Transcript: Human NM_001141972.2

Homo sapiens diphthamine biosynthesis 6 (DPH6), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-05-31
Taxon:
Homo sapiens (human)
Gene:
DPH6 (89978)
Length:
3623
CDS:
25..522

Additional Resources:

NCBI RefSeq record:
NM_001141972.2
NBCI Gene record:
DPH6 (89978)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001141972.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000242719 CCCTCTATCGCCGAACCATAA pLKO_005 227 CDS 100% 10.800 15.120 N DPH6 n/a
2 TRCN0000242718 TATGAGTAGGTTGACATATTA pLKO_005 1606 3UTR 100% 15.000 12.000 N DPH6 n/a
3 TRCN0000242721 GGAAGGACAGCTGCTATAATA pLKO_005 53 CDS 100% 15.000 10.500 N DPH6 n/a
4 TRCN0000242720 ATGACCTTGCTTGCTGAATAT pLKO_005 349 CDS 100% 13.200 9.240 N DPH6 n/a
5 TRCN0000257161 AGATCGTTGCTTTAGCAAATC pLKO_005 101 CDS 100% 10.800 7.560 N DPH6 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001141972.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04502 pDONR223 100% 51.7% 44.1% None (many diffs) n/a
2 ccsbBroad304_04502 pLX_304 0% 51.7% 44.1% V5 (many diffs) n/a
3 TRCN0000467870 GACCCTCCCGTTTGTGAAGTCACG pLX_317 42.6% 51.7% 44.1% V5 (many diffs) n/a
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