Transcript: Human NM_001014835.2

Homo sapiens p21 (RAC1) activated kinase 4 (PAK4), transcript variant 4, mRNA.

Source:
NCBI, updated 2019-06-04
Taxon:
Homo sapiens (human)
Gene:
PAK4 (10298)
Length:
5783
CDS:
230..1546

Additional Resources:

NCBI RefSeq record:
NM_001014835.2
NBCI Gene record:
PAK4 (10298)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001146148 CCAGTGGCAGAGCCTGATCG pXPR_003 AGG 127 10% 3 0.3709 PAK4 PAK4 77574
2 BRDN0001148325 TGCTCATGGGATACTCGCTG pXPR_003 TGG 432 33% 4 -0.1708 PAK4 PAK4 77576
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001014835.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000272510 ACTAAGAGGTGAACATGTATG pLKO_005 1881 3UTR 100% 10.800 15.120 N PAK4 n/a
2 TRCN0000195628 CGATCATGAATGTCCGAAGAG pLKO.1 2034 3UTR 100% 4.050 5.670 N PAK4 n/a
3 TRCN0000010200 CGACCAGCACGAGCAGAAGTT pLKO.1 304 CDS 100% 1.650 2.310 N PAK4 n/a
4 TRCN0000272579 CGACCAGCACGAGCAGAAGTT pLKO_005 304 CDS 100% 1.650 2.310 N PAK4 n/a
5 TRCN0000379713 TCGATCATGAATGTCCGAAGA pLKO_005 2033 3UTR 100% 4.050 3.240 N PAK4 n/a
6 TRCN0000272578 TGGACAACTTCATCAAGATTG pLKO_005 732 CDS 100% 10.800 7.560 N PAK4 n/a
7 TRCN0000010198 CGAGAATGTGGTGGAGATGTA pLKO.1 895 CDS 100% 4.950 3.465 N PAK4 n/a
8 TRCN0000272514 CGAGAATGTGGTGGAGATGTA pLKO_005 895 CDS 100% 4.950 3.465 N PAK4 n/a
9 TRCN0000010197 GAGCCACAGCGAGTATCCCAT pLKO.1 653 CDS 100% 0.880 0.616 N PAK4 n/a
10 TRCN0000010199 GACTCGATCCTGCTGACCCAT pLKO.1 1100 CDS 100% 0.880 0.528 N PAK4 n/a
11 TRCN0000140719 GATCACTTGAGGTCAGGAGTT pLKO.1 5561 3UTR 100% 4.050 2.025 Y P3H4 n/a
12 TRCN0000165299 GATCACTTGAGGTCAGGAGTT pLKO.1 5561 3UTR 100% 4.050 2.025 Y ORAI2 n/a
13 TRCN0000352971 GATCACTTGAGGTCAGGAGTT pLKO_005 5561 3UTR 100% 4.050 2.025 Y P3H4 n/a
14 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 3668 3UTR 100% 5.625 2.813 Y KLHL30 n/a
15 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 3668 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001014835.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11480 pDONR223 100% 89.6% 84.7% None (many diffs) n/a
2 ccsbBroad304_11480 pLX_304 0% 89.6% 84.7% V5 (many diffs) n/a
3 TRCN0000480915 TTGACTCAACGCGCCATCTCGTTC pLX_317 26.7% 89.6% 84.7% V5 (many diffs) n/a
4 ccsbBroadEn_02392 pDONR223 100% 74.1% 74.1% None 202_203ins459 n/a
5 ccsbBroad304_02392 pLX_304 0% 74.1% 74.1% V5 202_203ins459 n/a
6 TRCN0000469680 TTGCTCGCCAGCAAACCTATAACT pLX_317 24.9% 74.1% 74.1% V5 202_203ins459 n/a
7 ccsbBroadEn_14960 pDONR223 0% 74.1% 74.1% None 202_203ins459 n/a
8 ccsbBroad304_14960 pLX_304 0% 74.1% 74.1% V5 202_203ins459 n/a
9 TRCN0000468245 AGCAGTCATGCCACTATTTGGTAA pLX_317 18.1% 74.1% 74.1% V5 202_203ins459 n/a
10 TRCN0000491692 AAAGCATGTTTTGACTTAAAGCCT pLX_317 13.9% 74.1% 74.1% V5 202_203ins459 n/a
11 TRCN0000491616 GCCATTATCAGAACCCAAGTTTCC pLX_317 10.7% 74.1% 74.1% V5 (not translated due to prior stop codon) 202_203ins459 n/a
12 TRCN0000488374 CTGCGTATTCCCTACAAAGGGGTA pLX_317 17.4% 74.1% 74.1% V5 (not translated due to prior stop codon) 202_203ins459 n/a
13 TRCN0000492139 TGAGAAAAGAGGATGCAAATCATT pLX_317 22.2% 74% 73.9% V5 202_203ins459;1314_1315insG n/a
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