Construct: ORF TRCN0000471330

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016875.1_s317c1
Derived from:
ccsbBroadEn_13037
DNA Barcode:
GTTTAGTAACCGAACCCGCTACAT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
KLHL32 (114792)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000471330
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 114792 KLHL32 kelch like family member 32 NM_001286252.2 100% 100%
2 human 114792 KLHL32 kelch like family member 32 NM_001323253.1 89.1% 87.4% (many diffs)
3 human 114792 KLHL32 kelch like family member 32 NM_001323255.2 84.6% 84.6% 408_409ins216
4 human 114792 KLHL32 kelch like family member 32 NM_001323254.1 75.3% 73.6% (many diffs)
5 human 114792 KLHL32 kelch like family member 32 NM_001323252.1 73.9% 72.9% (many diffs)
6 human 114792 KLHL32 kelch like family member 32 NM_052904.4 73.9% 72.9% (many diffs)
7 human 114792 KLHL32 kelch like family member 32 NM_001286250.1 68.1% 67.2% (many diffs)
8 human 114792 KLHL32 kelch like family member 32 NM_001323263.1 63.3% 62% (many diffs)
9 human 114792 KLHL32 kelch like family member 32 NM_001286251.1 62.8% 61.8% (many diffs)
10 human 114792 KLHL32 kelch like family member 32 XM_005266813.5 62.8% 61.8% (many diffs)
11 human 114792 KLHL32 kelch like family member 32 NM_001323257.1 62.4% 61.4% (many diffs)
12 human 114792 KLHL32 kelch like family member 32 NM_001323264.1 51.8% 50.4% (many diffs)
13 human 114792 KLHL32 kelch like family member 32 XM_017010228.2 51.3% 50.3% (many diffs)
14 human 114792 KLHL32 kelch like family member 32 NM_001323256.2 51.1% 45.7% 627_705del;840_841ins649
15 human 114792 KLHL32 kelch like family member 32 NM_001323262.1 46.5% 44.8% (many diffs)
16 human 114792 KLHL32 kelch like family member 32 NM_001323258.1 44.6% 44.6% 630_630delGins781
17 human 114792 KLHL32 kelch like family member 32 NM_001323259.2 44.6% 44.6% 630_630delGins781
18 human 114792 KLHL32 kelch like family member 32 XM_017010229.2 41% 39.7% (many diffs)
19 human 114792 KLHL32 kelch like family member 32 NM_001323260.1 38.2% 37.1% (many diffs)
20 human 114792 KLHL32 kelch like family member 32 NM_001323261.1 38.2% 37.1% (many diffs)
21 human 114792 KLHL32 kelch like family member 32 XR_001743153.2 15.8% (many diffs)
22 mouse 212390 Klhl32 kelch-like 32 NM_001163020.2 68.2% 71.2% (many diffs)
23 mouse 212390 Klhl32 kelch-like 32 NM_001033531.4 63.3% 65.9% (many diffs)
24 mouse 212390 Klhl32 kelch-like 32 XM_017320094.2 46.5% 44.1% (many diffs)
25 mouse 212390 Klhl32 kelch-like 32 NM_001355618.1 33.8% 34.8% (many diffs)
26 mouse 212390 Klhl32 kelch-like 32 XM_030253379.1 33.8% 34.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1476
ORF length:
1410
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgcc gtctgaacgc tgcctcagta ttcaagaaat gctgacaggc cagaggctct
121gccactccga atctcacaat gacagtgtcc tggcagcgct gaatcagcag aggagtgatg
181gcatcctctg cgacatcacc ctgattgctg aggaacagaa attccatgct cacaaggcag
241tcctagcagc atgcagtgac tatttccggg caatgttcag tctttgtatg gtggaaagtg
301gagctgatga ggttaatttg cacggtgtga ccagccttgg cttaaagcag gctctggagt
361ttgcatacac aggacagatt ttgctggagc caggtgtgat ccaggatgtg ctagcagcgg
421gcagtcacct acagctgttg gagcttctca atttatgctc ccactatctc atccaggaat
481taaatagctt taattacttg gatctgtaca gacttgctga cctctttaac ctcactttgt
541tggagaaggc agtgatcgat ttcttagtga aacatctctc tgaactcctg aagagccgcc
601cagaagaagt tctaacgctt ccctattgcc tgcttcagga ggtgctgaag agcgaccgcc
661tgacctccct gagtgaagag cagatctggc agctagctgt gaggtggttg gaacacaact
721gccactacca gtacatggac gagctcctgc aatacatccg ctttggccta atggatgtgg
781atactctcca tacagttgcc ctgtcccacc cccttgtcca agcaagtgag actgcaacag
841cccttgtcaa cgaggccctg gaataccacc agagcatcta tgcacagcct gtctggcaga
901ctcgcaggac caaaccacga ttccagtcag acactctgta tatcattggt gggaaaaagc
961gcgaggtctg caaggtcaag gaacttcggt acttcaatcc tgttgatcag gagaatgctc
1021tcatagctgc cattgccaac tggagtgagc tggctcccat gcctgtggga aggagccacc
1081attGTGTGGC AGTCATGGGG GACTTCCTGT TTGTGGCAGG AGGGGAAGTT GAGCATGCCA
1141GTGGCCGGAC GTGTGCTGTG AGGACTGCCT GTCGCTATGA CCCCCGCAGT AATTCCTGGG
1201CAGAGATAGC ACCCATGAAA AACTGCCGGG AGCATTTTGT GCTGGGTGCC ATGGAGGAAT
1261ACCTCTATGC AGTTGGGGGC AGAAATGAAC TGCGCCAGGT TCTGCCTACA GTTGAGCGAT
1321ATTGCCCCAA GAAGAACAAA TGGACTTTTG TTCAGTCCTT TGACAGATCC CTTTCATGCC
1381ATGCTGGATA TGTGGCTGAT GGTCTTCTTT GGATATCAGG TAGAACATAC CTCATGTTGG
1441ATTTATCAAA ACACACTTTC ATTGTGGTAT ATATTTACCC AACTTTCTTG TACAAAGTGG
1501TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA
1561CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG
1621AGTTTAGTAA CCGAACCCGC TACATACGCG TTAAGTCgac aatcaacctc tggattacaa
1681aatttgtgaa agatt

Download FASTA (ORF) (Full)