Construct: ORF ccsbBroadEn_15907

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017469.1_s300c1, BRDN0000397965
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DDX27 (55661)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55661 DDX27 DEAD-box helicase 27 NM_017895.7 96% 95.9% 1_93del;1302C>T;2296G>A
2 human 55661 DDX27 DEAD-box helicase 27 NM_001348187.1 92.4% 92.3% (many diffs)
3 mouse 228889 Ddx27 DEAD (Asp-Glu-Ala-Asp) box ... NM_153065.3 87.5% 92.4% (many diffs)
4 mouse 228889 Ddx27 DEAD (Asp-Glu-Ala-Asp) box ... XM_006499422.1 84.7% 89.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2361
ORF length:
2295
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag
61ttggcatgct tgcggacctc ggcttaatcg gaaccatagg cgaggatgac gaggtgccgg
121tggagcccga gtctgactcc ggggacgagg aagaggaggg gcccattgtg ctgggcagac
181gacaaaaagc tttggggaag aaccgcagtg ctgatttcaa ccctgatttc gttttcactg
241agaaggaggg gacgtacgat ggcagctggg ccctggctga tgtcatgagc caactcaaga
301agaagagggc agccactaca ttagatgaga agattgagaa agttcgaaag aaaaggaaaa
361cagaggataa agaagccaag tctgggaagt tggaaaagga gaaagaagca aaggaaggct
421ctgaaccaaa ggagcaggaa gaccttcaag agaatgatga ggaaggctca gaagatgaag
481cctcggagac tgactactca tcagctgatg agaacatcct caccaaagca gatacactca
541aagtaaagga tcggaagaag aagaagaaga aaggacagga agcaggagga ttttttgaag
601atgcatctca gtacgatgaa aacctctcgt tccaggacat gaacctttcc cgccctcttc
661tgaaggccat tacagccatg ggcttcaagc agcccacccc gatccagaag gcgtgcatac
721ctgtgggtct attggggaag gacatctgtg cctgtgcagc cactgggaca ggtaaaactg
781ccgcctttgc cctgcctgtt ttggagcgtc tgatttataa accccgccag gctccagtca
841cccgcgtgct ggtgctagtg cccacccgag agctgggcat ccaggtgcac tctgtcacca
901gacagctggc ccagttctgc aacatcacca cctgcctggc tgtgggcggc ttggatgtga
961agtctcagga agcagctctt cgggcagcgc ctgacatcct catcgccacc ccaggccggc
1021tcatcgatca cctccacaac tgcccttcct tccacctgag cagcatcgag gtgctcatcc
1081tggacgaggc tgacaggatg ctggatgagt actttgagga gcagatgaag gagatcatcc
1141gaatgtgttc ccaccaccgc cagaccatgc tcttctcggc caccatgaca gacgaggtga
1201aagatctggc ttctgtctcc ttgaagaatc ctgtccggat atttgtgaac agcaacacag
1261atgtggctcc ctttctgcgg caggagttca tccggatccg gcctaatcgt gaaggagacc
1321gggaagccat cgtggcagct ttgttgacga ggaccttcac tgaccatgtg atgctgttca
1381cgcaaaccaa gaagcaggcc caccgcatgc acatcctcct ggggctcatg gggctgcagg
1441tgggtgagct ccatggcaac ttgtcacaga cgcagcggct ggaggccctc cggcgtttta
1501aggatgaaca gattgacatc ctcgtggcca ctgatgtggc agcccgtgga cttgacattg
1561agggggtcaa aacggtaatc aacttcacaa tgcctaatac catcaaacat tatgtccacc
1621gggtggggcg aacagcacgt gctggcaggg ctgggcgctc agtctctctg gtgggagaag
1681atgagcggaa gatgctgaag gagattgtaa aagctgccaa ggcccctgtg aaggccagga
1741tacttcccca agatgtcatc ctcaaattcc gggacaagat tgagaaaatg gagaaagatg
1801tgtatgcagt tctgcagcta gaggcggagg aaaaagagat gcagcagtca gaagcccaga
1861tcaatacagc aaagcggctc ctggagaagg ggaaggaggc agtggtccaa gagcccgaga
1921ggagctggtt ccagaccaaa gaagagagga agaaggagaa aattgccaaa gctctgcagg
1981aatttgactt ggccttaaga ggaaagaaga aaaggaagaa gtttatgaag gatgccaaaa
2041aaaaggggga gatgacagca gaggaaaggt ctcagtttga aatcctcaag gcgcagatgt
2101ttgctgaacg gctagcgaag aggaatcgca gagccaagcg ggcccgagca atgcccgagg
2161aggagccagt gagaggtcct gccaagaagc aaaagcaggg gaagaaatct gtatttgatg
2221aagaactcac caacacaagc aagaaggccc tgaaacagta tcgagctagc ccttcctttg
2281aagaaaggaa acagttgggc ttgccccacc agagacgagg aggaaacttt aaatctaaat
2341ccagatacaa gaggaggaag tnnccaactt tcttgtacaa agttggcatt ataagaaagc
2401attgcttatc aatttgttgc aacgaac

Download FASTA (ORF) (Full)

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