Construct: ORF ccsbBroadEn_15007
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018892.1_s300c1, BRDN0000458188
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- MAST2 (23139)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23139 | MAST2 | microtubule associated seri... | XM_011541069.2 | 60.9% | 16.6% | (many diffs) |
| 2 | human | 23139 | MAST2 | microtubule associated seri... | XM_011541068.1 | 52% | 14.2% | (many diffs) |
| 3 | human | 23139 | MAST2 | microtubule associated seri... | XM_011541067.2 | 51.8% | 14.1% | (many diffs) |
| 4 | human | 23139 | MAST2 | microtubule associated seri... | NM_001324321.1 | 51.8% | 14.1% | (many diffs) |
| 5 | human | 23139 | MAST2 | microtubule associated seri... | XM_006710477.3 | 51.4% | 14% | (many diffs) |
| 6 | human | 23139 | MAST2 | microtubule associated seri... | XM_017000755.1 | 51.2% | 14% | (many diffs) |
| 7 | human | 23139 | MAST2 | microtubule associated seri... | XM_017000754.1 | 50.6% | 13.8% | (many diffs) |
| 8 | human | 23139 | MAST2 | microtubule associated seri... | XM_017000753.1 | 50.3% | 13.7% | (many diffs) |
| 9 | human | 23139 | MAST2 | microtubule associated seri... | XM_005270656.5 | 49.7% | 13.6% | (many diffs) |
| 10 | human | 23139 | MAST2 | microtubule associated seri... | XM_005270655.3 | 49.5% | 13.5% | (many diffs) |
| 11 | human | 23139 | MAST2 | microtubule associated seri... | XM_017000752.1 | 49.3% | 13.4% | (many diffs) |
| 12 | human | 23139 | MAST2 | microtubule associated seri... | XM_011541065.1 | 49.2% | 13.4% | (many diffs) |
| 13 | human | 23139 | MAST2 | microtubule associated seri... | NM_001319245.1 | 47.3% | 12.9% | (many diffs) |
| 14 | human | 23139 | MAST2 | microtubule associated seri... | NM_015112.3 | 47.3% | 12.9% | (many diffs) |
| 15 | human | 23139 | MAST2 | microtubule associated seri... | NM_001324320.1 | 47.1% | 12.9% | (many diffs) |
| 16 | human | 23139 | MAST2 | microtubule associated seri... | XM_011541064.2 | 46.8% | 12.8% | (many diffs) |
| 17 | human | 23139 | MAST2 | microtubule associated seri... | XM_011541063.1 | 46.7% | 12.8% | (many diffs) |
| 18 | human | 23139 | MAST2 | microtubule associated seri... | XM_011541062.2 | 45.7% | 12.5% | (many diffs) |
| 19 | human | 23139 | MAST2 | microtubule associated seri... | XM_011541061.2 | 45.5% | 12.4% | (many diffs) |
| 20 | human | 23139 | MAST2 | microtubule associated seri... | XM_011541059.2 | 45.5% | 12.4% | (many diffs) |
| 21 | mouse | 17776 | Mast2 | microtubule associated seri... | XM_006502836.3 | 45.7% | 13.3% | (many diffs) |
| 22 | mouse | 17776 | Mast2 | microtubule associated seri... | XM_006502835.3 | 45.5% | 13.3% | (many diffs) |
| 23 | mouse | 17776 | Mast2 | microtubule associated seri... | XM_006502834.2 | 44% | 12.8% | (many diffs) |
| 24 | mouse | 17776 | Mast2 | microtubule associated seri... | XM_017320018.1 | 43.4% | 12.7% | (many diffs) |
| 25 | mouse | 17776 | Mast2 | microtubule associated seri... | XM_006502840.2 | 42.1% | 12.3% | (many diffs) |
| 26 | mouse | 17776 | Mast2 | microtubule associated seri... | NM_008641.3 | 42.1% | 12.3% | (many diffs) |
| 27 | mouse | 17776 | Mast2 | microtubule associated seri... | NM_001042743.2 | 41.9% | 12.2% | (many diffs) |
| 28 | mouse | 17776 | Mast2 | microtubule associated seri... | XM_006502833.1 | 41.9% | 12.2% | (many diffs) |
| 29 | mouse | 17776 | Mast2 | microtubule associated seri... | XM_006502839.1 | 21.1% | 5.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 82
- ORF end:
- 871
- ORF length:
- 789
- Sequence:
-
1 CTGATAGTGA CCTGTTCGTT GCAACAAATT GATGAGCAAT GCTTTTTTAT AATGCCAACT 61 TTGTACAAAA AAGTTGGCAC CATGCGGCCT CGCTCCCGGA GCCTCAGTCC CGGACGATCC 121 CCAGTATCCT TTGACAGTGA AATAATAATG ATGAATCATG TTTACAAAGA AAGATTCCCA 181 AAGGCCACCG CACAAATGGA AGAGCGACTA GCAGAGTTTA TTTCCTCCAA CACTCCAGAC 241 AGCGTGCTGC CCTTGGCAGA TGGAGCCCTG AGCTTTATTC ATCATCAGGT GATTGAGATG 301 GCCCGAGACT GCCTGGATAA ATCTCGGAGT GGCCTCATTA CATCACAATA CTTCTACGAA 361 CTTCAAGATA ATTTGGAGAA ACTTTTACAA GATGCTCATG AGCGCTCAGA GAGCTCAGAA 421 GTGGCTTTTG TGATGCAGCT GGTGAAAAAG CTGATGATTA TCATTGCCCG CCCAGCACGT 481 CTCCTGGAAT GCCTGGAGTT TGACCCTGAA GAGTTCTACC ACCTTTTAGA AGCAGCTGAG 541 GGCCACGCCA AAGAGGGACA AGGGATTAAA TGTGACATTC CCCGCTACAT CGTTAGCCAG 601 CTGGGCCTCA CCCGGGATCC CCTAGAAGAA ATGGCCCAGT TGAGCAGCTG TGACAGTCCT 661 GACACTCCAG AGACAGATGA TTCTATTGAG GGCCATGGGG CATCTCTGCC ATCTAAAAAG 721 ACACCCTCTG AAGAGGACTT CGAGACCATT AAGCTCATCA GCAATGGCGC TATGGGGCTG 781 TATTTCTGGT GCGGCACAAG TCCACCCGGC AGCGCTTGCC ATGAGAGATC ACAAGCAGAC 841 TTGATCTACG GACAGATCAG CAGGCTTTCG TGAGCGTGAC ATACTGACTT CGCTGAGAAC 901 CCCTTGGTGG TCAGCAtgtt ctgctccttt gataccaagc gccacttgtg catggtgatg 961 gagtacgttg aagggggaga ctgtgccact ctgctgaaga atattggggc cctgcctgtg 1021 gacatggtgc gtctatactt tgcggaaact gtgctggccc tggagtactt acacaactat 1081 ggcatcgtgc accgtgacct caagcctgac aacctcctaa ttacatccat ggggcacatc 1141 aagctcacgg actttggact gtccaaaatg ggcctcatga gtctgacaac gaacttgtat 1201 gagggtcata ttgaaaagga tgcccgggaa ttcctggaca agcaggtatg cgggacccca 1261 gaatacattg cgcctgaggt gatcctgcgc cagggctatg ggaagccagt ggactggtgg 1321 gccatgggca ttatcctgta tgagttcctg gtgggctgcg tccctttttt tggagatact 1381 ccggaggagc tctttgggca ggtgatcagt gatgagattg tgtggcctga gggtgatgag 1441 gcactgcccc cagacgccca ggacctcacc tccaaactgc tccaccagaa ccctctggag 1501 agacttggca caggcagtgc ctatgaggtg aagcagcacc cattctttac tggtctggac 1561 tggacaggac ttctccgcca gaaggctgaa tttattcctc agttggagtc agaggatgat 1621 actagctatt ttgacacccg ctcagagcga taccaccaca tggactcgga ggatgaggaa 1681 gaagtgagtg aggatggctg ccttgagatc cgccagttct cttcctgctc tccaaggttc 1741 aacaaggtgt acagcagcat ggagcggctc tcactgctcg aggagcgccg gacaccaccc 1801 ccgaccaagc gcagcctgag tgaggagaag gaggaccatT CGGATGGCCT GGCAGGGCTC 1861 AAAGGCCGAG ACGGAGGCTG GGTGATTGGC TCCCCTGAGA TTATTACGGA AGCGGCTGTC 1921 GGTGTCTGAG TCGTCCCANC ACAGAGAGTG ACTCAAGCCC TCCAATGAAC ATGCGACGCC 1981 GCTGCTCAGG CTTCCTGGAT GCGCCTCGGT TCCCGGAGGG CCNTGAGGAG CCCAGCAGCA 2041 CCCNTCAGGA GGCAACCACA GGAGGGTATA TGGGTCCTGA CACCCCCCAT TCTGAGAGGG 2101 GGTATCTGGG CCTGTCACTG AACACTCNAG GGGAGCAGCG GCCAAAGCTG GATGAGGAAG 2161 CTGTTGGCCG GAGCAGTGGT TCCAGTCCAG CTATGGAGAC CCGAGGCCGT GGGACCTCAC 2221 AGCTGGCTGA GGGAGCCACA GCCAAGGCCA TCAGTGACCT GGCTGTGCGT AGGGCCCGCC 2281 ACCGGCTGCT CTCTGGGGAC TCAACAGAGA AGCGCACTGC TCGCCCTGTC AACAAAGTGA 2341 TCAAGTCCGC CTCAGCCACA GCCCTCTCAC TCCTCATTCC TTCGGAACAC CACACCTGCT 2401 CCCCGTTGGC CAGCCCCATG TCCCCACATT CTCAGTCGTC CAACCCATCA TCCCGGGACT 2461 CTTCTCCAAG AAGTATGGCT TCACCCTGCG GGCCATTCGC GTCTACATGG GTGACTCCGA 2521 TGTCTACACC GTGCACCATA TGGTGTGGCA CGTGGAGGAT GGAGGTCCGG CCAGTGAGGC 2581 AGGGCTTCGT CAAGGTGACC TCATCACCCA TGTCAATGGG GAACCTGTGC ATGGCCTGGT 2641 GCACACGGAG GTGGTGGAGC TTGCCAACTT TCTTGTACAA AGTTGGCATT ATAAGAAAGC 2701 ATTGCTTATC AATTTGTTGC AACGAACAGG TCACTATCAG TCAAAATAAA ATCATTATTG 2761 CCATCCCAGC CTGATAT