Construct: ORF ccsbBroadEn_15938
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000817.1_s300c1, BRDN0000397870
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RTN4 (57142)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57142 | RTN4 | reticulon 4 | NM_153828.3 | 91.7% | 91.6% | (many diffs) |
| 2 | human | 57142 | RTN4 | reticulon 4 | NM_207520.2 | 87.3% | 87.2% | (many diffs) |
| 3 | human | 57142 | RTN4 | reticulon 4 | NM_007008.3 | 54.7% | 54.8% | (many diffs) |
| 4 | human | 57142 | RTN4 | reticulon 4 | NR_135829.1 | 31.4% | (many diffs) | |
| 5 | human | 57142 | RTN4 | reticulon 4 | NR_135830.2 | 28.9% | (many diffs) | |
| 6 | human | 57142 | RTN4 | reticulon 4 | NM_020532.5 | 28.7% | 28.6% | (many diffs) |
| 7 | human | 57142 | RTN4 | reticulon 4 | NM_001321859.2 | 26.5% | 22.8% | (many diffs) |
| 8 | human | 57142 | RTN4 | reticulon 4 | NM_001321860.1 | 26.5% | 22.8% | (many diffs) |
| 9 | human | 57142 | RTN4 | reticulon 4 | NM_001321861.1 | 26.5% | 22.8% | (many diffs) |
| 10 | human | 57142 | RTN4 | reticulon 4 | NM_001321862.1 | 26.5% | 22.8% | (many diffs) |
| 11 | human | 57142 | RTN4 | reticulon 4 | NM_001321863.2 | 26.5% | 22.8% | (many diffs) |
| 12 | human | 57142 | RTN4 | reticulon 4 | NM_001321904.2 | 26.5% | 22.8% | (many diffs) |
| 13 | human | 57142 | RTN4 | reticulon 4 | NM_207521.2 | 26.5% | 22.8% | (many diffs) |
| 14 | mouse | 68585 | Rtn4 | reticulon 4 | NM_194052.3 | 77.7% | 80.2% | (many diffs) |
| 15 | mouse | 68585 | Rtn4 | reticulon 4 | NM_194053.3 | 73.9% | 76% | (many diffs) |
| 16 | mouse | 68585 | Rtn4 | reticulon 4 | NM_024226.4 | 50.9% | 54.5% | (many diffs) |
| 17 | mouse | 68585 | Rtn4 | reticulon 4 | NM_194054.3 | 25.9% | 25.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1095
- ORF length:
- 1029
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag 61 ttggcatgga agacgaggag gaagaagagg aggaggaaga ggaggacgag gacgaagacc 121 tggaggagct ggaggtgctg gagaggaagc ccgccgccgg gctgtccgcg gccccagtgc 181 ccaccgcccc tgccgccggc gcgcccctga tggacttcgg aaatgacttc gtgccgccgg 241 cgccccgggg acccctgccg gccgctcccc ccgtcgcccc ggagcggcag ccgtcttggg 301 acccgagccc ggtgtcgtcg accgtgcccg cgccatcccc gctgtctgct gccgcagtct 361 cgccctccaa gctccctgag gacgacgagc ctccggcccg gcctccccct cctcccccgg 421 ccagcgtgag cccccaggca gagcccgtgt ggaccccgcc agccccggct cccgccgcgc 481 ccccctccac cccggccgcg cccaagcgca ggggctcctc gggctcagtg gttgttgacc 541 tcctgtactg gagagacatt aagaagactg gagtggtgtt tggtgccagc ctattcctgc 601 tgctttcatt gacagtattc agcattgtga gcgtaacagc ctacattgcc ttggccctgc 661 tctctgtgac catcagcttt aggatataca agggtgtgat ccaagctatc cagaaatcag 721 atgaaggcca cccattcagg gcatatctgg aatctgaagt tgctatatct gaggagttgg 781 ttcagaagta cagtaattct gctcttggtc atgtgaactg cacgataaag gaactcaggc 841 gcctcttctt agttgatgat ttagttgatt ctctgaagtt tgcagtgttg atgtgggtat 901 ttacctatgt tggtgccttg tttaatggtc tgacactact gattttggct ctcatttcac 961 tcttcagtgt tcctgttatt tatgaacggc atcaggcaca gatagatcat tatctaggac 1021 ttgcaaataa gaatgttaaa gatgctatgg ctaaaatcca agcaaaaatc cctggattga 1081 agcgcaaagc tgaatnncca actttcttgt acaaagttgg cattataaga aagcattgct 1141 tatcaatttg ttgcaacgaa c