Transcript: Human NM_001146111.1

Homo sapiens MIER1 transcriptional regulator (MIER1), transcript variant 7, mRNA.

Source:
NCBI, updated 2019-08-04
Taxon:
Homo sapiens (human)
Gene:
MIER1 (57708)
Length:
3893
CDS:
317..1729

Additional Resources:

NCBI RefSeq record:
NM_001146111.1
NBCI Gene record:
MIER1 (57708)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001146111.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000331339 ATCCGCCCACGTCGATGTAAA pLKO_005 809 CDS 100% 13.200 18.480 N MIER1 n/a
2 TRCN0000108034 CAGAAATTCCAGTTGGCATTT pLKO.1 936 CDS 100% 10.800 8.640 N MIER1 n/a
3 TRCN0000300137 CAGAAATTCCAGTTGGCATTT pLKO_005 936 CDS 100% 10.800 8.640 N MIER1 n/a
4 TRCN0000108033 GCCTTTATGGTTATGGTAGTA pLKO.1 579 CDS 100% 4.950 3.960 N MIER1 n/a
5 TRCN0000095737 CCCACGTCGATGTAAATATTT pLKO.1 814 CDS 100% 15.000 10.500 N Mier1 n/a
6 TRCN0000108030 CCCATGAAGTATTACTGTTAA pLKO.1 1818 3UTR 100% 13.200 9.240 N MIER1 n/a
7 TRCN0000303943 TTTGATGATGAACGAACATTA pLKO_005 464 CDS 100% 13.200 9.240 N MIER1 n/a
8 TRCN0000108031 CCAATGATGATCCATCACAAT pLKO.1 759 CDS 100% 4.950 3.465 N MIER1 n/a
9 TRCN0000095734 GCATTCTATTACATGTGGAAA pLKO.1 1340 CDS 100% 4.950 3.465 N Mier1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001146111.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03845 pDONR223 100% 78.7% 78.7% None 1_240del;1280_1339del n/a
2 ccsbBroad304_03845 pLX_304 0% 78.7% 78.7% V5 1_240del;1280_1339del n/a
3 TRCN0000476937 CTTTTGCCGTTTTACCGCAAAGCA pLX_317 40.1% 78.7% 78.7% V5 1_240del;1280_1339del n/a
4 ccsbBroadEn_15952 pDONR223 0% 33.1% 33.1% None 1_180del;649_1410del n/a
5 ccsbBroad304_15952 pLX_304 0% 33.1% 33.1% V5 1_180del;649_1410del n/a
6 TRCN0000480736 TATCGCCCGGGTTTCGGTTGCTCG pLX_317 94.8% 33.1% 33.1% V5 1_180del;649_1410del n/a
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