Construct: ORF ccsbBroadEn_15952

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004751.1_s300c1, BRDN0000395497
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MIER1 (57708)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 57708 MIER1 MIER1 transcriptional regul... NM_001077704.3 36% 36% 1_129del;598_1299del
2 human 57708 MIER1 MIER1 transcriptional regul... NM_001146113.1 34.8% 34.8% 0_1ins60;409_1110del
3 human 57708 MIER1 MIER1 transcriptional regul... NM_001077702.3 34.6% 34.6% 1_180del;649_1350del
4 human 57708 MIER1 MIER1 transcriptional regul... NM_001146111.1 33.1% 33.1% 1_180del;649_1410del
5 human 57708 MIER1 MIER1 transcriptional regul... NM_001146112.2 32.1% 32.1% 1_48del;517_1455del
6 human 57708 MIER1 MIER1 transcriptional regul... XM_017001931.1 32.1% 32.1% 1_48del;517_1455del
7 human 57708 MIER1 MIER1 transcriptional regul... NM_001077703.3 32% 32% 1_288del;757_1458del
8 human 57708 MIER1 MIER1 transcriptional regul... XM_017001927.1 30.5% 30.5% 1_126del;595_1533del
9 human 57708 MIER1 MIER1 transcriptional regul... XM_017001928.2 30.5% 30.5% 1_126del;595_1533del
10 human 57708 MIER1 MIER1 transcriptional regul... XM_017001929.1 30.5% 30.5% 1_126del;595_1533del
11 human 57708 MIER1 MIER1 transcriptional regul... XM_017001930.1 30.5% 30.5% 1_126del;595_1533del
12 human 57708 MIER1 MIER1 transcriptional regul... NM_001077701.3 30.4% 30.4% 1_129del;598_1536del
13 human 57708 MIER1 MIER1 transcriptional regul... NM_001146110.2 29.4% 29.4% 1_180del;649_1587del
14 human 57708 MIER1 MIER1 transcriptional regul... NM_020948.4 29.4% 29.4% 1_180del;649_1587del
15 human 57708 MIER1 MIER1 transcriptional regul... XM_005271076.3 29.4% 29.4% 1_180del;649_1587del
16 human 57708 MIER1 MIER1 transcriptional regul... XM_017001924.1 29.4% 29.4% 1_180del;649_1587del
17 human 57708 MIER1 MIER1 transcriptional regul... XM_017001925.1 29.4% 29.4% 1_180del;649_1587del
18 human 57708 MIER1 MIER1 transcriptional regul... XM_017001926.1 29.4% 29.4% 1_180del;649_1587del
19 human 57708 MIER1 MIER1 transcriptional regul... XM_017001932.1 28.9% 28.9% 0_1ins60;409_1347del
20 human 57708 MIER1 MIER1 transcriptional regul... XM_017001933.1 28.9% 28.9% 0_1ins60;409_1347del
21 human 57708 MIER1 MIER1 transcriptional regul... XM_017001934.1 28.9% 28.9% 0_1ins60;409_1347del
22 human 57708 MIER1 MIER1 transcriptional regul... XM_017001921.1 28.6% 28.6% 1_228del;697_1635del
23 human 57708 MIER1 MIER1 transcriptional regul... XM_017001922.1 28.6% 28.6% 1_228del;697_1635del
24 human 57708 MIER1 MIER1 transcriptional regul... XM_017001919.1 28.4% 28.4% 1_237del;706_1644del
25 human 57708 MIER1 MIER1 transcriptional regul... XM_017001920.1 28.4% 28.4% 1_237del;706_1644del
26 human 57708 MIER1 MIER1 transcriptional regul... XM_017001923.1 28.1% 26.4% (many diffs)
27 human 57708 MIER1 MIER1 transcriptional regul... NM_001077700.3 27.6% 27.6% 1_288del;757_1695del
28 human 57708 MIER1 MIER1 transcriptional regul... XM_011541865.2 26.4% 26.4% 1_360del;829_1767del
29 human 57708 MIER1 MIER1 transcriptional regul... NM_001350530.2 25.9% 25.9% 1_399del;868_1806del
30 human 57708 MIER1 MIER1 transcriptional regul... XR_001737345.1 9.5% 1_442del;911_4888del
31 mouse 71148 Mier1 MEIR1 treanscription regulator XM_017320386.1 29.4% 31.1% (many diffs)
32 mouse 71148 Mier1 MEIR1 treanscription regulator NM_001286223.1 27.9% 29.5% (many diffs)
33 mouse 71148 Mier1 MEIR1 treanscription regulator NM_001039081.2 27.9% 29.4% (many diffs)
34 mouse 71148 Mier1 MEIR1 treanscription regulator NM_001286222.1 27% 28.5% (many diffs)
35 mouse 71148 Mier1 MEIR1 treanscription regulator NM_027696.3 27% 28.5% (many diffs)
36 mouse 71148 Mier1 MEIR1 treanscription regulator XM_006503401.3 27% 28.5% (many diffs)
37 mouse 71148 Mier1 MEIR1 treanscription regulator XM_006503402.1 27% 28.5% (many diffs)
38 mouse 71148 Mier1 MEIR1 treanscription regulator NM_001286221.1 25.6% 27.1% (many diffs)
39 mouse 71148 Mier1 MEIR1 treanscription regulator XM_006503399.2 25.3% 26.8% (many diffs)
40 mouse 71148 Mier1 MEIR1 treanscription regulator XM_006503398.2 24.7% 26.1% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
534
ORF length:
468
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag
61ttggcatgat ggaaggagaa acaaacttca gctctgaaat agaagatctt gcaagggaag
121gcgacatgcc aattcatgaa cttctcagcc tttatggtta tggtagtact gttcgactac
181ctgaagaaga tgaggaagag gaagaagagg aagaagaagg tgaagatgat gaagatgctg
241ataatgatga caacagtggc tgtagtgggg aaaataaaga ggagaatata aaggattcat
301caggtcagga ggatgaaact cagtcttcca atgatgatcc atcacaatct gttgcttctc
361aagatgccca ggaaataatc cgcccacgtc gatgtaaata ttttgataca aatagtgaag
421tagaagaaga atctgaagaa gatgaagatt atattccatc agaagactgg aaaaaggaga
481ttatggtggg ctccatgttt caagcagaaa ttccagttgg catttgtaga tactnnccaa
541ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac

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