Transcript: Human NM_001014832.2

Homo sapiens p21 (RAC1) activated kinase 4 (PAK4), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-06-04
Taxon:
Homo sapiens (human)
Gene:
PAK4 (10298)
Length:
6169
CDS:
157..1932

Additional Resources:

NCBI RefSeq record:
NM_001014832.2
NBCI Gene record:
PAK4 (10298)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001147629 GGACGAGTTTGAGAACATGT pXPR_003 CGG 274 15% 3 0.8563 PAK4 PAK4 77573
2 BRDN0001146148 CCAGTGGCAGAGCCTGATCG pXPR_003 AGG 127 7% 2 0.3751 PAK4 PAK4 77574
3 BRDN0001148325 TGCTCATGGGATACTCGCTG pXPR_003 TGG 891 50% 4 -0.1152 PAK4 PAK4 77576
4 BRDN0001145608 CCGGTTCGCCGGTCACAGCG pXPR_003 AGG 424 24% 3 -0.4434 PAK4 PAK4 77575
Download CSV

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001014832.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000272510 ACTAAGAGGTGAACATGTATG pLKO_005 2267 3UTR 100% 10.800 15.120 N PAK4 n/a
2 TRCN0000195628 CGATCATGAATGTCCGAAGAG pLKO.1 2420 3UTR 100% 4.050 5.670 N PAK4 n/a
3 TRCN0000010200 CGACCAGCACGAGCAGAAGTT pLKO.1 231 CDS 100% 1.650 2.310 N PAK4 n/a
4 TRCN0000272579 CGACCAGCACGAGCAGAAGTT pLKO_005 231 CDS 100% 1.650 2.310 N PAK4 n/a
5 TRCN0000379713 TCGATCATGAATGTCCGAAGA pLKO_005 2419 3UTR 100% 4.050 3.240 N PAK4 n/a
6 TRCN0000272578 TGGACAACTTCATCAAGATTG pLKO_005 1118 CDS 100% 10.800 7.560 N PAK4 n/a
7 TRCN0000010198 CGAGAATGTGGTGGAGATGTA pLKO.1 1281 CDS 100% 4.950 3.465 N PAK4 n/a
8 TRCN0000272514 CGAGAATGTGGTGGAGATGTA pLKO_005 1281 CDS 100% 4.950 3.465 N PAK4 n/a
9 TRCN0000199898 GCAGCAAAGGTGCCAAAGATG pLKO.1 374 CDS 100% 4.950 3.465 N PAK4 n/a
10 TRCN0000010197 GAGCCACAGCGAGTATCCCAT pLKO.1 1039 CDS 100% 0.880 0.616 N PAK4 n/a
11 TRCN0000010201 CTGCTGGACGAGTTTGAGAAC pLKO.1 409 CDS 100% 4.050 2.430 N PAK4 n/a
12 TRCN0000272577 CTGCTGGACGAGTTTGAGAAC pLKO_005 409 CDS 100% 4.050 2.430 N PAK4 n/a
13 TRCN0000010199 GACTCGATCCTGCTGACCCAT pLKO.1 1486 CDS 100% 0.880 0.528 N PAK4 n/a
14 TRCN0000140719 GATCACTTGAGGTCAGGAGTT pLKO.1 5947 3UTR 100% 4.050 2.025 Y P3H4 n/a
15 TRCN0000165299 GATCACTTGAGGTCAGGAGTT pLKO.1 5947 3UTR 100% 4.050 2.025 Y ORAI2 n/a
16 TRCN0000352971 GATCACTTGAGGTCAGGAGTT pLKO_005 5947 3UTR 100% 4.050 2.025 Y P3H4 n/a
17 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 4054 3UTR 100% 5.625 2.813 Y KLHL30 n/a
18 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 4054 3UTR 100% 5.625 2.813 Y EID2B n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001014832.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02392 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_02392 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000469680 TTGCTCGCCAGCAAACCTATAACT pLX_317 24.9% 100% 100% V5 n/a
4 ccsbBroadEn_14960 pDONR223 0% 100% 100% None n/a
5 ccsbBroad304_14960 pLX_304 0% 100% 100% V5 n/a
6 TRCN0000468245 AGCAGTCATGCCACTATTTGGTAA pLX_317 18.1% 100% 100% V5 n/a
7 TRCN0000491692 AAAGCATGTTTTGACTTAAAGCCT pLX_317 13.9% 100% 100% V5 n/a
8 TRCN0000491616 GCCATTATCAGAACCCAAGTTTCC pLX_317 10.7% 100% 100% V5 (not translated due to prior stop codon) n/a
9 TRCN0000488374 CTGCGTATTCCCTACAAAGGGGTA pLX_317 17.4% 100% 100% V5 (not translated due to prior stop codon) n/a
10 TRCN0000492139 TGAGAAAAGAGGATGCAAATCATT pLX_317 22.2% 99.9% 99.8% V5 1773_1774insG n/a
11 ccsbBroadEn_11480 pDONR223 100% 72% 71.9% None 357_528del;532_854del n/a
12 ccsbBroad304_11480 pLX_304 0% 72% 71.9% V5 357_528del;532_854del n/a
13 TRCN0000480915 TTGACTCAACGCGCCATCTCGTTC pLX_317 26.7% 72% 71.9% V5 357_528del;532_854del n/a
Download CSV