Construct: ORF ccsbBroadEn_14764
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018977.1_s300c1, BRDN0000458273
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PI4KA (5297)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5297 | PI4KA | phosphatidylinositol 4-kina... | XM_017028830.1 | 57.5% | 15.1% | (many diffs) |
| 2 | human | 728233 | PI4KAP1 | phosphatidylinositol 4-kina... | NR_003563.1 | 52.9% | (many diffs) | |
| 3 | human | 375133 | PI4KAP2 | phosphatidylinositol 4-kina... | NR_003700.1 | 50.4% | (many diffs) | |
| 4 | human | 5297 | PI4KA | phosphatidylinositol 4-kina... | NM_001362862.1 | 40.8% | 10.7% | (many diffs) |
| 5 | human | 5297 | PI4KA | phosphatidylinositol 4-kina... | NM_001362863.1 | 40.6% | 10.7% | (many diffs) |
| 6 | human | 5297 | PI4KA | phosphatidylinositol 4-kina... | NM_058004.4 | 40.2% | 10.6% | (many diffs) |
| 7 | human | 5297 | PI4KA | phosphatidylinositol 4-kina... | XR_937868.1 | 35.8% | (many diffs) | |
| 8 | human | 5297 | PI4KA | phosphatidylinositol 4-kina... | XM_005261635.1 | 26.9% | 10.4% | (many diffs) |
| 9 | mouse | 224020 | Pi4ka | phosphatidylinositol 4-kina... | XM_006522037.2 | 64.6% | 18.6% | (many diffs) |
| 10 | mouse | 224020 | Pi4ka | phosphatidylinositol 4-kina... | NM_001001983.2 | 37% | 10.7% | (many diffs) |
| 11 | mouse | 224020 | Pi4ka | phosphatidylinositol 4-kina... | XM_006522034.1 | 36% | 10.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 95
- ORF end:
- 806
- ORF length:
- 711
- Sequence:
-
1 AATGATTTTA TTTTGACTGA TAGTGACCTG TTCGTTGCAA CAAATTGATG AGCAATGCTT 61 TTTTATAATG CCAACTTTGT ACAAAAAAGT TGGCATGCGG GAGATGGCAG GGGCCTGGCA 121 CATGACGGTG GAGCAGAAAT TTGGCCTGTT TTCTGCTGAG ATAAAGGAAG CAGACCCCCT 181 GGCTGCCTCG GAAGCAAGTC AACCCAAACC CTGTCCCCCC GAAGTGACCC CCCACTACAT 241 CTGGATCGAC TTCCTGGTGC AGCGGTTTGA GATCGCCAAG TACTGCAGCT CTGACCAAGT 301 GGAGATCTTC TCCAGCCTGC TGCAGCGCTC CATGTCCCTG AACATCGGCG GGGCCAAGGG 361 GAGCATGAAC CGGCACGTGG CGGCCATCGG GCCCCGCTTC AAGCTGCTGA CCCTGGGGCT 421 GTCCCTCCTG CATGCCGATG TGGTTCCAAA TGCAACCATC CGCAATGTGC TTCGCGAGAA 481 GATCTACTCC ACTGCCTTTG ACTACTTCAG CTGTCCCCCA AAGTTCCCTA CTCAAGGAGA 541 GAAGCGGCTG CGTGAAGACA TAAGCATCAT GATTAAATTT TGGACCGCCA TGTTCTCAGA 601 TAAGAAGTAC CTGACCGCCA GCCAGCTTGT TCCCCCAGAT AATCAGGACA CCCGGAGCAA 661 CCTGGACATA ACTGTCGGCT CTCGGCAACA AGCCACCCAA GGCTGGATCA ACACATACCC 721 CCTGTCCAGC GGCATGTCCA CCATCTCCAA GAAATCAGCA TGTCTAAGAA AACCAACCGG 781 GGCTCCCAGC TGCACAAATA CTACATGAAG CGCAGACGCT GCTGCTGTCC CTGCTGGCCA 841 CTGAGATCGA GCGTCTCATC ACATGGTACA CCCGCTGTCA GCCCCGGAAC TGGAACTAGA 901 CAAGGCCGAA AAAGAACAGC CGTGGCCACT GGGAGATTCT Aagtacatca gcctgagtga 961 gaagcagtgg aaggacaacg tgaacctcgc ctggagcatc tctccctacc tagccgtgca 1021 gctgcctgcc aggtttaaga acacagaagc cattgggaac gaagtgaccc gtctcgttcg 1081 gttggacccg ggagccgtta gtgatgtgcc tgaagcaatc aagttcctgg tcacctggca 1141 caccatcgac gccgatgctc cagagctcag ccatgtgctg tgctgggcgc ccacggaccc 1201 acccacaggc ctctcctact tctccagcat gtacccgccg caccctctca cggcgcagta 1261 cggggtgaaa gtcctgcggt ccttccctcc ggacgccatc ctcttctaca tcccccagat 1321 tgtgcaggcc ctcaggtacg acaagatggg ctatgtgcgg gagtatattc tgtgggcagc 1381 gtctaaatcc cagcttctgg cacaccagtt catctggaac atgaagacta acatttatct 1441 agatgaagag ggccaccaga aagaccctga catcggcgac ctcctggatc agttggtaga 1501 ggagatcaca ggctccttgt ccggcccagc gaaggacttt taccagcggg agtttgattt 1561 ctttaacaag atcaccaacg tgtcggctat catcaagccc taccctaaag gcgacgagag 1621 aaagaaggct tgtctgtcgg ccctgtctga agtgaaggtg cagccgggct gctacctgcc 1681 cagcaaccct gaggccattg tgctggacat cgactacaag tctgggaccc cgatgcagag 1741 tgctgcaaaa gccccatatc tggccaagtt caaggtgaag cgatgtggag ttagtgaact 1801 tgaaaaagaa ggtctgcggt gccgctcaga ctccgaggat gAGTCAGCAA GCAGAAGCCC 1861 GACGCCAGAA AGATTTCTGG CAGGCAGCAT CTTCAAAGGT GGGAGACGAC TGCCGGCAAG 1921 ACATGCTGGC CCTGCAGATC ATCGACCTCN TCAAAGAACA TCTCCCAGGC TGTCGGCCTG 1981 GACCTCTTTG TTTTTCCCNT ACCGCGTGGT GGCCACTGCC CCTGGGTGCG GGGTGATCGA 2041 GTGCATCCCC GACTGCACCT CCCGGGACCA GCTGGGCCGC CAGACAGACT TCGGCATGTA 2101 CGACTACTTC ACACGCCAGT ACGGGGATGA GTCCACTCTG GCCTTCCAGC AGGCCCGCTA 2161 CAACTTCATC CGAAGCATGG CCGCCTACAG CCTCCTGCTG TTCCTGCTGC AGATCAAGGA 2221 CAGACACAAC GGCAACATTA TGCTGGACAA GAAGGGTCAT ATCATCCACA TCGACTTTGG 2281 CTTCATGTTT GAAAGCTCGC CGGGCGGCAA TCTCGGCTGG GAACCCGACA TCAAGCTGAC 2341 GGATGAGATG GTGATGATCA TGGGGGGCAA GATGGAGGCC ACACCCTTCA AGTGGTTCAT 2401 GGAGATGTGT GTCCGAGGCT ACCTGGCTGT GCGGCCCTAC ATGGACGCGG TCGTCTCCCT 2461 GGTCACTCTC ATGTTGGACA CGGGCCTGCC CTGTTTTCGC GGCCAGACAA TCAAGCTCTT 2521 GAAGCACAGG TTTAGCCCCA ACATGACTGA GCGCGAGGCT GCAAATTTCA TCATGAAGGT 2581 CATCCAGAGC TGCTTCCTCA GCAACAGGAG CCGGACCTAC GACATGATCC AGTACTATCA 2641 GAATGACATC CCCTACTTGC CAACTTTCTT GTACAAAGTT GGCATTATAA GAAAGCATTG 2701 CTTATCAATT TGTTGCAACG AACAGGTCAC TATCAGTCAA AATAAAATCA TTATTGCCCA 2761 TCCTCAG