Transcript: Human NM_001009183.1

Homo sapiens gem nuclear organelle associated protein 2 (GEMIN2), transcript variant gamma, mRNA.

Source:
NCBI, updated 2019-08-04
Taxon:
Homo sapiens (human)
Gene:
GEMIN2 (8487)
Length:
1309
CDS:
23..775

Additional Resources:

NCBI RefSeq record:
NM_001009183.1
NBCI Gene record:
GEMIN2 (8487)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001009183.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000033435 GCTTAGTATTGTTAGCAGAAT pLKO.1 559 CDS 100% 4.950 6.930 N GEMIN2 n/a
2 TRCN0000033438 CCTCCCTTGCTTAGTATTGTT pLKO.1 551 CDS 100% 5.625 3.938 N GEMIN2 n/a
3 TRCN0000299583 CCTCCCTTGCTTAGTATTGTT pLKO_005 551 CDS 100% 5.625 3.938 N GEMIN2 n/a
4 TRCN0000033434 CGACAGAATGTGAACAAACAT pLKO.1 362 CDS 100% 5.625 3.938 N GEMIN2 n/a
5 TRCN0000299528 CGACAGAATGTGAACAAACAT pLKO_005 362 CDS 100% 5.625 3.938 N GEMIN2 n/a
6 TRCN0000033436 CCAGATGTTGTGGTAGCTCAA pLKO.1 212 CDS 100% 4.050 2.835 N GEMIN2 n/a
7 TRCN0000310428 CCAGATGTTGTGGTAGCTCAA pLKO_005 212 CDS 100% 4.050 2.835 N GEMIN2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001009183.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01938 pDONR223 100% 89.2% 88.5% None 745_746insA;747_748ins25;750_751ins64 n/a
2 ccsbBroad304_01938 pLX_304 37.3% 89.2% 88.5% V5 745_746insA;747_748ins25;750_751ins64 n/a
3 TRCN0000476259 TGCTGTAGAAACTTGGTTCCACCG pLX_317 37.5% 89.2% 88.5% V5 745_746insA;747_748ins25;750_751ins64 n/a
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