Transcript: Human XM_017012327.2

PREDICTED: Homo sapiens phosphorylase kinase catalytic subunit gamma 1 (PHKG1), transcript variant X4, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
PHKG1 (5260)
Length:
1478
CDS:
143..1123

Additional Resources:

NCBI RefSeq record:
XM_017012327.2
NBCI Gene record:
PHKG1 (5260)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001147981 TCCGAGTAATCATCCCACTC pXPR_003 GGG 576 59% 6 0.806 PHKG1 PHKG1 76357
2 BRDN0001147570 TCAAGGTGACCTTCTCAGTG pXPR_003 AGG 32 3% 2 0.5671 PHKG1 PHKG1 76355
3 BRDN0001144981 TCCATGAATGAGGACCACCC pXPR_003 GGG 428 44% 5 0.5042 PHKG1 PHKG1 76356
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017012327.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000006205 GCCTACGCTTTCCGAATCTAT pLKO.1 1004 CDS 100% 5.625 7.875 N PHKG1 n/a
2 TRCN0000197280 GATGATCATGAGCGGCAACTA pLKO.1 679 CDS 100% 4.950 6.930 N PHKG1 n/a
3 TRCN0000219704 TGCGGATCTACTACCAGTACC pLKO.1 906 CDS 100% 4.050 5.670 N PHKG1 n/a
4 TRCN0000199306 CCTGAGATTATCGAGTGCTCC pLKO.1 536 CDS 100% 2.160 3.024 N PHKG1 n/a
5 TRCN0000219703 TGATCTGCACCTTGCACAAAC pLKO.1 234 CDS 100% 10.800 7.560 N PHKG1 n/a
6 TRCN0000380988 TTATCGAGTGCTCCATGAATG pLKO_005 543 CDS 100% 10.800 7.560 N PHKG1 n/a
7 TRCN0000006201 GCCACCATCTCCATTTCTCTT pLKO.1 1408 3UTR 100% 4.950 3.465 N PHKG1 n/a
8 TRCN0000006203 GCTGATGCTGAGGATGATCAT pLKO.1 667 CDS 100% 4.950 3.465 N PHKG1 n/a
9 TRCN0000165534 GAGACAGGGTTTCACCATGTT pLKO.1 374 CDS 100% 4.950 2.475 Y n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017012327.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000489075 CTGCCCGAGCCCAGCAATAAATTT pLX_317 32.1% 65% 65% V5 (not translated due to prior stop codon) 0_1ins318;228_362del n/a
2 TRCN0000491471 AAACGAACCACGTCGCTTGAGTTC pLX_317 19.4% 64.9% 64.8% V5 0_1ins318;228_362del;978_979insG n/a
3 TRCN0000473797 ACCTCCCCATTAAGACGTTATGAT pLX_317 36.8% 64.8% 6.7% V5 (not translated due to prior stop codon) (many diffs) n/a
4 ccsbBroadEn_14755 pDONR223 100% 64.7% 6.7% None (many diffs) n/a
5 ccsbBroad304_14755 pLX_304 0% 64.7% 6.7% V5 (not translated due to prior stop codon) (many diffs) n/a
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