Construct: ORF ccsbBroadEn_14755
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018821.1_s300c1, BRDN0000458117
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PHKG1 (5260)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5260 | PHKG1 | phosphorylase kinase cataly... | NM_006213.4 | 99.6% | 34.8% | (many diffs) |
| 2 | human | 5260 | PHKG1 | phosphorylase kinase cataly... | NM_001258460.1 | 97.3% | 32.5% | (many diffs) |
| 3 | human | 5260 | PHKG1 | phosphorylase kinase cataly... | NM_001258459.1 | 92% | 32.2% | (many diffs) |
| 4 | human | 5260 | PHKG1 | phosphorylase kinase cataly... | XM_017012324.2 | 89.2% | 31.2% | (many diffs) |
| 5 | human | 5260 | PHKG1 | phosphorylase kinase cataly... | XM_005271772.5 | 79.5% | 14.7% | (many diffs) |
| 6 | human | 5260 | PHKG1 | phosphorylase kinase cataly... | XM_017012325.2 | 76.8% | 9% | (many diffs) |
| 7 | human | 5260 | PHKG1 | phosphorylase kinase cataly... | XM_017012326.2 | 71.2% | 13.1% | (many diffs) |
| 8 | human | 5260 | PHKG1 | phosphorylase kinase cataly... | XM_017012327.2 | 64.7% | 6.7% | (many diffs) |
| 9 | human | 5260 | PHKG1 | phosphorylase kinase cataly... | NR_047689.1 | 51.7% | (many diffs) | |
| 10 | mouse | 18682 | Phkg1 | phosphorylase kinase gamma 1 | NM_011079.3 | 86.9% | 32.4% | (many diffs) |
| 11 | mouse | 18682 | Phkg1 | phosphorylase kinase gamma 1 | XM_011240871.2 | 63.1% | 7.2% | (many diffs) |
| 12 | mouse | 18682 | Phkg1 | phosphorylase kinase gamma 1 | NR_145486.1 | 34.4% | (many diffs) | |
| 13 | mouse | 18682 | Phkg1 | phosphorylase kinase gamma 1 | NR_145487.1 | 34.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 95
- ORF end:
- 572
- ORF length:
- 477
- Sequence:
-
1 GGATTTTTTT TGACTGATAG TGACCTGTTC GTTGCAACAA ATTGATGAGC AATGCTTTTT 61 TATAATGCCA ACTTTGTACA AAAAAGTTGG CACCATGACC CGGGACGAGG CACTGCCGGA 121 CTCTCATTCT GCACAGGACT TCTATGAGAA TTATGAGCCC AAAGAGATCC TGGGCAGGGG 181 CGTTAGCAGT GTGGTCAGGC GATGCATCCA CAAGCCCACG AGCCAGGAGT ACGCCGTGAA 241 GGTCATCGAC GTCACCGGTG GAGGCAGCTT CAGCCCGGAG GAGGTGCGGG AGCTGCGAGA 301 AGCCACGCTG AAGGAGGTGG ACATCCTGCG CAAGGTCTCA GGGCACCCCA ACATCATACA 361 GCTGAAGGAC ACTTATGAGA CCAACACTTT CTTCTTCTTG GTGTTTGACC TGATGAAGAG 421 AGGGGAGCTC TTTGACTACC TCACTGAGAA GGTCACCTTG AGTGAGAAGG AAACCAGAAA 481 ANATCATGCG AGCTCTGCTG GAGGTGATCT GCACCTTGCA CAAACTCAAC ATCGTGCACC 541 GGGACCTGAA GCCCGAGAAC ATTCTCTTGG ATGACAACAT GAACATCAAG CTCACAGACT 601 TTGGCTTTTC CTGCCAGCTG GAGCCGGGAG AGAGGCTGCG AGAGGTCTGT GGGACCCCCA 661 GTTACCTGGC CCCTGAGATT ATCGAGTGCT CCATGAATGA GGACCACCCG GGCTACGGGA 721 AAGAGGTGGA CATGTGGAGC ACTGGCGTCA TCATGTACAC GCTGCTGGCC GGCTCCCCGC 781 CCTTCTGGCA CCGGAAGCAG ATGCTGATGC TGAGGATGAT CATGAGCGGC AACTACCAGT 841 TTGGCTCGCC CGAGTGGGAT GATTACTCGG ACACCGTGAA GGACCTGGTT CTCCCGATTC 901 CTGGTGGTGC AACCCCAGAA CCGCTACACA GCGGAAGAGG CCTTGGCACA CCCCTTCTTC 961 CAGCAGTACT TGGTGGAGGA AGTGCGGCAC TTCAGCCCCC GGGGGAAGTT CAAGGTGATC 1021 GCTCTGACCG TGCTGGCTTC AGTGCGGATC TACTACCAGT ACCGCCGGGT GAAGCCTGTG 1081 ACCCGGGAGA TCGTCATCCG AGACCCCTAT GCCCTCCGGC CTCTGCGCCG GCTCATCGAC 1141 GCCTACGCTT TCCGAATCTA TGGCCACTGG GTGAAGAAGG GGCAGCAGCA GAACCGGGCA 1201 GCCCTTTTCG AGAACACACC CAAGGCCGTG CTCCTCTCCC TGGCCGAGGA GGACTACTTG 1261 CCAACTTTCT TGTACAAAGT TGGCATTATA AGAAAGCATT GCTTATCAAT TTGTTGCAAC 1321 GAACAGGTCA CTATCAGTCA AAATAAAATC ATTATTGCCA TCCAGCGCTC TACA