTG TCACACAGAA AATGAAATAA AATCTAGGGA TCCAATCAGA TGCAGAGAAT 181 GTGGATACAG AATAATGTAC AAGAAAAGGA CTAAAAGATT GGTCGTTTTT GATGCTCCAT 241 GCCCAACTTT CTTGTACAAA GTtggcatta taagaaagca ttgcttatca atttgttgca 301 acgaac