Transcript: Human NM_001136219.2

Homo sapiens Fc fragment of IgG receptor IIa (FCGR2A), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-26
Taxon:
Homo sapiens (human)
Gene:
FCGR2A (2212)
Length:
2387
CDS:
12..965

Additional Resources:

NCBI RefSeq record:
NM_001136219.2
NBCI Gene record:
FCGR2A (2212)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001136219.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000436323 ACCTGTGGCTGCTTCAACCAT pLKO_005 55 CDS 100% 3.000 2.100 N FCGR2A n/a
2 TRCN0000436907 TGACTGTGCTTTCCGAATGGC pLKO_005 361 CDS 100% 2.640 1.848 N FCGR2A n/a
3 TRCN0000414699 CATGAATTGCGCCTCAGATTT pLKO_005 1402 3UTR 100% 13.200 6.600 Y FCGR2A n/a
4 TRCN0000029581 CCACAGTCACAGTGGTGATTA pLKO.1 545 CDS 100% 13.200 6.600 Y FCGR2B n/a
5 TRCN0000029575 GCACCTACTGACGATGATAAA pLKO.1 894 CDS 100% 13.200 6.600 Y FCGR2A n/a
6 TRCN0000029577 CCATGTCAACAGTAATAACTA pLKO.1 944 CDS 100% 5.625 2.813 Y FCGR2A n/a
7 TRCN0000029578 GAAGAAACCAACAATGACTAT pLKO.1 834 CDS 100% 4.950 2.475 Y FCGR2A n/a
8 TRCN0000029574 GCCATCAGAAAGAGACAACTT pLKO.1 813 CDS 100% 4.950 2.475 Y FCGR2A n/a
9 TRCN0000029584 GCCTTGATCTACTGCAGGAAA pLKO.1 720 CDS 100% 4.950 2.475 Y FCGR2C n/a
10 TRCN0000029587 CAGTGGTTCCACAATGGGAAT pLKO.1 231 CDS 100% 4.050 2.025 Y FCGR2C n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001136219.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06198 pDONR223 100% 99.5% 99.3% None 106_108delGCA;500A>G n/a
2 ccsbBroad304_06198 pLX_304 0% 99.5% 99.3% V5 106_108delGCA;500A>G n/a
3 TRCN0000472330 ACTGGGGCTCGAGCAGACAGCCGC pLX_317 46.5% 99.5% 99.3% V5 106_108delGCA;500A>G n/a
4 ccsbBroadEn_06199 pDONR223 100% 99.5% 99.3% None 106_108delGCA;652A>G n/a
5 ccsbBroad304_06199 pLX_304 0% 99.5% 99.3% V5 106_108delGCA;652A>G n/a
6 TRCN0000475216 TGCCCATTGCGGCCGTTTAATATC pLX_317 25% 99.5% 99.3% V5 106_108delGCA;652A>G n/a
7 ccsbBroadEn_00546 pDONR223 100% 77% 60.9% None (many diffs) n/a
8 ccsbBroad304_00546 pLX_304 0% 77% 60.9% V5 (many diffs) n/a
9 TRCN0000471042 AAGTCCACGAAAGCGTGGAATATT pLX_317 42.2% 77% 60.9% V5 (many diffs) n/a
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