Transcript: Human NM_001130928.2

Homo sapiens myocyte enhancer factor 2A (MEF2A), transcript variant 4, mRNA.

Source:
NCBI, updated 2019-09-16
Taxon:
Homo sapiens (human)
Gene:
MEF2A (4205)
Length:
5167
CDS:
143..1432

Additional Resources:

NCBI RefSeq record:
NM_001130928.2
NBCI Gene record:
MEF2A (4205)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001130928.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000005132 GCCTAGAAATATAGAGCATTA pLKO.1 2118 3UTR 100% 10.800 15.120 N MEF2A n/a
2 TRCN0000431440 TTGGACTACTTGTTTCGTAAA pLKO_005 1707 3UTR 100% 10.800 15.120 N MEF2A n/a
3 TRCN0000418879 AGCTCTAGTAGCTCCTATGAT pLKO_005 1286 CDS 100% 5.625 7.875 N MEF2A n/a
4 TRCN0000005135 GCAGTTATCTCAGGGTTCCAA pLKO.1 1060 CDS 100% 3.000 2.400 N MEF2A n/a
5 TRCN0000432718 GCATCAAGTCCGAACCGATTT pLKO_005 1110 CDS 100% 10.800 7.560 N MEF2A n/a
6 TRCN0000005134 GCTTAGGCAAAGTCATGCCTA pLKO.1 669 CDS 100% 0.264 0.185 N MEF2A n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001130928.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10962 pDONR223 100% 97.2% 97.4% None 1_33del;657T>C;663G>A n/a
2 ccsbBroad304_10962 pLX_304 0% 97.2% 97.4% V5 1_33del;657T>C;663G>A n/a
3 TRCN0000479180 TCCACGCGCTGTGCATCGGTCAAG pLX_317 29.8% 97.2% 97.4% V5 1_33del;657T>C;663G>A n/a
4 ccsbBroadEn_06575 pDONR223 100% 83.7% 80.5% None (many diffs) n/a
5 ccsbBroad304_06575 pLX_304 0% 83.7% 80.5% V5 (many diffs) n/a
6 TRCN0000472816 CTCACACGAGAGGTCGTGTTGCCG pLX_317 9.8% 83.7% 80.5% V5 (many diffs) n/a
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