Transcript: Human NM_001111067.4

Homo sapiens activin A receptor type 1 (ACVR1), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-09-15
Taxon:
Homo sapiens (human)
Gene:
ACVR1 (90)
Length:
3332
CDS:
718..2247

Additional Resources:

NCBI RefSeq record:
NM_001111067.4
NBCI Gene record:
ACVR1 (90)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001147781 ATTACACTGTTGGAGTGTGT pXPR_003 CGG 638 42% 6 0.8839 ACVR1 ACVR1 76758
2 BRDN0001145448 CTGGTGTAACAGGAACATCA pXPR_003 CGG 307 20% 4 0.5158 ACVR1 ACVR1 76757
3 BRDN0001144892 CCATGACTTCTCATCACGGG pXPR_003 AGG 718 47% 7 -0.2158 ACVR1 ACVR1 76759
Download CSV

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001111067.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000382239 TGATAATTCCCTCGACAAATT pLKO_005 2211 CDS 100% 13.200 18.480 N ACVR1 n/a
2 TRCN0000000441 GCAGAACGTATTTAGCCATTA pLKO.1 3100 3UTR 100% 10.800 15.120 N ACVR1 n/a
3 TRCN0000000442 CGGATGGTGAGCAATGGTATA pLKO.1 1966 CDS 100% 10.800 8.640 N ACVR1 n/a
4 TRCN0000315051 CGGATGGTGAGCAATGGTATA pLKO_005 1966 CDS 100% 10.800 8.640 N ACVR1 n/a
5 TRCN0000195081 CTGGTCTGTCTTTGGATAATA pLKO.1 2755 3UTR 100% 15.000 10.500 N ACVR1 n/a
6 TRCN0000194668 CTTGCACTGTTACTCTTAATT pLKO.1 2694 3UTR 100% 15.000 10.500 N ACVR1 n/a
7 TRCN0000195486 CTTGGAGGTTGGCCTCATTAT pLKO.1 1080 CDS 100% 13.200 9.240 N ACVR1 n/a
8 TRCN0000000443 GACAGCACTTTAGCAGATTTA pLKO.1 1246 CDS 100% 13.200 9.240 N ACVR1 n/a
9 TRCN0000315118 GACAGCACTTTAGCAGATTTA pLKO_005 1246 CDS 100% 13.200 9.240 N ACVR1 n/a
10 TRCN0000000445 GTGGATTGTTTCGATTCTTAT pLKO.1 1894 CDS 100% 13.200 9.240 N ACVR1 n/a
11 TRCN0000315050 GTGGATTGTTTCGATTCTTAT pLKO_005 1894 CDS 100% 13.200 9.240 N ACVR1 n/a
12 TRCN0000321754 TGGTTCTCAGACCCGACATTA pLKO_005 2098 CDS 100% 13.200 9.240 N Acvr1 n/a
13 TRCN0000321822 TTGTCCAGCTGGGACCTAATG pLKO_005 2290 3UTR 100% 10.800 7.560 N Acvr1 n/a
14 TRCN0000000444 CTAATGAAAGAATGCTGGTAT pLKO.1 2134 CDS 100% 4.950 3.465 N ACVR1 n/a
15 TRCN0000199872 GCCTGACTGGTTGTCAGAATG pLKO.1 2314 3UTR 100% 1.080 0.756 N ACVR1 n/a
16 TRCN0000315052 GCCTGACTGGTTGTCAGAATG pLKO_005 2314 3UTR 100% 1.080 0.756 N ACVR1 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001111067.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05767 pDONR223 100% 99.8% 100% None 270C>T;690G>A n/a
2 ccsbBroad304_05767 pLX_304 0% 99.8% 100% V5 270C>T;690G>A n/a
3 TRCN0000469327 ACCAAAGGCGGGTTTGGTAGCTTC pLX_317 27.6% 99.8% 100% V5 270C>T;690G>A n/a
4 ccsbBroadEn_14527 pDONR223 0% 99.8% 100% None 270C>T;690G>A n/a
5 ccsbBroad304_14527 pLX_304 0% 99.8% 100% V5 270C>T;690G>A n/a
6 TRCN0000474710 GATATTCCTACCTCCATACTATTG pLX_317 30.3% 99.8% 100% V5 270C>T;690G>A n/a
Download CSV