Construct: ORF ccsbBroadEn_14807
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018842.1_s300c1, BRDN0000458138
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- MAP2K1 (5604)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5604 | MAP2K1 | mitogen-activated protein k... | NM_002755.3 | 99.8% | 36.3% | 413_414insG;603_604insG |
| 2 | human | 5604 | MAP2K1 | mitogen-activated protein k... | XM_011521783.3 | 93.5% | 29.5% | (many diffs) |
| 3 | human | 5604 | MAP2K1 | mitogen-activated protein k... | XM_017022411.2 | 93.2% | 39.2% | 413_414insG;438_439ins78;525_526insG |
| 4 | human | 5604 | MAP2K1 | mitogen-activated protein k... | XM_017022412.1 | 86.9% | 31.8% | (many diffs) |
| 5 | human | 5604 | MAP2K1 | mitogen-activated protein k... | XM_017022413.1 | 53.3% | 1.6% | (many diffs) |
| 6 | mouse | 26395 | Map2k1 | mitogen-activated protein k... | NM_008927.3 | 90.5% | 36.3% | (many diffs) |
| 7 | mouse | 26395 | Map2k1 | mitogen-activated protein k... | XM_006511196.3 | 84.7% | 29.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 97
- ORF end:
- 583
- ORF length:
- 486
- Sequence:
-
1 TAGATTTTAT TTTGACTGAT AGTGACCTGT TCGTTGCAAC AAATTGATGA GCAATGCTTT 61 TTTATAATGC CAACTTTGTA CAAAAAAGTT GGCACCATGC CCAAGAAGAA GCCGACGCCC 121 ATCCAGCTGA ACCCGGCCCC CGACGGCTCT GCAGTTAACG GGACCAGCTC TGCGGAGACC 181 AACTTGGAGG CCTTGCAGAA GAAGCTGGAG GAGCTAGAGC TTGATGAGCA GCAGCGAAAG 241 CGCCTTGAGG CCTTTCTTAC CCAGAAGCAG AAGGTGGGAG AACTGAAGGA TGACGACTTT 301 GAGAAGATCA GTGAGCTGGG GGCTGGCAAT GGCGGTGTGG TGTTCAAGGT CTCCCACAAG 361 CCTTCTGGCC TGGTCATGGC CAGAAAGCTA ATTCATCTGG AGATCAAACC CGCAATCCGG 421 AACCAGATCA TAAGGGAGCT GCAGGTTCTG CATGAGTGCA ACTCTCCGTA CATCGTGGGC 481 TTCTATGGTG CGTTCTACAG CGATGGCGAG GATCAGTATC TGCATGGAGC ACATGGATGG 541 AGGTTCTCTG GATCAAGTCC TGAAGAAAGC TGGAAGAATT CCTGAACAAA TTTTAGGAAA 601 AGTTAGCATT GCTGTAATAA AAGGCCTGAC ATATCTGAGG GAGAAGCACA AGATCATGCA 661 CAGAGATGTC AAGCCCTCCA ACATCCTAGT CAACTCCCGT GGGGGAGATC AAGCTCTGTG 721 ACTTTGGGGT CAGCGGGCAG CTCATCGACT CCATGGCCAA CTCCTTCGTG GGCACAAGGT 781 CCTACATGTC GCCAGAAAGA CTCCAGGGGA CTCATTACTC TGTGCAGTCA GACATCTGGA 841 GCATGGGACT GTCTCTGGTA GAGATGGCGG TTGGGAGGTA TCCCATCCCT CCTCCAGATG 901 CCAAGGAGCT GGAGCTGATG TTTGGGTGCC AGGTGGAAGG AGATGCGGCT GAGACCCCAC 961 CCAGGCCAAG GACCCCCGGG AGGCCCCTTA GCTCATACGG AATGGACAGC CGACCTCCCA 1021 TGGCAATTTT TGAGTTGTTG GATTACATAG TCAACGAGCC TCCTCCAAAA CTGCCCAGTG 1081 GAGTGTTCAG TCTGGAATTT CAAGATTTTG TGAATAAATG CTTAATAAAA AACCCCGCAG 1141 AGAGAGCAGA TTTGAAGCAA CTCATGGTTC ATGCTTTTAT CAAGAGATCT GATGCTGAGG 1201 AAGTGGATTT TGCAGGTTGG CTCTGCTCCA CCATCGGCCT TAACCAGCCC AGCACACCAA 1261 CCCATGCTGC TGGCGTCTTG CCAACTTTCT TGTACAAAGT TGGCATTATA AGAAAGCATT 1321 GCTTATCAAT TTGTTGCAAC GAACAGGTCA CTATCAGTCA AAATAAAATC ATTATTGCCA 1381 TCCAGCTGAT TC