Construct: ORF ccsbBroadEn_14622

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF019049.1_s300c1, BRDN0000458667
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
EGFR (1956)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1956 EGFR epidermal growth factor rec... NM_005228.5 99.9% 100% 2709T>C
2 human 1956 EGFR epidermal growth factor rec... NM_005228.3:c.2239_2247del 99.7% 99.7% 2235_2236insGAATTAAGA
3 human 1956 EGFR epidermal growth factor rec... NM_005228.3:c.2240_2254del 99.5% 99.5% 2237_2238insATTAAGAGAAGCAAC
4 human 1956 EGFR epidermal growth factor rec... NM_001346899.1 96.2% 96.1% 424_425ins135;2574T>C
5 human 1956 EGFR epidermal growth factor rec... NM_001346900.2 95.5% 95.6% 0_1ins159;2550T>C
6 human 1956 EGFR epidermal growth factor rec... NM_001346898.2 92.5% 90.3% (many diffs)
7 human 1956 EGFR epidermal growth factor rec... NM_001346897.2 88.8% 86.6% (many diffs)
8 human 1956 EGFR epidermal growth factor rec... NM_001346941.2 77.4% 75.1% (many diffs)
9 human 1956 EGFR epidermal growth factor rec... NM_201284.2 56.5% 50.7% (many diffs)
10 human 1956 EGFR epidermal growth factor rec... NM_201282.2 51.8% 51.8% 1881G>A;1883C>G;1884_1885ins1746
11 human 1956 EGFR epidermal growth factor rec... NM_201283.1 33.4% 33.3% 1206_1207insG;1212_1213delGAinsTT;1215_1216ins2414
12 mouse 13649 Egfr epidermal growth factor rec... NM_207655.2 84.7% 90.3% (many diffs)
13 mouse 13649 Egfr epidermal growth factor rec... NM_007912.4 44.9% 46.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
3699
ORF length:
3630
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag
61ttggcaccat gcgaccctcc gggacggccg gggcagcgct cctggcgctg ctggctgcgc
121tctgcccggc gagtcgggct ctggaggaaa agaaagtttg ccaaggcacg agtaacaagc
181tcacgcagtt gggcactttt gaagatcatt ttctcagcct ccagaggatg ttcaataact
241gtgaggtggt ccttgggaat ttggaaatta cctatgtgca gaggaattat gatctttcct
301tcttaaagac catccaggag gtggctggtt atgtcctcat tgccctcaac acagtggagc
361gaattccttt ggaaaacctg cagatcatca gaggaaatat gtactacgaa aattcctatg
421ccttagcagt cttatctaac tatgatgcaa ataaaaccgg actgaaggag ctgcccatga
481gaaatttaca ggaaatcctg catggcgccg tgcggttcag caacaaccct gccctgtgca
541acgtggagag catccagtgg cgggacatag tcagcagtga ctttctcagc aacatgtcga
601tggacttcca gaaccacctg ggcagctgcc aaaagtgtga tccaagctgt cccaatggga
661gctgctgggg tgcaggagag gagaactgcc agaaactgac caaaatcatc tgtgcccagc
721agtgctccgg gcgctgccgt ggcaagtccc ccagtgactg ctgccacaac cagtgtgctg
781caggctgcac aggcccccgg gagagcgact gcctggtctg ccgcaaattc cgagacgaag
841ccacgtgcaa ggacacctgc cccccactca tgctctacaa ccccaccacg taccagatgg
901atgtgaaccc cgagggcaaa tacagctttg gtgccacctg cgtgaagaag tgtccccgta
961attatgtggt gacagatcac ggctcgtgcg tccgagcctg tggggccgac agctatgaga
1021tggaggaaga cggcgtccgc aagtgtaaga agtgcgaagg gccttgccgc aaagtgtgta
1081acggaatagg tattggtgaa tttaaagact cactctccat aaatgctacg aatattaaac
1141acttcaaaaa ctgcacctcc atcagtggcg atctccacat cctgccggtg gcatttaggg
1201gtgactcctt cacacatact cctcctctgg atccacagga actggatatt ctgaaaaccg
1261taaaggaaat cacagggttt ttgctgattc aggcttggcc tgaaaacagg acggacctcc
1321atgcctttga gaacctagaa atcatacgcg gcaggaccaa gcaacatggt cagttttctc
1381ttgcagtcgt cagcctgaac ataacatcct tgggattacg ctccctcaag gagataagtg
1441atggagatgt gataatttca ggaaacaaaa atttgtgcta tgcaaataca ataaactgga
1501aaaaactgtt tgggacctcc ggtcagaaaa ccaaaattat aagcaacaga ggtgaaaaca
1561gctgcaaggc cacaggccag gtctgccatg ccttgtgctc ccccgagggc tgctggggcc
1621cggagcccag ggactgcgtc tcttgccgga atgtcagccg aggcagggaa tgcgtggaca
1681agtgcaacct tctggagggt gagccaaggg agtttgtgga gaactctgag tgcatacagt
1741gccacccaga gtgcctgcct caggccatga acatcacctg cacaggacgg ggaccagaca
1801actgtatcca gtgtgcccac tacattgacg gcccccactg cgtcaagacc tgcccggcag
1861gagtcatggg agaaaacaac accctggtct ggaagtacgc agacgccggc catgtgtgcc
1921acctgtgcca tccaaactgc acctacggat gcactgggcc aggtcttgaa ggctgtccaa
1981cgaatgggcc taagatcccg tccatcgcca ctgggatggt gggggccctc ctcttgctgc
2041tggtggtggc cctggggatc ggcctcttca tgcgaaggcg ccacatcgtt cggaagcgca
2101cgctgcggag gctgctgcag gagagggagc ttgtggagcc tcttacaccc agtggagaag
2161ctcccaacca agctctcttg aggatcttga aggaaactga attcaaaaag atcaaagtgc
2221tgggctccgg tgcgttcggc acggtgtata agggactctg gatcccagaa ggtgagaaag
2281ttaaaattcc cgtcgctatc aaggaattaa gagaagcaac atctccgaaa gccaacaagg
2341aaatcctcga tgaagcctac gtgatggcca gcgtggacaa cccccacgtg tgccgcctgc
2401tgggcatctg cctcacctcc accgtgcagc tcatcacgca gctcatgccc ttcggctgcc
2461tcctggacta tgtccgggaa cacaaagaca atattggctc ccagtacctg ctcaactggt
2521gtgtgcagat cgcaaagggc atgaactact tggaggaccg tcgcttggtg caccgcgacc
2581tggcagccag gaacgtactg gtgaaaacac cgcagcatgt caagatcaca gattttgggc
2641tggccaaact gctgggtgcg gaagagaaag aataccatgc agaaggaggc aaagtgccta
2701tcaagtggat ggcattggaa tcaattttac acagaatcta tacccaccag agtgatgtct
2761ggagctacgg ggtgaccgtt tgggagttga tgacctttgg atccaagcca tatgacggaa
2821tccctgccag cgagatctcc tccatcctgg agaaaggaga acgcctccct cagccaccca
2881tatgtaccat cgatgtctac atgatcatgg tcaagtgctg gatgatagac gcagatagtc
2941gcccaaagtt ccgtgagttg atcatcgaat tctccaaaat ggcccgagac ccccagcgct
3001accttgtcat tcagggggat gaaagaatgc atttgccaag tcctacagac tccaacttct
3061accgtgccct gatggatgaa gaagacatgg acgacgtggt ggatgccgac gagtacctca
3121tcccacagca gggcttcttc agcagcccct ccacgtcacg gactcccctc ctgagctctc
3181tgagtgcaac cagcaacaat tccaccgtgg cttgcattga tagaaatggg ctgcaaagct
3241gtcccatcaa ggaagacagc ttcttgcagc gatacagctc agaccccaca ggcgccttga
3301ctgaggacag catagacgac accttcctcc cagtgcctga atacataaac cagtccgttc
3361ccaaaaggcc cgctggctct gtgcagaatc ctgtctatca caatcagcct ctgaaccccg
3421cgcccagcag agacccacac taccaggacc cccacagcac tgcagtgggc aaccccgagt
3481atctcaacac tgtccagccc acctgtgtca acagcacatt cgacagccct gcccactggg
3541cccagaaagg cagccaccaa attagcctgg acaaccctga ctaccagcag gacttctttc
3601ccaaggaagc caagccaaat ggcatcttta agggctccac agctgaaaat gcagaatacc
3661taagggtcgc gccacaaagc agtgaattta ttggagcatt gccaactttc ttgtacaaag
3721ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaac

Download FASTA (ORF) (Full)

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