Transcript: Human NM_207521.2

Homo sapiens reticulon 4 (RTN4), transcript variant 5, mRNA.

Source:
NCBI, updated 2019-08-27
Taxon:
Homo sapiens (human)
Gene:
RTN4 (57142)
Length:
4068
CDS:
141..3101

Additional Resources:

NCBI RefSeq record:
NM_207521.2
NBCI Gene record:
RTN4 (57142)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_207521.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000417227 GTATGGATTTAAACCGTAATC pLKO_005 3313 3UTR 100% 10.800 15.120 N RTN4 n/a
2 TRCN0000420965 AGACGAGATCATACCGGTAAA pLKO_005 3535 3UTR 100% 10.800 8.640 N RTN4 n/a
3 TRCN0000071688 GCAGTGTTGATGTGGGTATTT pLKO.1 2886 CDS 100% 13.200 9.240 N Rtn4 n/a
4 TRCN0000179649 GCAGTGTTGATGTGGGTATTT pLKO.1 2886 CDS 100% 13.200 9.240 N RTN4 n/a
5 TRCN0000183585 GCTGCTTTCATTGACAGTATT pLKO.1 2603 CDS 100% 13.200 9.240 N RTN4 n/a
6 TRCN0000147624 GCATATCTGGAATCTGAAGTT pLKO.1 2745 CDS 100% 4.950 3.465 N RTN4 n/a
7 TRCN0000179762 GCTATATCTGAGGAGTTGGTT pLKO.1 2766 CDS 100% 3.000 2.100 N RTN4 n/a
8 TRCN0000147846 GAAGTACAGTAATTCTGCTCT pLKO.1 2789 CDS 100% 2.640 1.848 N RTN4 n/a
9 TRCN0000146691 CCTGTTATTTATGAACGGCAT pLKO.1 2976 CDS 100% 2.160 1.512 N RTN4 n/a
10 TRCN0000435974 AGTTGATGATTTAGTTGATTC pLKO_005 2855 CDS 100% 10.800 6.480 N RTN4 n/a
11 TRCN0000375427 CCACCCATTCAGGGCATATTT pLKO_005 2732 CDS 100% 15.000 21.000 N Rtn4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_207521.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03793 pDONR223 100% 31.3% 23.3% None (many diffs) n/a
2 ccsbBroad304_03793 pLX_304 0% 31.3% 23.3% V5 (many diffs) n/a
3 TRCN0000473077 TTAAATCCGCAGGACACTGCGCTG pLX_317 37.4% 31.3% 23.3% V5 (many diffs) n/a
4 ccsbBroadEn_03794 pDONR223 100% 30.8% 23.4% None (many diffs) n/a
5 ccsbBroad304_03794 pLX_304 0% 30.8% 23.4% V5 (many diffs) n/a
6 TRCN0000468620 AGCCAACAGCAGTTTGATTGCGGT pLX_317 33.1% 30.8% 23.4% V5 (many diffs) n/a
7 ccsbBroadEn_15938 pDONR223 0% 26.5% 22.8% None (many diffs) n/a
8 ccsbBroadEn_15937 pDONR223 0% 19.7% 19.3% None (many diffs) n/a
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