Transcript: Mouse XM_006502840.2

PREDICTED: Mus musculus microtubule associated serine/threonine kinase 2 (Mast2), transcript variant X7, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Mast2 (17776)
Length:
5773
CDS:
340..5721

Additional Resources:

NCBI RefSeq record:
XM_006502840.2
NBCI Gene record:
Mast2 (17776)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006502840.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000022895 CCTCATTACGTCACACTATTT pLKO.1 1470 CDS 100% 13.200 18.480 N Mast2 n/a
2 TRCN0000225740 CCTCATTACGTCACACTATTT pLKO_005 1470 CDS 100% 13.200 18.480 N Mast2 n/a
3 TRCN0000022897 CGAATGAGAAACCAATCCCTT pLKO.1 757 CDS 100% 2.640 3.696 N Mast2 n/a
4 TRCN0000219732 ACTTGTATGAGGGTCATATTG pLKO.1 2342 CDS 100% 13.200 9.240 N MAST2 n/a
5 TRCN0000225742 ACTTGTATGAGGGTCATATTG pLKO_005 2342 CDS 100% 13.200 9.240 N Mast2 n/a
6 TRCN0000333014 ACTTGTATGAGGGTCATATTG pLKO_005 2342 CDS 100% 13.200 9.240 N MAST2 n/a
7 TRCN0000225743 AGCAACAACAGGAAGGTATAT pLKO_005 3188 CDS 100% 13.200 9.240 N Mast2 n/a
8 TRCN0000218393 GTGGAAACAAGGTATCAATTT pLKO_005 3872 CDS 100% 13.200 9.240 N Mast2 n/a
9 TRCN0000225741 TTGAGACCAAGCGTCACTTAT pLKO_005 2078 CDS 100% 13.200 9.240 N Mast2 n/a
10 TRCN0000022894 CCCTGTCAACAAAGTAATCAA pLKO.1 3459 CDS 100% 5.625 3.938 N Mast2 n/a
11 TRCN0000022896 GCATCCACGAACAAGACCATA pLKO.1 5326 CDS 100% 4.950 3.465 N Mast2 n/a
12 TRCN0000022898 GCTGGTTCTGAAGAGTGGAAA pLKO.1 3858 CDS 100% 4.950 3.465 N Mast2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006502840.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15007 pDONR223 64.5% 42.1% 12.3% None (many diffs) n/a
2 ccsbBroad304_15007 pLX_304 0% 42.1% 12.3% V5 (not translated due to prior stop codon) (many diffs) n/a
3 TRCN0000467625 TGCTCCAGGTCCCAGGAGATTCCA pLX_317 12.2% 42.1% 12.3% V5 (not translated due to prior stop codon) (many diffs) n/a
4 TRCN0000488327 AACTGCACTTAACAACCAAGGTCT pLX_317 13.4% 38.9% 37.7% V5 (not translated due to prior stop codon) (many diffs) n/a
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