Construct: ORF TRCN0000471042
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010933.1_s317c1
- Derived from:
- ccsbBroadEn_00546
- DNA Barcode:
- AAGTCCACGAAAGCGTGGAATATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FCGR2B (2213)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471042
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2213 | FCGR2B | Fc fragment of IgG receptor... | NM_004001.4 | 100% | 100% | |
| 2 | human | 2213 | FCGR2B | Fc fragment of IgG receptor... | NM_001002275.2 | 99.6% | 99.6% | 132_133insGCA |
| 3 | human | 2213 | FCGR2B | Fc fragment of IgG receptor... | XM_024454044.1 | 99.6% | 99.6% | 132_133insGCA |
| 4 | human | 2213 | FCGR2B | Fc fragment of IgG receptor... | NM_001190828.1 | 97.7% | 97.7% | 111_112ins21 |
| 5 | human | 2213 | FCGR2B | Fc fragment of IgG receptor... | NM_001002274.2 | 93.8% | 93.8% | 757_758ins57 |
| 6 | human | 2213 | FCGR2B | Fc fragment of IgG receptor... | NM_001002273.2 | 93.5% | 93.5% | 132_133insGCA;754_755ins57 |
| 7 | human | 2213 | FCGR2B | Fc fragment of IgG receptor... | XM_024454045.1 | 93.5% | 93.5% | 132_133insGCA;754_755ins57 |
| 8 | human | 9103 | FCGR2C | Fc fragment of IgG receptor... | NM_201563.5 | 87.9% | 79.1% | (many diffs) |
| 9 | human | 2213 | FCGR2B | Fc fragment of IgG receptor... | XM_024454043.1 | 84.8% | 85.6% | (many diffs) |
| 10 | human | 2213 | FCGR2B | Fc fragment of IgG receptor... | XM_017000670.2 | 84.5% | 85.3% | (many diffs) |
| 11 | human | 2212 | FCGR2A | Fc fragment of IgG receptor... | NM_021642.4 | 77% | 61% | (many diffs) |
| 12 | human | 2212 | FCGR2A | Fc fragment of IgG receptor... | NM_001136219.2 | 77% | 60.9% | (many diffs) |
| 13 | human | 2212 | FCGR2A | Fc fragment of IgG receptor... | XM_011509290.2 | 76.9% | 63.5% | (many diffs) |
| 14 | human | 2212 | FCGR2A | Fc fragment of IgG receptor... | XM_011509291.1 | 73.3% | 64.7% | (many diffs) |
| 15 | human | 2213 | FCGR2B | Fc fragment of IgG receptor... | XM_024454047.1 | 72.9% | 69.4% | (many diffs) |
| 16 | human | 2212 | FCGR2A | Fc fragment of IgG receptor... | XM_017000664.1 | 71.5% | 57.3% | (many diffs) |
| 17 | human | 2212 | FCGR2A | Fc fragment of IgG receptor... | XM_017000665.1 | 71.5% | 57.3% | (many diffs) |
| 18 | human | 2212 | FCGR2A | Fc fragment of IgG receptor... | XM_017000668.2 | 71.2% | 57.7% | (many diffs) |
| 19 | human | 2212 | FCGR2A | Fc fragment of IgG receptor... | XM_017000667.1 | 71.2% | 57.5% | (many diffs) |
| 20 | human | 2212 | FCGR2A | Fc fragment of IgG receptor... | XM_017000663.2 | 67.2% | 55.1% | (many diffs) |
| 21 | human | 2212 | FCGR2A | Fc fragment of IgG receptor... | XM_011509287.2 | 67.2% | 55% | (many diffs) |
| 22 | human | 2212 | FCGR2A | Fc fragment of IgG receptor... | XR_001737042.1 | 48.4% | (many diffs) | |
| 23 | human | 2213 | FCGR2B | Fc fragment of IgG receptor... | XR_002959731.1 | 40.9% | 1_91del;478_615del;704_705ins455 | |
| 24 | human | 2212 | FCGR2A | Fc fragment of IgG receptor... | XM_024454040.1 | 36.3% | 28.1% | (many diffs) |
| 25 | human | 9103 | FCGR2C | Fc fragment of IgG receptor... | NR_047648.1 | 34.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 996
- ORF length:
- 930
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg aatcctgtca ttcttacctg tccttgccac tgagagtgac tgggctgact 121 gcaagtcccc ccagccttgg ggtcatatgc ttctgtggac agctgtgcta ttcctggctc 181 ctgttgctgg gacacctgca gctcccccaa aggctgtgct gaaactcgag ccccagtgga 241 tcaacgtgct ccaggaggac tctgtgactc tgacatgccg ggggactcac agccctgaga 301 gcgactccat tcagtggttc cacaatggga atctcattcc cacccacacg cagcccagct 361 acaggttcaa ggccaacaac aatgacagcg gggagtacac gtgccagact ggccagacca 421 gcctcagcga ccctgtgcat ctgactgtgc tttctgagtg gctggtgctc cagacccctc 481 acctggagtt ccaggaggga gaaaccatcg tgctgaggtg ccacagctgg aaggacaagc 541 ctctggtcaa ggtcacattc ttccagaatg gaaaatccaa gaaattttcc cgttcggatc 601 ccaacTTCTC CATCCCACAA GCAAACCACA GTCACAGTGG TGATTACCAC TGCACAGGAA 661 ACATAGGCTA CACGCTGTAC TCATCCAAGC CTGTGACCAT CACTGTCCAA GCTCCCAGCT 721 CTTCACCGAT GGGGATCATT GTGGCTGTGG TCACTGGGAT TGCTGTAGCG GCCATTGTTG 781 CTGCTGTAGT GGCCTTGATC TACTGCAGGA AAAAGCGGAT TTCAGCTCTC CCAGGATACC 841 CTGAGTGCAG GGAAATGGGA GAGACCCTCC CTGAGAAACC AGCCAATCCC ACTAATCCTG 901 ATGAGGCTGA CAAAGTTGGG GCTGAGAACA CAATCACCTA TTCACTTCTC ATGCACCCGG 961 ATGCTCTGGA AGAGCCTGAT GACCAGAACC GTATTTACCC AACTTTCTTG TACAAAGTGG 1021 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1081 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1141 AAAGTCCACG AAAGCGTGGA ATATTACGCG TTAAGTCgac aatcaacctc tggattacaa 1201 aatttgtgaa agatt