Transcript: Mouse NM_001127264.1

Mus musculus transformation related protein 63 (Trp63), transcript variant 5, mRNA.

Source:
NCBI, updated 2017-06-18
Taxon:
Mus musculus (mouse)
Gene:
Trp63 (22061)
Length:
4707
CDS:
145..1893

Additional Resources:

NCBI RefSeq record:
NM_001127264.1
NBCI Gene record:
Trp63 (22061)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001127264.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000423330 GAATGAACAGACGTCCAATTT pLKO_005 806 CDS 100% 13.200 18.480 N Trp63 n/a
2 TRCN0000416977 TGATCGATGCCGTGCGCTTTA pLKO_005 1763 CDS 100% 10.800 15.120 N Trp63 n/a
3 TRCN0000012751 CGAGTGGAATGATTTCAACTT pLKO.1 1818 CDS 100% 4.950 6.930 N Trp63 n/a
4 TRCN0000012752 CCCAGTCATCTGATTCGAGTA pLKO.1 643 CDS 100% 4.050 5.670 N Trp63 n/a
5 TRCN0000012749 CCGTCAGAATACACACGGAAT pLKO.1 984 CDS 100% 4.050 5.670 N Trp63 n/a
6 TRCN0000430674 AGATGTTGCTGAAGATCAAAG pLKO_005 1085 CDS 100% 10.800 7.560 N Trp63 n/a
7 TRCN0000430928 AGTCAGCCACCTGGACGTATT pLKO_005 425 CDS 100% 10.800 7.560 N Trp63 n/a
8 TRCN0000012750 CCCAGTATGTAGAAGATCCTA pLKO.1 680 CDS 100% 3.000 2.100 N Trp63 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001127264.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01971 pDONR223 100% 77.6% 82% None (many diffs) n/a
2 ccsbBroad304_01971 pLX_304 0% 77.6% 82% V5 (many diffs) n/a
3 TRCN0000470450 GTGCTGAACACCGTCGATATCCGG pLX_317 21.6% 77.6% 82% V5 (many diffs) n/a
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