Transcript: Mouse NM_011079.3

Mus musculus phosphorylase kinase gamma 1 (Phkg1), mRNA.

Source:
NCBI, updated 2017-06-10
Taxon:
Mus musculus (mouse)
Gene:
Phkg1 (18682)
Length:
2369
CDS:
193..1359

Additional Resources:

NCBI RefSeq record:
NM_011079.3
NBCI Gene record:
Phkg1 (18682)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_011079.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000024210 CAAACCCACATGCCAGGAATA pLKO.1 309 CDS 100% 10.800 8.640 N Phkg1 n/a
2 TRCN0000361405 GATGATCATGGACGGCAAATA pLKO_005 912 CDS 100% 13.200 9.240 N Phkg1 n/a
3 TRCN0000368780 TCAACCTCTGGTACAGCTAAA pLKO_005 1667 3UTR 100% 10.800 7.560 N Phkg1 n/a
4 TRCN0000024209 CCGGAAGCAAATGCTGATGTT pLKO.1 888 CDS 100% 4.950 3.465 N Phkg1 n/a
5 TRCN0000024212 CTTGGTATTTGATCTGATGAA pLKO.1 495 CDS 100% 4.950 3.465 N Phkg1 n/a
6 TRCN0000024213 GCTGGCATCAGTAAAGATCTA pLKO.1 1128 CDS 100% 4.950 3.465 N Phkg1 n/a
7 TRCN0000024211 CCTTTCTTTCAGGAGTACGTA pLKO.1 1048 CDS 100% 3.000 2.100 N Phkg1 n/a
8 TRCN0000379650 ATACAGCTGAAGGACACTTAT pLKO_005 454 CDS 100% 13.200 9.240 N PHKG1 n/a
9 TRCN0000198429 GCTACATGAAACCCTATCTTA pLKO.1 2120 3UTR 100% 5.625 2.813 Y Angel2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_011079.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000491471 AAACGAACCACGTCGCTTGAGTTC pLX_317 19.4% 87.1% 93% V5 (many diffs) n/a
2 TRCN0000489075 CTGCCCGAGCCCAGCAATAAATTT pLX_317 32.1% 87.1% 93.2% V5 (not translated due to prior stop codon) (many diffs) n/a
3 TRCN0000473797 ACCTCCCCATTAAGACGTTATGAT pLX_317 36.8% 87% 32.4% V5 (not translated due to prior stop codon) (many diffs) n/a
4 ccsbBroadEn_14755 pDONR223 100% 86.9% 32.4% None (many diffs) n/a
5 ccsbBroad304_14755 pLX_304 0% 86.9% 32.4% V5 (not translated due to prior stop codon) (many diffs) n/a
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