Transcript: Human NM_001100422.1

Homo sapiens spermatogenesis associated serine rich 2 like (SPATS2L), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-08-03
Taxon:
Homo sapiens (human)
Gene:
SPATS2L (26010)
Length:
6335
CDS:
413..2089

Additional Resources:

NCBI RefSeq record:
NM_001100422.1
NBCI Gene record:
SPATS2L (26010)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001100422.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000053101 CGGAGATTTAATCCACAGTAT pLKO.1 1715 CDS 100% 4.950 6.930 N SPATS2L n/a
2 TRCN0000290893 CGGAGATTTAATCCACAGTAT pLKO_005 1715 CDS 100% 4.950 6.930 N SPATS2L n/a
3 TRCN0000053100 GCACCGTTTCTCTAACTAGAT pLKO.1 1104 CDS 100% 4.950 6.930 N SPATS2L n/a
4 TRCN0000290823 GCACCGTTTCTCTAACTAGAT pLKO_005 1104 CDS 100% 4.950 6.930 N SPATS2L n/a
5 TRCN0000053098 CGGAGAAATTACACATCCAAA pLKO.1 1465 CDS 100% 4.950 3.960 N SPATS2L n/a
6 TRCN0000290894 CGGAGAAATTACACATCCAAA pLKO_005 1465 CDS 100% 4.950 3.960 N SPATS2L n/a
7 TRCN0000053099 CCACAGATTCTGCTAACGAAA pLKO.1 762 CDS 100% 4.950 3.465 N SPATS2L n/a
8 TRCN0000290892 CCACAGATTCTGCTAACGAAA pLKO_005 762 CDS 100% 4.950 3.465 N SPATS2L n/a
9 TRCN0000053102 GCTCGTCAGAAGAAAGCAGAA pLKO.1 1274 CDS 100% 4.050 2.835 N SPATS2L n/a
10 TRCN0000290891 GCTCGTCAGAAGAAAGCAGAA pLKO_005 1274 CDS 100% 4.050 2.835 N SPATS2L n/a
11 TRCN0000282603 GTTCCGTGAAGAAGATCAAAG pLKO_005 1155 CDS 100% 10.800 6.480 N Spats2l n/a
12 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 5995 3UTR 100% 5.625 2.813 Y KLHL30 n/a
13 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 5995 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001100422.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11798 pDONR223 100% 87.4% 87.4% None 189_191delAAA;444_650del n/a
2 ccsbBroad304_11798 pLX_304 0% 87.4% 87.4% V5 189_191delAAA;444_650del n/a
3 TRCN0000471007 CAGACCAACGATTAGCTAACTTTT pLX_317 25% 87.4% 87.4% V5 189_191delAAA;444_650del n/a
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