Transcript: Mouse NM_001042743.2

Mus musculus microtubule associated serine/threonine kinase 2 (Mast2), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-06-03
Taxon:
Mus musculus (mouse)
Gene:
Mast2 (17776)
Length:
5724
CDS:
267..5669

Additional Resources:

NCBI RefSeq record:
NM_001042743.2
NBCI Gene record:
Mast2 (17776)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001146449 AGCTGGCTAACGATGTAGCG pXPR_003 GGG 1404 26% 13 0.1826 Mast2 MAST2 76939
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001042743.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000022895 CCTCATTACGTCACACTATTT pLKO.1 1418 CDS 100% 13.200 18.480 N Mast2 n/a
2 TRCN0000225740 CCTCATTACGTCACACTATTT pLKO_005 1418 CDS 100% 13.200 18.480 N Mast2 n/a
3 TRCN0000022897 CGAATGAGAAACCAATCCCTT pLKO.1 684 CDS 100% 2.640 3.696 N Mast2 n/a
4 TRCN0000219732 ACTTGTATGAGGGTCATATTG pLKO.1 2290 CDS 100% 13.200 9.240 N MAST2 n/a
5 TRCN0000225742 ACTTGTATGAGGGTCATATTG pLKO_005 2290 CDS 100% 13.200 9.240 N Mast2 n/a
6 TRCN0000333014 ACTTGTATGAGGGTCATATTG pLKO_005 2290 CDS 100% 13.200 9.240 N MAST2 n/a
7 TRCN0000225743 AGCAACAACAGGAAGGTATAT pLKO_005 3136 CDS 100% 13.200 9.240 N Mast2 n/a
8 TRCN0000218393 GTGGAAACAAGGTATCAATTT pLKO_005 3820 CDS 100% 13.200 9.240 N Mast2 n/a
9 TRCN0000225741 TTGAGACCAAGCGTCACTTAT pLKO_005 2026 CDS 100% 13.200 9.240 N Mast2 n/a
10 TRCN0000022894 CCCTGTCAACAAAGTAATCAA pLKO.1 3407 CDS 100% 5.625 3.938 N Mast2 n/a
11 TRCN0000022896 GCATCCACGAACAAGACCATA pLKO.1 5274 CDS 100% 4.950 3.465 N Mast2 n/a
12 TRCN0000022898 GCTGGTTCTGAAGAGTGGAAA pLKO.1 3806 CDS 100% 4.950 3.465 N Mast2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001042743.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15007 pDONR223 64.5% 41.9% 12.2% None (many diffs) n/a
2 ccsbBroad304_15007 pLX_304 0% 41.9% 12.2% V5 (not translated due to prior stop codon) (many diffs) n/a
3 TRCN0000467625 TGCTCCAGGTCCCAGGAGATTCCA pLX_317 12.2% 41.9% 12.2% V5 (not translated due to prior stop codon) (many diffs) n/a
4 TRCN0000488327 AACTGCACTTAACAACCAAGGTCT pLX_317 13.4% 38.8% 37.6% V5 (not translated due to prior stop codon) (many diffs) n/a
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