Construct: ORF TRCN0000471330
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016875.1_s317c1
- Derived from:
- ccsbBroadEn_13037
- DNA Barcode:
- GTTTAGTAACCGAACCCGCTACAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KLHL32 (114792)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471330
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 114792 | KLHL32 | kelch like family member 32 | NM_001286252.2 | 100% | 100% | |
| 2 | human | 114792 | KLHL32 | kelch like family member 32 | NM_001323253.1 | 89.1% | 87.4% | (many diffs) |
| 3 | human | 114792 | KLHL32 | kelch like family member 32 | NM_001323255.2 | 84.6% | 84.6% | 408_409ins216 |
| 4 | human | 114792 | KLHL32 | kelch like family member 32 | NM_001323254.1 | 75.3% | 73.6% | (many diffs) |
| 5 | human | 114792 | KLHL32 | kelch like family member 32 | NM_001323252.1 | 73.9% | 72.9% | (many diffs) |
| 6 | human | 114792 | KLHL32 | kelch like family member 32 | NM_052904.4 | 73.9% | 72.9% | (many diffs) |
| 7 | human | 114792 | KLHL32 | kelch like family member 32 | NM_001286250.1 | 68.1% | 67.2% | (many diffs) |
| 8 | human | 114792 | KLHL32 | kelch like family member 32 | NM_001323263.1 | 63.3% | 62% | (many diffs) |
| 9 | human | 114792 | KLHL32 | kelch like family member 32 | NM_001286251.1 | 62.8% | 61.8% | (many diffs) |
| 10 | human | 114792 | KLHL32 | kelch like family member 32 | XM_005266813.5 | 62.8% | 61.8% | (many diffs) |
| 11 | human | 114792 | KLHL32 | kelch like family member 32 | NM_001323257.1 | 62.4% | 61.4% | (many diffs) |
| 12 | human | 114792 | KLHL32 | kelch like family member 32 | NM_001323264.1 | 51.8% | 50.4% | (many diffs) |
| 13 | human | 114792 | KLHL32 | kelch like family member 32 | XM_017010228.2 | 51.3% | 50.3% | (many diffs) |
| 14 | human | 114792 | KLHL32 | kelch like family member 32 | NM_001323256.2 | 51.1% | 45.7% | 627_705del;840_841ins649 |
| 15 | human | 114792 | KLHL32 | kelch like family member 32 | NM_001323262.1 | 46.5% | 44.8% | (many diffs) |
| 16 | human | 114792 | KLHL32 | kelch like family member 32 | NM_001323258.1 | 44.6% | 44.6% | 630_630delGins781 |
| 17 | human | 114792 | KLHL32 | kelch like family member 32 | NM_001323259.2 | 44.6% | 44.6% | 630_630delGins781 |
| 18 | human | 114792 | KLHL32 | kelch like family member 32 | XM_017010229.2 | 41% | 39.7% | (many diffs) |
| 19 | human | 114792 | KLHL32 | kelch like family member 32 | NM_001323260.1 | 38.2% | 37.1% | (many diffs) |
| 20 | human | 114792 | KLHL32 | kelch like family member 32 | NM_001323261.1 | 38.2% | 37.1% | (many diffs) |
| 21 | human | 114792 | KLHL32 | kelch like family member 32 | XR_001743153.2 | 15.8% | (many diffs) | |
| 22 | mouse | 212390 | Klhl32 | kelch-like 32 | NM_001163020.2 | 68.2% | 71.2% | (many diffs) |
| 23 | mouse | 212390 | Klhl32 | kelch-like 32 | NM_001033531.4 | 63.3% | 65.9% | (many diffs) |
| 24 | mouse | 212390 | Klhl32 | kelch-like 32 | XM_017320094.2 | 46.5% | 44.1% | (many diffs) |
| 25 | mouse | 212390 | Klhl32 | kelch-like 32 | NM_001355618.1 | 33.8% | 34.8% | (many diffs) |
| 26 | mouse | 212390 | Klhl32 | kelch-like 32 | XM_030253379.1 | 33.8% | 34.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1476
- ORF length:
- 1410
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc gtctgaacgc tgcctcagta ttcaagaaat gctgacaggc cagaggctct 121 gccactccga atctcacaat gacagtgtcc tggcagcgct gaatcagcag aggagtgatg 181 gcatcctctg cgacatcacc ctgattgctg aggaacagaa attccatgct cacaaggcag 241 tcctagcagc atgcagtgac tatttccggg caatgttcag tctttgtatg gtggaaagtg 301 gagctgatga ggttaatttg cacggtgtga ccagccttgg cttaaagcag gctctggagt 361 ttgcatacac aggacagatt ttgctggagc caggtgtgat ccaggatgtg ctagcagcgg 421 gcagtcacct acagctgttg gagcttctca atttatgctc ccactatctc atccaggaat 481 taaatagctt taattacttg gatctgtaca gacttgctga cctctttaac ctcactttgt 541 tggagaaggc agtgatcgat ttcttagtga aacatctctc tgaactcctg aagagccgcc 601 cagaagaagt tctaacgctt ccctattgcc tgcttcagga ggtgctgaag agcgaccgcc 661 tgacctccct gagtgaagag cagatctggc agctagctgt gaggtggttg gaacacaact 721 gccactacca gtacatggac gagctcctgc aatacatccg ctttggccta atggatgtgg 781 atactctcca tacagttgcc ctgtcccacc cccttgtcca agcaagtgag actgcaacag 841 cccttgtcaa cgaggccctg gaataccacc agagcatcta tgcacagcct gtctggcaga 901 ctcgcaggac caaaccacga ttccagtcag acactctgta tatcattggt gggaaaaagc 961 gcgaggtctg caaggtcaag gaacttcggt acttcaatcc tgttgatcag gagaatgctc 1021 tcatagctgc cattgccaac tggagtgagc tggctcccat gcctgtggga aggagccacc 1081 attGTGTGGC AGTCATGGGG GACTTCCTGT TTGTGGCAGG AGGGGAAGTT GAGCATGCCA 1141 GTGGCCGGAC GTGTGCTGTG AGGACTGCCT GTCGCTATGA CCCCCGCAGT AATTCCTGGG 1201 CAGAGATAGC ACCCATGAAA AACTGCCGGG AGCATTTTGT GCTGGGTGCC ATGGAGGAAT 1261 ACCTCTATGC AGTTGGGGGC AGAAATGAAC TGCGCCAGGT TCTGCCTACA GTTGAGCGAT 1321 ATTGCCCCAA GAAGAACAAA TGGACTTTTG TTCAGTCCTT TGACAGATCC CTTTCATGCC 1381 ATGCTGGATA TGTGGCTGAT GGTCTTCTTT GGATATCAGG TAGAACATAC CTCATGTTGG 1441 ATTTATCAAA ACACACTTTC ATTGTGGTAT ATATTTACCC AACTTTCTTG TACAAAGTGG 1501 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1561 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1621 AGTTTAGTAA CCGAACCCGC TACATACGCG TTAAGTCgac aatcaacctc tggattacaa 1681 aatttgtgaa agatt