Construct: ORF TRCN0000471007

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018124.1_s317c1
Derived from:
ccsbBroadEn_11798
DNA Barcode:
CAGACCAACGATTAGCTAACTTTT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SPATS2L (26010)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000471007
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 26010 SPATS2L spermatogenesis associated ... NM_001100424.1 99.7% 99.7% 189_191delAAA
2 human 26010 SPATS2L spermatogenesis associated ... XM_005246459.1 99.7% 99.7% 189_191delAAA
3 human 26010 SPATS2L spermatogenesis associated ... XM_017003785.2 99.7% 99.7% 189_191delAAA
4 human 26010 SPATS2L spermatogenesis associated ... XM_017003786.1 99.7% 99.7% 189_191delAAA
5 human 26010 SPATS2L spermatogenesis associated ... XM_017003787.1 99.7% 99.7% 189_191delAAA
6 human 26010 SPATS2L spermatogenesis associated ... XM_024452788.1 99.7% 99.7% 189_191delAAA
7 human 26010 SPATS2L spermatogenesis associated ... XM_017003784.2 94% 94% 1_90del;279_281delAAA
8 human 26010 SPATS2L spermatogenesis associated ... NM_001100422.1 87.4% 87.4% 189_191delAAA;444_650del
9 human 26010 SPATS2L spermatogenesis associated ... NM_001100423.2 87.4% 87.4% 189_191delAAA;444_650del
10 human 26010 SPATS2L spermatogenesis associated ... NM_001282735.1 87.4% 87.4% 189_191delAAA;444_650del
11 human 26010 SPATS2L spermatogenesis associated ... NM_015535.3 87.4% 87.4% 189_191delAAA;444_650del
12 human 26010 SPATS2L spermatogenesis associated ... XM_005246456.1 87.4% 87.4% 189_191delAAA;444_650del
13 human 26010 SPATS2L spermatogenesis associated ... XM_005246457.1 87.4% 87.4% 189_191delAAA;444_650del
14 human 26010 SPATS2L spermatogenesis associated ... XM_005246458.2 87.4% 87.4% 189_191delAAA;444_650del
15 human 26010 SPATS2L spermatogenesis associated ... XM_011510939.1 87.4% 87.4% 189_191delAAA;444_650del
16 human 26010 SPATS2L spermatogenesis associated ... XM_024452785.1 87.4% 87.4% 189_191delAAA;444_650del
17 human 26010 SPATS2L spermatogenesis associated ... XM_024452786.1 87.4% 87.4% 189_191delAAA;444_650del
18 human 26010 SPATS2L spermatogenesis associated ... XM_024452787.1 87.4% 87.4% 189_191delAAA;444_650del
19 human 26010 SPATS2L spermatogenesis associated ... NM_001282744.1 82.9% 82.9% 1_90del;279_281delAAA;534_740del
20 human 26010 SPATS2L spermatogenesis associated ... XM_011510938.1 82.9% 82.9% 1_90del;279_281delAAA;534_740del
21 human 26010 SPATS2L spermatogenesis associated ... NM_001282743.1 76.7% 76.7% 0_1ins180;9_11delAAA;264_470del
22 mouse 67198 Spats2l spermatogenesis associated,... XM_011238567.1 87.1% 89.5% (many diffs)
23 mouse 67198 Spats2l spermatogenesis associated,... XM_006496216.3 83.5% 85.8% (many diffs)
24 mouse 67198 Spats2l spermatogenesis associated,... XM_006496217.3 83.5% 85.8% (many diffs)
25 mouse 67198 Spats2l spermatogenesis associated,... NM_001164566.1 76.4% 78.4% (many diffs)
26 mouse 67198 Spats2l spermatogenesis associated,... XM_006496215.3 76.2% 78.3% (many diffs)
27 mouse 67198 Spats2l spermatogenesis associated,... XM_006496218.3 76.2% 78.3% (many diffs)
28 mouse 67198 Spats2l spermatogenesis associated,... XM_017322122.1 73.7% 75.7% (many diffs)
29 mouse 67198 Spats2l spermatogenesis associated,... XM_017322123.1 73.7% 75.7% (many diffs)
30 mouse 67198 Spats2l spermatogenesis associated,... NM_144882.4 73.6% 75.6% (many diffs)
31 mouse 67198 Spats2l spermatogenesis associated,... XM_006496210.3 73.6% 75.6% (many diffs)
32 mouse 67198 Spats2l spermatogenesis associated,... XM_006496211.3 73.6% 75.6% (many diffs)
33 mouse 67198 Spats2l spermatogenesis associated,... XM_006496212.3 73.6% 75.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1530
ORF length:
1464
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc tgaactcaat actcatgtga atgtcaagga aaagatctat gcagttagat
121cagttgttcc caacaaaagc aataatgaaa tagtcctggt gctccaacag tttgatttta
181atgtggataa agccgtgcaa gcctttgtgg atggcagtgc aattcaagtt ctaaaagaat
241ggaatatgac aggaaagaag aacaataaaa gaaaaagaag caagtccaag cagcatcaag
301gcaacaaaga tgctaaagac aaggtggaga ggcctgaggc agggcccctg cagccgcagc
361caccacagat tcaaaacggc cccatgaatg gctgcgagaa ggacagctcg tccacagatt
421ctgctaacga aaaaccagcc cttatccctc gtgagaaaaa gatctcgata cttgaggaac
481cttcaaaggc acttcgtggg gtcacaggcc caaatattga gaaatcagtg aaggatttgc
541aacgctgcac cgtttctcta actagatatc gcgtcatgat taaggaagaa gtggatagtt
601ccgtgaagaa gatcaaagct gcctttgctg aattacacaa ctgcatcatt gacaaagaag
661tttcattaat ggcagaaatg gataaagtta aagaagaagc catggaaatc ctgactgctc
721gtcagaagaa agcagaagaa ctaaagagac tcactgacct tgccagtcag atggcagaga
781tgcagctggc cgaactcagg gcagaaatta agcactttgt cagcgagcgt aaatatgacg
841aggagctcgg gaaagctgcc cggttttcct gtgacatcga acagctgaag gcccaaatca
901tgctctgcgg agaaattaca catccaaaga acaactattc ctcaagaact ccctgcagct
961ccctgctgcc tctgctgaat gcgcacgcag caacctctgg gaaacagagt aacttttccc
1021gaaaatcatc cactcacaat aagccctctg aaggcaaagc ggcaaacccc aaaatggtga
1081gcagtctccc cagcaccgcc gacccctctc accagaccat gccggccaac aagcagaatg
1141gatcttctaa ccaaagacgg agatttaaTC CACAGTATCA TAACAACAGG CTAAATGGGC
1201CTGCCAAGTC GCAGGGCAGT GGGAATGAAG CCGAGCCACT GGGAAAGGGC AACAGCCGCC
1261ACGAACACAG AAGACAGCCG CACAACGGCT TCCGGCCCAA AAACAAAGGC GGTGCCAAAA
1321ATCAAGAGGC TTCCTTGGGG ATGAAGACCC CCGAGGCCCC GGCCCATTCT GAAAAGCCCC
1381GGCGAAGGCA GCACGCTGCA GACACCTCGG AGGCCAGGCC CTTCCGGGGT AGTGTCGGTA
1441GGGTTTCACA GTGCAATCTC TGCCCCACGA GAATAGAAGT TTCCACAGAT GCAGCAGTTC
1501TCTCAGTCCC GGCTGTGACG TTGGTGGCCT GCCCAACTTT CTTGTACAAA GTGGTTGATA
1561TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC
1621CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACAGAC
1681CAACGATTAG CTAACTTTTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg
1741tgaaagatt

Download FASTA (ORF) (Full)