Construct: ORF TRCN0000466863
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012446.1_s317c1
- Derived from:
- ccsbBroadEn_02776
- DNA Barcode:
- AATTTTGGCGTTCCAACTTCGCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SEC61G (23480)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466863
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23480 | SEC61G | SEC61 translocon gamma subunit | NM_001012456.1 | 100% | 100% | |
| 2 | human | 23480 | SEC61G | SEC61 translocon gamma subunit | NM_014302.4 | 100% | 100% | |
| 3 | mouse | 102637269 | LOC102637269 | protein transport protein S... | XM_006496391.3 | 92.6% | 100% | (many diffs) |
| 4 | mouse | 20335 | Sec61g | SEC61, gamma subunit | NM_001109971.1 | 92.1% | 100% | (many diffs) |
| 5 | mouse | 20335 | Sec61g | SEC61, gamma subunit | NM_001109972.1 | 92.1% | 100% | (many diffs) |
| 6 | mouse | 100043039 | Gm4184 | predicted gene 4184 | XM_006514391.1 | 92.1% | 100% | (many diffs) |
| 7 | mouse | 100046078 | Gm15266 | predicted gene 15266 | XM_003085302.1 | 89.7% | 86.7% | (many diffs) |
| 8 | mouse | 102632470 | Gm30534 | predicted gene, 30534 | XM_006512956.2 | 89.7% | 86.7% | (many diffs) |
| 9 | mouse | 669751 | Gm17756 | predicted gene, 17756 | XR_001781605.1 | 87.5% | (many diffs) | |
| 10 | mouse | 102640296 | Gm12881 | predicted gene 12881 | XM_006503510.1 | 65.9% | 63.4% | (many diffs) |
| 11 | mouse | 20335 | Sec61g | SEC61, gamma subunit | NM_011343.3 | 62.6% | 68% | (many diffs) |
| 12 | mouse | 108167643 | LOC108167643 | protein transport protein S... | XR_001779123.1 | 8.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 243
- ORF end:
- 447
- ORF length:
- 204
- Sequence:
-
1 TACGTCGTCT TTAGGTTGGG GGGAGGGGTT TTATGCGATG GAGTTTCCCC ACACTGAGTG 61 GGTGGAGACT GAAGTTAGGC CAGCTTGGCA CTTGATGTAA TTCTCCTTGG AATTTGCCCT 121 TTTTGAGTTT GGATCTTGGT TCATTCTCAA GCCTCAGACA GTGGTTCAAA GTTTTTTTCT 181 TCCATTTCAG GTGTCGTGAG GCTAGCATCG ATTGATCAAC AAGTTTGTAC AAAAAAGTTG 241 GCATGGATCA GGTAATGCAG TTTGTTGAGC CAAGTCGGCA GTTTGTAAAG GACTCCATTC 301 GGCTGGTTAA AAGATGCACT AAACCTGATA GAAAAGAATT CCAGAAGATT GCCATGGCAA 361 CAGCAATAGG ATTTGCTATA ATGGGATTCA TTGGCTTCTT TGTGAAATTG ATCCATATTC 421 CTATTAATAA CATCATTGTT GGTGGCTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 481 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 541 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAAATTTTGG 601 CGTTCCAACT TCGCGCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 661 aagatt