Construct: ORF ccsbBroadEn_15107

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003551.2_s300c1, BRDN0000458310
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
AGK (55750)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55750 AGK acylglycerol kinase NM_018238.4 99.9% 100% 228C>T
2 human 55750 AGK acylglycerol kinase XM_011516397.3 99.9% 100% 228C>T
3 human 55750 AGK acylglycerol kinase XM_024446835.1 99.9% 100% 228C>T
4 human 55750 AGK acylglycerol kinase NM_001364948.2 82.7% 82.7% 228C>T;1045delG;1050_1051ins217
5 mouse 69923 Agk acylglycerol kinase NM_023538.2 87.5% 91.7% (many diffs)
6 mouse 69923 Agk acylglycerol kinase XM_011241458.1 83.6% 85.7% (many diffs)
7 mouse 69923 Agk acylglycerol kinase XM_006506571.2 67.9% 70.1% (many diffs)
8 mouse 69923 Agk acylglycerol kinase XM_017321733.1 43.5% 44.3% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1332
ORF length:
1266
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag
61ttggcatgac ggtgttcttt aaaacgcttc gaaatcactg gaagaaaact acagctgggc
121tctgcctgct gacctgggga ggccattggc tctatggaaa acactgtgat aacctcctaa
181ggagagcagc ctgtcaagaa gctcaggtgt ttggcaatca actcattcct cccaatgcac
241aagtgaagaa ggccactgtt tttctcaatc ctgcagcttg caaaggaaaa gctaggactc
301tatttgaaaa aaatgctgcc ccgattttac atttatctgg catggatgtg actattgtta
361agacagatta tgagggacaa gccaagaaac tcctggaact gatggaaaac acggatgtga
421tcattgttgc aggaggagat gggacactgc aggaggttgt tactggtgtt cttcgacgaa
481cagatgaggc taccttcagt aagattccca ttggatttat cccactggga gagaccagta
541gtttgagtca taccctcttt gccgaaagtg gaaacaaagt ccaacatatt actgatgcca
601cacttgccat tgtgaaagga gagacagttc cacttgatgt cttgcagatc aagggtgaaa
661aggaacagcc tgtatttgca atgaccggcc ttcgatgggg atctttcaga gatgctggcg
721tcaaagttag caagtactgg tatcttgggc ctctaaaaat caaagcagcc cactttttca
781gcactcttaa ggagtggcct cagactcatc aagcctctat ctcatacacg ggacctacag
841agagacctcc caatgaacca gaggagaccc ctgtacaaag gccttctttg tacaggagaa
901tattacgaag gcttgcgtcc tactgggcac aaccacagga tgccctttcc caagaggtga
961gcccggaggt ctggaaagat gtgcagctgt ccaccattga actgtccatc acaacacgga
1021ataatcagct tgacccgaca agcaaagaag attttctgaa tatctgcatt gaacctgaca
1081ccatcagcaa aggagacttt ataactatag gaagtcgaaa ggtgagaaac cccaagctgc
1141acgtggaggg cacggagtgt ctccaagcca gccagtgcac tttgcttatc ccggagggag
1201cagggggctc ttttagcatt gacagtgagg agtatgaagc gatgcctgtg gaggtgaaac
1261tgctccccag gaagctgcag ttcttctgtg atcctaggaa gagagaacag atgctcacaa
1321gccccaccca gtgcccaact ttcttgtaca aagttggcat tataagaaag cattgcttat
1381caatttgttg caacgaac

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