Transcript: Mouse NM_001109972.1

Mus musculus SEC61, gamma subunit (Sec61g), transcript variant 3, mRNA.

Source:
NCBI, updated 2017-05-20
Taxon:
Mus musculus (mouse)
Gene:
Sec61g (20335)
Length:
758
CDS:
392..598

Additional Resources:

NCBI RefSeq record:
NM_001109972.1
NBCI Gene record:
Sec61g (20335)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001109972.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000100517 ACTGATCCATATACCCATTAA pLKO.1 556 CDS 100% 13.200 6.600 Y Sec61g n/a
2 TRCN0000334191 ACTGATCCATATACCCATTAA pLKO_005 556 CDS 100% 13.200 6.600 Y Sec61g n/a
3 TRCN0000374443 ACAAACATGGATCAGGTAATG pLKO_005 386 5UTR 100% 10.800 5.400 Y Sec61g n/a
4 TRCN0000100519 AGGACTCAATTCGGCTGGTTA pLKO.1 438 CDS 100% 4.950 2.475 Y Sec61g n/a
5 TRCN0000334190 AGGACTCAATTCGGCTGGTTA pLKO_005 438 CDS 100% 4.950 2.475 Y Sec61g n/a
6 TRCN0000100518 CATGGGATTCATTGGCTTCTT pLKO.1 529 CDS 100% 4.950 2.475 Y Sec61g n/a
7 TRCN0000334257 CATGGGATTCATTGGCTTCTT pLKO_005 529 CDS 100% 4.950 2.475 Y Sec61g n/a
8 TRCN0000381569 GATGCACCAAACCCGACAGAA pLKO_005 462 CDS 100% 4.950 2.475 Y Sec61g n/a
9 TRCN0000100516 GCAGTTTGTAAAGGACTCAAT pLKO.1 427 CDS 100% 4.950 2.475 Y Sec61g n/a
10 TRCN0000100515 CCTTCTCATCATGGGACGAGT pLKO.1 601 3UTR 100% 2.640 1.320 Y Sec61g n/a
11 TRCN0000273514 ATTCCAGAAGATTGCCATGGC pLKO_005 487 CDS 100% 2.160 1.080 Y SEC61G n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001109972.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02776 pDONR223 100% 92.1% 100% None (many diffs) n/a
2 ccsbBroad304_02776 pLX_304 0% 92.1% 100% V5 (many diffs) n/a
3 TRCN0000466863 AATTTTGGCGTTCCAACTTCGCGC pLX_317 100% 92.1% 100% V5 (many diffs) n/a
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