Construct: ORF ccsbBroadEn_14960

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011814.2_s300c1, BRDN0000458445
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PAK4 (10298)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10298 PAK4 p21 (RAC1) activated kinase 4 NM_001014831.3 100% 100%
2 human 10298 PAK4 p21 (RAC1) activated kinase 4 NM_001014832.2 100% 100%
3 human 10298 PAK4 p21 (RAC1) activated kinase 4 NM_005884.4 100% 100%
4 human 10298 PAK4 p21 (RAC1) activated kinase 4 XM_011526316.1 100% 100%
5 human 10298 PAK4 p21 (RAC1) activated kinase 4 XM_011526317.2 100% 100%
6 human 10298 PAK4 p21 (RAC1) activated kinase 4 XM_011526318.2 100% 100%
7 human 10298 PAK4 p21 (RAC1) activated kinase 4 XM_011526319.2 100% 100%
8 human 10298 PAK4 p21 (RAC1) activated kinase 4 NM_001014834.3 74.1% 74.1% 202_203ins459
9 human 10298 PAK4 p21 (RAC1) activated kinase 4 NM_001014835.2 74.1% 74.1% 202_203ins459
10 human 10298 PAK4 p21 (RAC1) activated kinase 4 XM_011526320.1 74.1% 74.1% 202_203ins459
11 human 10298 PAK4 p21 (RAC1) activated kinase 4 XM_024451314.1 74.1% 74.1% 202_203ins459
12 mouse 70584 Pak4 p21 protein (Cdc42/Rac)-act... NM_027470.3 86.2% 92.5% (many diffs)
13 mouse 70584 Pak4 p21 protein (Cdc42/Rac)-act... XM_017312296.1 86.2% 92.5% (many diffs)
14 mouse 70584 Pak4 p21 protein (Cdc42/Rac)-act... XM_017312297.1 86.2% 92.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1839
ORF length:
1773
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag
61ttggcatgtt tgggaagagg aagaagcggg tggagatctc cgcgccgtcc aacttcgagc
121accgcgtgca cacgggcttc gaccagcacg agcagaagtt cacggggctg ccccgccagt
181ggcagagcct gatcgaggag tcggctcgcc ggcccaagcc cctcgtcgac cccgcctgca
241tcacctccat ccagcccggg gcccccaaga ccatcgtgcg gggcagcaaa ggtgccaaag
301atggggccct cacgctgctg ctggacgagt ttgagaacat gtcggtgaca cgctccaact
361ccctgcggag agacagcccg ccgccgcccg cccgtgcccg ccaggaaaat gggatgccag
421aggagccggc caccacggcc agagggggcc cagggaaggc aggcagccga ggccggttcg
481ccggtcacag cgaggcgggt ggcggcagtg gtgacaggcg acgggcgggg ccagagaaga
541ggcccaagtc ttccagggag ggctcagggg gtccccagga gtcctcccgg gacaaacgcc
601ccctctccgg gcctgatgtc ggcacccccc agcctgctgg tctggccagt ggggcgaaac
661tggcagctgg ccggcccttt aacacctacc cgagggctga cacggaccac ccatcccggg
721gtgcccaggg ggagcctcat gacgtggccc ctaacgggcc atcagcgggg ggcctggcca
781tcccccagtc ctcctcctcc tcctcccggc ctcccacccg agcccgaggt gcccccagcc
841ctggagtgct gggaccccac gcctcagagc cccagctggc ccctccagcc tgcacccccg
901ccgcccctgc tgttcctggg ccccctggcc cccgctcacc acagcgggag ccacagcgag
961tatcccatga gcagttccgg gctgccctgc agctggtggt ggacccaggc gacccccgct
1021cctacctgga caacttcatc aagattggcg agggctccac gggcatcgtg tgcatcgcca
1081ccgtgcgcag ctcgggcaag ctggtggccg tcaagaagat ggacctgcgc aagcagcaga
1141ggcgcgagct gctcttcaac gaggtggtaa tcatgaggga ctaccagcac gagaatgtgg
1201tggagatgta caacagctac ctggtggggg acgagctctg ggtggtcatg gagttcctgg
1261aaggaggcgc cctcaccgac atcgtcaccc acaccaggat gaacgaggag cagatcgcgg
1321ccgtgtgcct tgcagtgctg caggccctgt cggtgctcca cgcccagggc gtcatccacc
1381gggacatcaa gagcgactcg atcctgctga cccatgatgg cagggtgaag ctgtcagact
1441ttgggttctg cgcccaggtg agcaaggaag tgccccgaag gaagtcgctg gtcggcacgc
1501cctactggat ggccccagag ctcatctccc gccttcccta cgggccagag gtagacatct
1561ggtcgctggg gataatggtg attgagatgg tggacggaga gcccccctac ttcaacgagc
1621cacccctcaa agccatgaag atgattcggg acaacctgcc accccgactg aagaacctgc
1681acaaggtgtc gccatccctg aagggcttcc tggaccgcct gctggtgcga gaccctgccc
1741agcgggccac ggcagccgag ctgctgaagc acccattcct ggccaaggca gggccgcctg
1801ccagcatcgt gcccctcatg cgccagaacc gcaccagatg cccaactttc ttgtacaaag
1861ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaac

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