Transcript: Mouse NM_001033531.4

Mus musculus kelch-like 32 (Klhl32), transcript variant 2, mRNA.

Source:
NCBI, updated 2018-05-26
Taxon:
Mus musculus (mouse)
Gene:
Klhl32 (212390)
Length:
7053
CDS:
450..2213

Additional Resources:

NCBI RefSeq record:
NM_001033531.4
NBCI Gene record:
Klhl32 (212390)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001033531.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000252681 GTCCGATACTCTCTATATTAT pLKO_005 1211 CDS 100% 15.000 21.000 N Klhl32 n/a
2 TRCN0000252682 GATCAGTGGACGCGCTGTAAT pLKO_005 1920 CDS 100% 13.200 18.480 N Klhl32 n/a
3 TRCN0000252683 TCTCGTGTCACGCTGGATATG pLKO_005 1657 CDS 100% 10.800 15.120 N Klhl32 n/a
4 TRCN0000267458 CCGGATTCTCGTGCGTCATAT pLKO_005 1877 CDS 100% 13.200 10.560 N Klhl32 n/a
5 TRCN0000122442 GCTGTCCATAATGGGAGAATA pLKO.1 1977 CDS 100% 13.200 9.240 N KLHL32 n/a
6 TRCN0000267431 TACATCCGCTTTGGCCTAATG pLKO_005 1038 CDS 100% 10.800 7.560 N Klhl32 n/a
7 TRCN0000106535 GCCTGGTCTACAAAGTGAGTT pLKO.1 6380 3UTR 100% 4.950 2.475 Y Gad2 n/a
8 TRCN0000166364 CACACACACACACACACACAA pLKO.1 5836 3UTR 100% 4.950 2.475 Y KAAG1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001033531.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13037 pDONR223 100% 63.3% 65.9% None (many diffs) n/a
2 ccsbBroad304_13037 pLX_304 0% 63.3% 65.9% V5 (many diffs) n/a
3 TRCN0000471330 GTTTAGTAACCGAACCCGCTACAT pLX_317 28.1% 63.3% 65.9% V5 (many diffs) n/a
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