Transcript: Mouse XM_011240871.2

PREDICTED: Mus musculus phosphorylase kinase gamma 1 (Phkg1), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Phkg1 (18682)
Length:
1564
CDS:
437..1285

Additional Resources:

NCBI RefSeq record:
XM_011240871.2
NBCI Gene record:
Phkg1 (18682)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_011240871.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000024210 CAAACCCACATGCCAGGAATA pLKO.1 240 5UTR 100% 10.800 8.640 N Phkg1 n/a
2 TRCN0000361405 GATGATCATGGACGGCAAATA pLKO_005 838 CDS 100% 13.200 9.240 N Phkg1 n/a
3 TRCN0000024209 CCGGAAGCAAATGCTGATGTT pLKO.1 814 CDS 100% 4.950 3.465 N Phkg1 n/a
4 TRCN0000024212 CTTGGTATTTGATCTGATGAA pLKO.1 421 5UTR 100% 4.950 3.465 N Phkg1 n/a
5 TRCN0000024213 GCTGGCATCAGTAAAGATCTA pLKO.1 1054 CDS 100% 4.950 3.465 N Phkg1 n/a
6 TRCN0000024211 CCTTTCTTTCAGGAGTACGTA pLKO.1 974 CDS 100% 3.000 2.100 N Phkg1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011240871.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000491471 AAACGAACCACGTCGCTTGAGTTC pLX_317 19.4% 63.2% 67.8% V5 (many diffs) n/a
2 TRCN0000489075 CTGCCCGAGCCCAGCAATAAATTT pLX_317 32.1% 63.2% 68% V5 (not translated due to prior stop codon) (many diffs) n/a
3 TRCN0000473797 ACCTCCCCATTAAGACGTTATGAT pLX_317 36.8% 63.1% 7.2% V5 (not translated due to prior stop codon) (many diffs) n/a
4 ccsbBroadEn_14755 pDONR223 100% 63.1% 7.2% None (many diffs) n/a
5 ccsbBroad304_14755 pLX_304 0% 63.1% 7.2% V5 (not translated due to prior stop codon) (many diffs) n/a
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