Construct: ORF TRCN0000475216

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014812.1_s317c1
Derived from:
ccsbBroadEn_06199
DNA Barcode:
TGCCCATTGCGGCCGTTTAATATC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
FCGR2A (2212)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000475216
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2212 FCGR2A Fc fragment of IgG receptor... NM_021642.4 99.8% 99.6% 649A>G
2 human 2212 FCGR2A Fc fragment of IgG receptor... NM_001136219.2 99.5% 99.3% 106_108delGCA;652A>G
3 human 2212 FCGR2A Fc fragment of IgG receptor... XM_011509290.2 98.6% 98.4% (many diffs)
4 human 2212 FCGR2A Fc fragment of IgG receptor... XM_017000664.1 89.3% 88.3% (many diffs)
5 human 2212 FCGR2A Fc fragment of IgG receptor... XM_017000665.1 89.3% 88.3% (many diffs)
6 human 9103 FCGR2C Fc fragment of IgG receptor... NM_201563.5 88.1% 81.8% (many diffs)
7 human 2212 FCGR2A Fc fragment of IgG receptor... XM_017000668.2 87% 87% 614_615ins123
8 human 2212 FCGR2A Fc fragment of IgG receptor... XM_017000667.1 86.7% 86.7% 106_108delGCA;617_618ins123
9 human 2212 FCGR2A Fc fragment of IgG receptor... XM_017000663.2 85.5% 85% 649A>G;941_942delCAinsGT;948_1104delinsC
10 human 2212 FCGR2A Fc fragment of IgG receptor... XM_011509287.2 85.2% 84.8% (many diffs)
11 human 2212 FCGR2A Fc fragment of IgG receptor... XM_011509291.1 81.9% 78.5% (many diffs)
12 human 2213 FCGR2B Fc fragment of IgG receptor... NM_004001.4 76.9% 60.7% (many diffs)
13 human 2213 FCGR2B Fc fragment of IgG receptor... NM_001002275.2 76.8% 60.5% (many diffs)
14 human 2213 FCGR2B Fc fragment of IgG receptor... XM_024454044.1 76.8% 60.5% (many diffs)
15 human 2213 FCGR2B Fc fragment of IgG receptor... NM_001190828.1 76.6% 61% (many diffs)
16 human 2213 FCGR2B Fc fragment of IgG receptor... NM_001002274.2 74.9% 60.6% (many diffs)
17 human 2213 FCGR2B Fc fragment of IgG receptor... NM_001002273.2 74.8% 60.3% (many diffs)
18 human 2213 FCGR2B Fc fragment of IgG receptor... XM_024454045.1 74.8% 60.3% (many diffs)
19 human 2212 FCGR2A Fc fragment of IgG receptor... XM_017000666.1 74.2% 73.9% (many diffs)
20 human 2213 FCGR2B Fc fragment of IgG receptor... XM_024454043.1 71.7% 58.3% (many diffs)
21 human 2213 FCGR2B Fc fragment of IgG receptor... XM_017000670.2 71.6% 58% (many diffs)
22 human 2213 FCGR2B Fc fragment of IgG receptor... XM_024454047.1 60.2% 51.8% (many diffs)
23 human 2212 FCGR2A Fc fragment of IgG receptor... XR_001737042.1 58.4% (many diffs)
24 human 2212 FCGR2A Fc fragment of IgG receptor... XM_024454041.1 56.2% 56% 0_1ins414;235A>G
25 human 2212 FCGR2A Fc fragment of IgG receptor... XM_024454040.1 48% 47.5% (many diffs)
26 human 9103 FCGR2C Fc fragment of IgG receptor... NR_047648.1 34.7% (many diffs)
27 human 2213 FCGR2B Fc fragment of IgG receptor... XR_002959731.1 32.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1014
ORF length:
948
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgac tatggagacc caaatgtctc agaatgtatg tcccagaaac ctgtggctgc
121ttcaaccatt gacagttttg ctgctgctgg cttctgcaga cagtcaagct gctcccccaa
181aggctgtgct gaaacttgag cccccgtgga tcaacgtgct ccaggaggac tctgtgactc
241tgacatgcca gggggctcgc agccctgaga gcgactccat tcagtggttc cacaatggga
301atctcattcc cacccacacg cagcccagct acaggttcaa ggccaacaac aatgacagcg
361gggagtacac gtgccagact ggccagacca gcctcagcga ccctgtgcat ctgactgtgc
421tttccgaatg gctggtgctc cagacccctc acctggagtt ccaggaggga gaaaccatca
481tgctgaggtg ccacagctgg aaggacaagc ctctggtcaa ggtcacattc ttccagaatg
541gaaaatccca gaaattctcc catttggatc ccaccttctc catcccacaa gcaaaccaca
601gtcacagtgg tgattaccac tgcacaggaa acataggcta cacgctgttc tcatccaagc
661ctgtgaccat cactgtccaa gtgcccagca tgggcagctc ttcaccaatg ggggtcattg
721tggctgtggt cattgcgact gctgtagcag ccattgttgc tgctgtagtg gccttgatct
781acTGCAGGAA AAAGCGGATT TCAGCCAATT CCACTGATCC TGTGAAGGCT GCCCAATTTG
841AGCCACCTGG ACGTCAAATG ATTGCCATCA GAAAGAGACA ACTTGAAGAA ACCAACAATG
901ACTATGAAAC AGCTGACGGC GGCTACATGA CTCTGAACCC CAGGGCACCT ACTGACGATG
961ATAAAAACAT CTACCTGACT CTTCCTCCCA ACGACCATGT CAACAGTAAT AACTACCCAA
1021CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG
1081ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA
1141TATCTTGTGG AAAGGACGAT GCCCATTGCG GCCGTTTAAT ATCACGCGTT AAGTCgacaa
1201tcaacctctg gattacaaaa tttgtgaaag att

Download FASTA (ORF) (Full)