Construct: ORF TRCN0000472816

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003028.1_s317c1
Derived from:
ccsbBroadEn_06575
DNA Barcode:
CTCACACGAGAGGTCGTGTTGCCG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MEF2A (4205)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000472816
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4205 MEF2A myocyte enhancer factor 2A NM_001352617.2 99.8% 100% 873G>A;1494G>T
2 human 4205 MEF2A myocyte enhancer factor 2A NM_001365207.1 99.8% 100% 873G>A;1494G>T
3 human 4205 MEF2A myocyte enhancer factor 2A NM_005587.4 99.8% 100% 873G>A;1494G>T
4 human 4205 MEF2A myocyte enhancer factor 2A NM_001352618.2 99% 99.2% 396_407del;885G>A;1506G>T
5 human 4205 MEF2A myocyte enhancer factor 2A NM_001365206.1 99% 99.2% 396_407del;885G>A;1506G>T
6 human 4205 MEF2A myocyte enhancer factor 2A NM_001365203.1 98.2% 98.4% 865_888del;897G>A;1518G>T
7 human 4205 MEF2A myocyte enhancer factor 2A NM_001365204.1 98.2% 98.4% 865_888del;897G>A;1518G>T
8 human 4205 MEF2A myocyte enhancer factor 2A XM_011521582.2 98.2% 98.4% 865_888del;897G>A;1518G>T
9 human 4205 MEF2A myocyte enhancer factor 2A XM_017022191.1 98.2% 98.4% 865_888del;897G>A;1518G>T
10 human 4205 MEF2A myocyte enhancer factor 2A NM_001365201.1 97.5% 97.6% (many diffs)
11 human 4205 MEF2A myocyte enhancer factor 2A NM_001365202.1 97.5% 97.6% (many diffs)
12 human 4205 MEF2A myocyte enhancer factor 2A XM_011521572.2 97.5% 97.6% (many diffs)
13 human 4205 MEF2A myocyte enhancer factor 2A XM_011521573.2 97.5% 97.6% (many diffs)
14 human 4205 MEF2A myocyte enhancer factor 2A XM_011521577.2 97.5% 97.6% (many diffs)
15 human 4205 MEF2A myocyte enhancer factor 2A XM_011521578.2 97.5% 97.6% (many diffs)
16 human 4205 MEF2A myocyte enhancer factor 2A XM_011521579.2 97.5% 97.6% (many diffs)
17 human 4205 MEF2A myocyte enhancer factor 2A NM_001130926.2 94.8% 93.7% (many diffs)
18 human 4205 MEF2A myocyte enhancer factor 2A NM_001171894.3 94.8% 93.7% (many diffs)
19 human 4205 MEF2A myocyte enhancer factor 2A NM_001352614.2 94.8% 93.7% (many diffs)
20 human 4205 MEF2A myocyte enhancer factor 2A NM_001352615.2 94.8% 93.7% (many diffs)
21 human 4205 MEF2A myocyte enhancer factor 2A NM_001365208.1 94.8% 93.7% (many diffs)
22 human 4205 MEF2A myocyte enhancer factor 2A NM_001319206.2 93.3% 92.3% (many diffs)
23 human 4205 MEF2A myocyte enhancer factor 2A NM_001352616.2 93.3% 92.3% (many diffs)
24 human 4205 MEF2A myocyte enhancer factor 2A NM_001365205.1 93.3% 92.3% (many diffs)
25 human 4205 MEF2A myocyte enhancer factor 2A XM_017022195.1 86.2% 86.3% 52_53ins204;669G>A;1290G>T
26 human 4205 MEF2A myocyte enhancer factor 2A XM_011521585.2 84.2% 84.3% (many diffs)
27 human 4205 MEF2A myocyte enhancer factor 2A NM_001130928.2 83.7% 80.5% (many diffs)
28 human 4205 MEF2A myocyte enhancer factor 2A XM_011521587.2 83.7% 80.5% (many diffs)
29 human 4205 MEF2A myocyte enhancer factor 2A NM_001130927.3 82.4% 79.2% (many diffs)
30 human 4205 MEF2A myocyte enhancer factor 2A NM_001365209.1 82.4% 79.2% (many diffs)
31 human 4205 MEF2A myocyte enhancer factor 2A XM_017022200.1 75% 75.1% 0_1ins372;501G>A;1122G>T
32 human 4205 MEF2A myocyte enhancer factor 2A XM_017022201.1 75% 75.1% 0_1ins372;501G>A;1122G>T
33 human 4205 MEF2A myocyte enhancer factor 2A XM_017022199.1 74.4% 74.5% (many diffs)
34 human 4205 MEF2A myocyte enhancer factor 2A XM_017022197.2 73.8% 73.9% (many diffs)
35 human 4205 MEF2A myocyte enhancer factor 2A XM_017022198.2 73.8% 73.9% (many diffs)
36 human 4205 MEF2A myocyte enhancer factor 2A XM_017022196.1 73.2% 73.3% (many diffs)
37 human 4205 MEF2A myocyte enhancer factor 2A XM_017022202.1 70.4% 70.6% (many diffs)
38 human 4205 MEF2A myocyte enhancer factor 2A XM_011521590.2 58.8% 57.8% (many diffs)
39 human 4205 MEF2A myocyte enhancer factor 2A XR_001751289.1 52.6% (many diffs)
40 human 4205 MEF2A myocyte enhancer factor 2A NM_001365210.2 49% 46.4% (many diffs)
41 human 4205 MEF2A myocyte enhancer factor 2A XM_005254916.3 48.6% 46.1% (many diffs)
42 human 4205 MEF2A myocyte enhancer factor 2A NM_001365211.2 44.6% 40.2% (many diffs)
43 mouse 17258 Mef2a myocyte enhancer factor 2A NM_001291191.1 90% 95.5% (many diffs)
44 mouse 17258 Mef2a myocyte enhancer factor 2A NM_001291192.1 90% 95.5% (many diffs)
45 mouse 17258 Mef2a myocyte enhancer factor 2A XM_011250814.2 90% 95.5% (many diffs)
46 mouse 17258 Mef2a myocyte enhancer factor 2A XM_006540684.3 88.6% 94% (many diffs)
47 mouse 17258 Mef2a myocyte enhancer factor 2A XM_006540685.3 88.6% 94% (many diffs)
48 mouse 17258 Mef2a myocyte enhancer factor 2A NM_001291195.1 85% 89.3% (many diffs)
49 mouse 17258 Mef2a myocyte enhancer factor 2A NM_001033713.2 83.7% 87.9% (many diffs)
50 mouse 17258 Mef2a myocyte enhancer factor 2A XM_006540686.3 83.7% 87.9% (many diffs)
51 mouse 17258 Mef2a myocyte enhancer factor 2A XM_017322011.1 83.7% 87.9% (many diffs)
52 mouse 17258 Mef2a myocyte enhancer factor 2A NM_001291196.1 63.4% 67.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1563
ORF length:
1497
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggg gcggaagaaa atacaaatca cacgcataat ggatgaaagg aaccgacagg
121tcacttttac aaagagaaag tttggattaa tgaagaaagc ctatgaactt agtgtgctct
181gtgactgtga aatagcactc atcattttca acagctctaa caaactgttt caatatgcta
241gcactgatat ggacaaagtt cttctcaagt atacagaata taatgaacct catgaaagca
301gaaccaactc ggatattgtt gaggctctga acaagaagga acacagaggg tgcgacagcc
361cagaccctga tacttcatat gtgctaactc cacatacaga agaaaaatat aaaaaaatta
421atgaggaatt tgataatatg atgcggaatc ataaaatcgc acctggtctg ccacctcaga
481acttttcaat gtctgtcaca gttccagtga ccagccccaa tgctttgtcc tacactaacc
541cagggagttc actggtgtcc ccatctttgg cagccagctc aacgttaaca gattcaagca
601tgctctctcc acctcaaacc acattacata gaaatgtgtc tcctggagct cctcagagac
661caccaagtac tggcaatgca ggtgggatgt tgagcactac agacctcaca gtgccaaatg
721gagctggaag cagtccagtg gggaatggat ttgtaaactc aagagcttct ccaaatttga
781ttggagctac tggtgcaaat agcttaggca aagtcatgcc tacaaagtct ccccctccac
841caggtggtgg taatcttgga atgaacagta ggaaaccaga tcttcgagtt gtcatccccc
901cttcaagcaa gggcatgatg cctccactaa atacccaaag gatcagtagt tctcaagcca
961ctcaacctct tgctacccca gtcgtgtctg tgacaacccc aagcttgcct ccgcaaggac
1021ttgtgtactc agcaatgccg actgcctaca acactgatta ttcactgacc agcgctgacc
1081tgtcagccct tcaaggcttc aactcgccag gaatgctgtc gctgggacag gtgtcggcct
1141ggcagcagca ccacctagga caagcagccc tcagctctct tgttgctgga gggcagttat
1201ctcagggttc caatttatcc attaatacca accaaaacat cagcatcaag tccgaaccga
1261tttcacctcc tcgggatcgt atgaccccat cgggcttcca gcagcagcag cagcagcagc
1321agcagcagca gccgccgcca ccaccgcagc cccagccaca acccccgcag ccccagcccc
1381gacaggaaat ggggcgctcc cctgtggaca gtctgagcag ctcTAGTAGC TCCTATGATG
1441GCAGTGATCG GGAGGATCCA CGGGGCGACT TCCATTCTCC AATTGTGCTT GGCCGACCCC
1501CAAACACTGA GGACAGAGAA AGCCCTTCTG TAAAGCGAAT GAGGATGGAC GCGTGGGTTA
1561CCTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC
1621TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT
1681GGCTTTATAT ATCTTGTGGA AAGGACGACT CACACGAGAG GTCGTGTTGC CGACGCGTTA
1741AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt

Download FASTA (ORF) (Full)