Construct: ORF ccsbBroad304_02776
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012446.1_s304c1, BRDN0000403304
- Derived from:
- ccsbBroadEn_02776
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SEC61G (23480)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02776
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23480 | SEC61G | SEC61 translocon gamma subunit | NM_001012456.1 | 100% | 100% | |
| 2 | human | 23480 | SEC61G | SEC61 translocon gamma subunit | NM_014302.4 | 100% | 100% | |
| 3 | mouse | 102637269 | LOC102637269 | protein transport protein S... | XM_006496391.3 | 92.6% | 100% | (many diffs) |
| 4 | mouse | 20335 | Sec61g | SEC61, gamma subunit | NM_001109971.1 | 92.1% | 100% | (many diffs) |
| 5 | mouse | 20335 | Sec61g | SEC61, gamma subunit | NM_001109972.1 | 92.1% | 100% | (many diffs) |
| 6 | mouse | 100043039 | Gm4184 | predicted gene 4184 | XM_006514391.1 | 92.1% | 100% | (many diffs) |
| 7 | mouse | 100046078 | Gm15266 | predicted gene 15266 | XM_003085302.1 | 89.7% | 86.7% | (many diffs) |
| 8 | mouse | 102632470 | Gm30534 | predicted gene, 30534 | XM_006512956.2 | 89.7% | 86.7% | (many diffs) |
| 9 | mouse | 669751 | Gm17756 | predicted gene, 17756 | XR_001781605.1 | 87.5% | (many diffs) | |
| 10 | mouse | 102640296 | Gm12881 | predicted gene 12881 | XM_006503510.1 | 65.9% | 63.4% | (many diffs) |
| 11 | mouse | 20335 | Sec61g | SEC61, gamma subunit | NM_011343.3 | 62.6% | 68% | (many diffs) |
| 12 | mouse | 108167643 | LOC108167643 | protein transport protein S... | XR_001779123.1 | 8.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 270
- ORF length:
- 204
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga tcaggtaatg cagtttgttg agccaagtcg gcagtttgta aaggactcca 121 ttcggctggt taaaagatgc actaaacctg atagaaaaga attccagaag attgccatgg 181 caacagcaat aggatttgct ataatgggat tcattggctt ctttgtgaaa ttgatccata 241 ttcctattaa taacatcatt gttggtggct gcccaacttt cttgtacaaa gtggttggta 301 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc 361 ggtggcgcgt taagtcgaca atcaacctct ggatta