Construct: ORF TRCN0000471007
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018124.1_s317c1
- Derived from:
- ccsbBroadEn_11798
- DNA Barcode:
- CAGACCAACGATTAGCTAACTTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SPATS2L (26010)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471007
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26010 | SPATS2L | spermatogenesis associated ... | NM_001100424.1 | 99.7% | 99.7% | 189_191delAAA |
| 2 | human | 26010 | SPATS2L | spermatogenesis associated ... | XM_005246459.1 | 99.7% | 99.7% | 189_191delAAA |
| 3 | human | 26010 | SPATS2L | spermatogenesis associated ... | XM_017003785.2 | 99.7% | 99.7% | 189_191delAAA |
| 4 | human | 26010 | SPATS2L | spermatogenesis associated ... | XM_017003786.1 | 99.7% | 99.7% | 189_191delAAA |
| 5 | human | 26010 | SPATS2L | spermatogenesis associated ... | XM_017003787.1 | 99.7% | 99.7% | 189_191delAAA |
| 6 | human | 26010 | SPATS2L | spermatogenesis associated ... | XM_024452788.1 | 99.7% | 99.7% | 189_191delAAA |
| 7 | human | 26010 | SPATS2L | spermatogenesis associated ... | XM_017003784.2 | 94% | 94% | 1_90del;279_281delAAA |
| 8 | human | 26010 | SPATS2L | spermatogenesis associated ... | NM_001100422.1 | 87.4% | 87.4% | 189_191delAAA;444_650del |
| 9 | human | 26010 | SPATS2L | spermatogenesis associated ... | NM_001100423.2 | 87.4% | 87.4% | 189_191delAAA;444_650del |
| 10 | human | 26010 | SPATS2L | spermatogenesis associated ... | NM_001282735.1 | 87.4% | 87.4% | 189_191delAAA;444_650del |
| 11 | human | 26010 | SPATS2L | spermatogenesis associated ... | NM_015535.3 | 87.4% | 87.4% | 189_191delAAA;444_650del |
| 12 | human | 26010 | SPATS2L | spermatogenesis associated ... | XM_005246456.1 | 87.4% | 87.4% | 189_191delAAA;444_650del |
| 13 | human | 26010 | SPATS2L | spermatogenesis associated ... | XM_005246457.1 | 87.4% | 87.4% | 189_191delAAA;444_650del |
| 14 | human | 26010 | SPATS2L | spermatogenesis associated ... | XM_005246458.2 | 87.4% | 87.4% | 189_191delAAA;444_650del |
| 15 | human | 26010 | SPATS2L | spermatogenesis associated ... | XM_011510939.1 | 87.4% | 87.4% | 189_191delAAA;444_650del |
| 16 | human | 26010 | SPATS2L | spermatogenesis associated ... | XM_024452785.1 | 87.4% | 87.4% | 189_191delAAA;444_650del |
| 17 | human | 26010 | SPATS2L | spermatogenesis associated ... | XM_024452786.1 | 87.4% | 87.4% | 189_191delAAA;444_650del |
| 18 | human | 26010 | SPATS2L | spermatogenesis associated ... | XM_024452787.1 | 87.4% | 87.4% | 189_191delAAA;444_650del |
| 19 | human | 26010 | SPATS2L | spermatogenesis associated ... | NM_001282744.1 | 82.9% | 82.9% | 1_90del;279_281delAAA;534_740del |
| 20 | human | 26010 | SPATS2L | spermatogenesis associated ... | XM_011510938.1 | 82.9% | 82.9% | 1_90del;279_281delAAA;534_740del |
| 21 | human | 26010 | SPATS2L | spermatogenesis associated ... | NM_001282743.1 | 76.7% | 76.7% | 0_1ins180;9_11delAAA;264_470del |
| 22 | mouse | 67198 | Spats2l | spermatogenesis associated,... | XM_011238567.1 | 87.1% | 89.5% | (many diffs) |
| 23 | mouse | 67198 | Spats2l | spermatogenesis associated,... | XM_006496216.3 | 83.5% | 85.8% | (many diffs) |
| 24 | mouse | 67198 | Spats2l | spermatogenesis associated,... | XM_006496217.3 | 83.5% | 85.8% | (many diffs) |
| 25 | mouse | 67198 | Spats2l | spermatogenesis associated,... | NM_001164566.1 | 76.4% | 78.4% | (many diffs) |
| 26 | mouse | 67198 | Spats2l | spermatogenesis associated,... | XM_006496215.3 | 76.2% | 78.3% | (many diffs) |
| 27 | mouse | 67198 | Spats2l | spermatogenesis associated,... | XM_006496218.3 | 76.2% | 78.3% | (many diffs) |
| 28 | mouse | 67198 | Spats2l | spermatogenesis associated,... | XM_017322122.1 | 73.7% | 75.7% | (many diffs) |
| 29 | mouse | 67198 | Spats2l | spermatogenesis associated,... | XM_017322123.1 | 73.7% | 75.7% | (many diffs) |
| 30 | mouse | 67198 | Spats2l | spermatogenesis associated,... | NM_144882.4 | 73.6% | 75.6% | (many diffs) |
| 31 | mouse | 67198 | Spats2l | spermatogenesis associated,... | XM_006496210.3 | 73.6% | 75.6% | (many diffs) |
| 32 | mouse | 67198 | Spats2l | spermatogenesis associated,... | XM_006496211.3 | 73.6% | 75.6% | (many diffs) |
| 33 | mouse | 67198 | Spats2l | spermatogenesis associated,... | XM_006496212.3 | 73.6% | 75.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1530
- ORF length:
- 1464
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgaactcaat actcatgtga atgtcaagga aaagatctat gcagttagat 121 cagttgttcc caacaaaagc aataatgaaa tagtcctggt gctccaacag tttgatttta 181 atgtggataa agccgtgcaa gcctttgtgg atggcagtgc aattcaagtt ctaaaagaat 241 ggaatatgac aggaaagaag aacaataaaa gaaaaagaag caagtccaag cagcatcaag 301 gcaacaaaga tgctaaagac aaggtggaga ggcctgaggc agggcccctg cagccgcagc 361 caccacagat tcaaaacggc cccatgaatg gctgcgagaa ggacagctcg tccacagatt 421 ctgctaacga aaaaccagcc cttatccctc gtgagaaaaa gatctcgata cttgaggaac 481 cttcaaaggc acttcgtggg gtcacaggcc caaatattga gaaatcagtg aaggatttgc 541 aacgctgcac cgtttctcta actagatatc gcgtcatgat taaggaagaa gtggatagtt 601 ccgtgaagaa gatcaaagct gcctttgctg aattacacaa ctgcatcatt gacaaagaag 661 tttcattaat ggcagaaatg gataaagtta aagaagaagc catggaaatc ctgactgctc 721 gtcagaagaa agcagaagaa ctaaagagac tcactgacct tgccagtcag atggcagaga 781 tgcagctggc cgaactcagg gcagaaatta agcactttgt cagcgagcgt aaatatgacg 841 aggagctcgg gaaagctgcc cggttttcct gtgacatcga acagctgaag gcccaaatca 901 tgctctgcgg agaaattaca catccaaaga acaactattc ctcaagaact ccctgcagct 961 ccctgctgcc tctgctgaat gcgcacgcag caacctctgg gaaacagagt aacttttccc 1021 gaaaatcatc cactcacaat aagccctctg aaggcaaagc ggcaaacccc aaaatggtga 1081 gcagtctccc cagcaccgcc gacccctctc accagaccat gccggccaac aagcagaatg 1141 gatcttctaa ccaaagacgg agatttaaTC CACAGTATCA TAACAACAGG CTAAATGGGC 1201 CTGCCAAGTC GCAGGGCAGT GGGAATGAAG CCGAGCCACT GGGAAAGGGC AACAGCCGCC 1261 ACGAACACAG AAGACAGCCG CACAACGGCT TCCGGCCCAA AAACAAAGGC GGTGCCAAAA 1321 ATCAAGAGGC TTCCTTGGGG ATGAAGACCC CCGAGGCCCC GGCCCATTCT GAAAAGCCCC 1381 GGCGAAGGCA GCACGCTGCA GACACCTCGG AGGCCAGGCC CTTCCGGGGT AGTGTCGGTA 1441 GGGTTTCACA GTGCAATCTC TGCCCCACGA GAATAGAAGT TTCCACAGAT GCAGCAGTTC 1501 TCTCAGTCCC GGCTGTGACG TTGGTGGCCT GCCCAACTTT CTTGTACAAA GTGGTTGATA 1561 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1621 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACAGAC 1681 CAACGATTAG CTAACTTTTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1741 tgaaagatt