Construct: ORF ccsbBroadEn_14743
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018880.1_s300c1, BRDN0000458176
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PFKFB2 (5208)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5208 | PFKFB2 | 6-phosphofructo-2-kinase/fr... | NM_006212.2 | 98.6% | 48.8% | (many diffs) |
| 2 | human | 5208 | PFKFB2 | 6-phosphofructo-2-kinase/fr... | XM_024447654.1 | 98.6% | 48.8% | (many diffs) |
| 3 | human | 5208 | PFKFB2 | 6-phosphofructo-2-kinase/fr... | XM_024447655.1 | 98.6% | 48.8% | (many diffs) |
| 4 | human | 5208 | PFKFB2 | 6-phosphofructo-2-kinase/fr... | XM_024447656.1 | 92.1% | 42.3% | (many diffs) |
| 5 | human | 5208 | PFKFB2 | 6-phosphofructo-2-kinase/fr... | NM_001018053.2 | 90.4% | 52.3% | (many diffs) |
| 6 | human | 5208 | PFKFB2 | 6-phosphofructo-2-kinase/fr... | XM_024447657.1 | 90.4% | 52.3% | (many diffs) |
| 7 | human | 5208 | PFKFB2 | 6-phosphofructo-2-kinase/fr... | XM_005273162.3 | 51% | 13.2% | (many diffs) |
| 8 | mouse | 18640 | Pfkfb2 | 6-phosphofructo-2-kinase/fr... | NM_008825.4 | 86.4% | 43.5% | (many diffs) |
| 9 | mouse | 18640 | Pfkfb2 | 6-phosphofructo-2-kinase/fr... | NM_001162416.1 | 82.7% | 45.4% | (many diffs) |
| 10 | mouse | 18640 | Pfkfb2 | 6-phosphofructo-2-kinase/fr... | NM_001162415.1 | 80.6% | 47.6% | (many diffs) |
| 11 | mouse | 18640 | Pfkfb2 | 6-phosphofructo-2-kinase/fr... | NR_027859.1 | 28.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 98
- ORF end:
- 908
- ORF length:
- 810
- Sequence:
-
1 CATGATTTTA TTTTGACTGA TAGTGACCTG TTCGTTGCAA CAAATTGATG AGCAATGCTT 61 TTTTATAATG CCAACTTTGT ACAAAAAAGT TGGCACCATG TCTGGGGCAT CTTCCTCAGA 121 ACAGAACAAC AACAGCTATG AAACCAAAAC CCCAAATCTT CGAATGTCAG AGAAGAAATG 181 TTCATGGGCC TCCTACATGA CCAACTCCCC GACTCTGATC GTTATGATTG GTTTGCCAGC 241 CCGGGGTAAA ACCTACGTGT CCAAGAAACT AACACGCTAC CTCAACTGGA TTGGAGTCCC 301 CACCAAAGTG TTTAATCTTG GGGTGTATCG GCGTGAAGCA GTCAAGTCCT ATAAGTCCTA 361 CGACTTCTTT CGGCATGACA ATGAGGAGGC CATGAAGATC CGCAAACAGT GTGCTCTGGT 421 GGCGCTGGAA GATGTTAAGG CGTATCTCAC TGAGGAGAAT GGTCAGATTG CGGTGTTTGA 481 TGCCACCAAT ACAACCCGGG AGAGGAGGGA CATGATTTTG AACTTTGCTG AACAGAATTC 541 CTTCAAGGTA TTCTTTGTGG AATCCGTCTG TGATGATCCT GATGTCATTG CTGCCAATAT 601 TCTGGAGGTT AAGGTATCAA GCCCTGACTA TCCTGAAAGG AATAGAGAGA ACGTGATGGA 661 GGACTTCCTG AAGAGAATTG AATGCTACAA AGTTACCTAC CGACCTCTTG ACCCAGACAA 721 CTATGACAAG GATCTTTCTT TCATCAAAGT GATAAACGTG GGCCAGCGAT TTTTAGTCAA 781 CAGAGTCCAG GACTACATCC AGAGCCAGAT AGTCTACTAC CTCATGAANA TCCACGTCCA 841 NCNCTCGCAC CATTTACCTT TNNCGGCATG GANNAAGCGA GTTCNATCTC TTGGGNAANA 901 TNGGGGGTGA CTCTGGCCTC CTCGGTGCGG GGNAAGCAGT TTGCCCAAGC TCTAAGGAAA 961 TTTCTGAGGG AACAGGAAAT AACAGACCTC AAAGTGTGGA CAAGCCAGTG NAAGAGGACC 1021 ATACAGACTG CTGAATCTCT CGGGGTGCCC TATGAGCAGT GGAAGATTCT GAATGAGATT 1081 GATGCTGGTG TGTGTGAAGA GATGACCTAT GCAGAGATTG AGAAACGGTA CCCAGAAGAG 1141 TTTGCACTTC GAGATCAAGA GAAGTATCTG TATCGATATC CTGGTGGGGA GTCATACCAG 1201 GACCTGGTGC AGCGGCTGGA GCCTGTCATC ATGGAGCTGG AACGTCAGGG CAATGTCCTC 1261 GTCATCTCCC ACCAGGCTGT CATGCGCTGC CTCCTGGCCT ACTTCTTGGA TAAGGGCGCA 1321 GATGAGCTAC CATACTTGAG ATGCCCTCTC CATACCATCT TCAAACTTAC TCCTGTGGCC 1381 TATGGGTGCA AAGTGGAAAC AATTAAACTT AACGTGGAGG CTGTGAACAC GCACCGTGAC 1441 AAGCCAACTA ACAACTTCCC CAAGAACCAA ACCCCTGTAA GGATGAGAAG GAACAGCTTT 1501 ACGCCTCTGT CCAGTTCGAA TACAATAAGG CGTCCAAGAA ATTACAGTGT TGGGAGCCGG 1561 CCCCTCAAGC CCCTCAGCCC TCTCCGTGCC CAGGACATGC AAGAAGGGGC CGACTTGCCA 1621 ACTTTCTTGT ACAAAGTTGG CATTATAAGA AAGCATTGCT TATCAATTTG TTGCAACGAA 1681 CAGGTCACTA TCAGTCAAAA TAAAATCATT ATTGCCATCC CAGCCTGATT TC