Transcript: Human NM_001083620.1

Homo sapiens glutamate ionotropic receptor AMPA type subunit 2 (GRIA2), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-08-04
Taxon:
Homo sapiens (human)
Gene:
GRIA2 (2891)
Length:
5266
CDS:
112..2622

Additional Resources:

NCBI RefSeq record:
NM_001083620.1
NBCI Gene record:
GRIA2 (2891)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001083620.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000426386 TGCGGCAAGGATGCGATATTT pLKO_005 1787 CDS 100% 15.000 21.000 N GRIA2 n/a
2 TRCN0000102940 CGTGTTATGACTCCAGAATTT pLKO.1 2695 3UTR 100% 13.200 18.480 N Gria2 n/a
3 TRCN0000435950 GCAATTGCTCCATTAACTATT pLKO_005 1456 CDS 100% 13.200 18.480 N GRIA2 n/a
4 TRCN0000061685 CCCAGTAAATCTTGCAGTATT pLKO.1 2265 CDS 100% 13.200 10.560 N GRIA2 n/a
5 TRCN0000412709 GATGATTCGTTGGTATCTAAA pLKO_005 766 CDS 100% 13.200 10.560 N GRIA2 n/a
6 TRCN0000061684 CGCCAAACATTGTGGGTTCAA pLKO.1 1329 CDS 100% 4.950 3.960 N GRIA2 n/a
7 TRCN0000412632 TTACTGATGGAGACCTATTAA pLKO_005 695 CDS 100% 15.000 10.500 N GRIA2 n/a
8 TRCN0000061683 CCAGGTTATTACCATTGGAAA pLKO.1 633 CDS 100% 4.950 3.465 N GRIA2 n/a
9 TRCN0000061687 GCCGTTCAAGTGATGACTGAA pLKO.1 877 CDS 100% 4.950 3.465 N GRIA2 n/a
10 TRCN0000061686 GCTATCAATGTGGGAAACATT pLKO.1 499 CDS 100% 0.563 0.394 N GRIA2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001083620.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15434 pDONR223 0% 90.3% 90.3% None (many diffs) n/a
2 ccsbBroad304_15434 pLX_304 0% 90.3% 90.3% V5 (many diffs) n/a
3 TRCN0000466239 GCACCCCATCATGTAGGAATTACG pLX_317 14.1% 90.3% 90.3% V5 (many diffs) n/a
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