Construct: ORF ccsbBroadEn_15453

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012280.1_s300c1, BRDN0000393279
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
AGFG1 (3267)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3267 AGFG1 ArfGAP with FG repeats 1 NM_004504.4 99.8% 100% 270A>G;1416A>G
2 human 3267 AGFG1 ArfGAP with FG repeats 1 NM_001135188.1 99.5% 99.6% 270A>G;1416A>G;1536_1537insGGTGCA
3 human 3267 AGFG1 ArfGAP with FG repeats 1 XM_006712480.3 97.1% 97.2% 270A>G;1378_1425del;1464A>G
4 human 3267 AGFG1 ArfGAP with FG repeats 1 XM_005246516.4 95.7% 95.7% 270A>G;815_886del;1488A>G
5 human 3267 AGFG1 ArfGAP with FG repeats 1 NM_001135187.1 95.4% 95.3% (many diffs)
6 human 3267 AGFG1 ArfGAP with FG repeats 1 XM_006712478.4 93.2% 93.1% (many diffs)
7 human 3267 AGFG1 ArfGAP with FG repeats 1 XM_006712479.4 92.9% 92.8% (many diffs)
8 human 3267 AGFG1 ArfGAP with FG repeats 1 NM_001135189.1 92.7% 92.8% 270A>G;691_692ins120;1296A>G
9 human 3267 AGFG1 ArfGAP with FG repeats 1 XM_017003954.1 59.9% 59.4% (many diffs)
10 human 3267 AGFG1 ArfGAP with FG repeats 1 XM_006712481.3 56% 55.3% (many diffs)
11 mouse 15463 Agfg1 ArfGAP with FG repeats 1 XM_006496433.3 92.7% 96.7% (many diffs)
12 mouse 15463 Agfg1 ArfGAP with FG repeats 1 XM_006496434.3 92.5% 96.7% (many diffs)
13 mouse 15463 Agfg1 ArfGAP with FG repeats 1 NM_010472.3 92.3% 96.4% (many diffs)
14 mouse 15463 Agfg1 ArfGAP with FG repeats 1 XM_006496435.3 92.2% 96.4% (many diffs)
15 mouse 15463 Agfg1 ArfGAP with FG repeats 1 XM_006496430.3 90.1% 94.1% (many diffs)
16 mouse 15463 Agfg1 ArfGAP with FG repeats 1 XM_006496431.3 90% 94.1% (many diffs)
17 mouse 15463 Agfg1 ArfGAP with FG repeats 1 XM_006496432.3 89.7% 93.7% (many diffs)
18 mouse 15463 Agfg1 ArfGAP with FG repeats 1 NM_001310713.1 87.4% 91.6% (many diffs)
19 mouse 15463 Agfg1 ArfGAP with FG repeats 1 XM_006496438.3 87.3% 91.6% (many diffs)
20 mouse 15463 Agfg1 ArfGAP with FG repeats 1 XM_006496439.3 87.1% 91.2% (many diffs)
21 mouse 15463 Agfg1 ArfGAP with FG repeats 1 XM_006496440.3 85.7% 89.8% (many diffs)
22 mouse 15463 Agfg1 ArfGAP with FG repeats 1 NM_001347077.1 83.3% 87.3% (many diffs)
23 mouse 15463 Agfg1 ArfGAP with FG repeats 1 XM_017316914.1 82.9% 87% (many diffs)
24 mouse 15463 Agfg1 ArfGAP with FG repeats 1 XR_373537.3 56.9% (many diffs)
25 mouse 15463 Agfg1 ArfGAP with FG repeats 1 XR_001781822.1 18.5% (many diffs)
26 mouse 15463 Agfg1 ArfGAP with FG repeats 1 XR_001781825.1 18.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1752
ORF length:
1686
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag
61ttggcatggc ggccagcgcg aagcggaagc aggaggagaa gcacctgaag atgctgcggg
121acatgaccgg cctcccgcac aaccgaaagt gcttcgactg cgaccagcgc ggccccacct
181acgttaacat gacggtcggc tccttcgtgt gtacctcctg ctccggcagc ctgcgaggat
241taaatccacc acacagggtg aaatctatct ccatgacaac attcacacaa caggaaattg
301aattcttaca aaaacatgga aatgaagtct gtaagcagat ttggctagga ttatttgatg
361atagatcttc agcaattcca gacttcaggg atccacaaaa agtgaaagag tttctacaag
421aaaagtatga aaagaaaaga tggtatgtcc cgccagaaca agccaaagtc gtggcatcag
481ttcatgcatc tatttcaggg tcctctgcca gtagcacaag cagcacacct gaggtcaaac
541cactgaaatc tcttttaggg gattctgcac caacactgca cttaaataag ggcacaccta
601gtcagtcccc agttgtaggt cgttctcaag ggcagcagca ggagaagaag caatttgacc
661ttttaagtga tctcggctca gacatctttg ctgctccagc tcctcagtca acagctacag
721ccaattttgc taactttgca catttcaaca gtcatgcagc tcagaattct gcaaatgcag
781attttgcaaa ctttgatgca tttggacagt ctagtggttc gagtaatttt ggaggtttcc
841ccacagcaag tcactctcct tttcagcccc aaactacagg tggaagtgct gcatcagtaa
901atgctaattt tgctcatttt gataacttcc ccaaatcctc cagtgctgat tttggaacct
961tcaatacttc ccagagtcat caaacagcat cagctgttag taaagtttca acgaacaaag
1021ctggtttaca gactgcagac aaatatgcag cacttgctaa tttagacaat atcttcagtg
1081ccgggcaagg tggtgatcag ggaagtggct ttgggaccac aggtaaagct cctgttggtt
1141ctgtggtttc agttcccagt cagtcaagtg catcttcaga caagtatgca gctctggcag
1201aactagacag cgttttcagt tctgcagcca cctccagtaa tgcgtatact tccacaagta
1261atgctagcag caatgttttt ggaacagtgc cagtggttgc ttctgcacag acacagcctg
1321cttcatcaag tgtgcctgct ccatttggag ctacgccttc cacaaatcca tttgttgctg
1381ctgctggtcc ttctgtggca tcttctacaa acccatttca gaccaatgcc agaggagcaa
1441cagcggcaac ctttggcact gcatccatga gcatgcccac gggattcggc actcctgctc
1501cctacagtct tcccaccagc tttagtggca gctttcagca gcctgccttt ccagcccaag
1561cagctttccc tcaacagaca gctttttctc aacagcccaa tggtgcaggt tttgcagcat
1621ttggacaaac aaagccagta gtaacccctt ttggtcaagt tgcagctgct ggagtatcta
1681gtaatccttt tatgactggt gcaccaacag gacaatttcc aacaggaagc tcatcaacca
1741atcctttctt atnnccaact ttcttgtaca aagttggcat tataagaaag cattgcttat
1801caatttgttg caacgaac

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