Transcript: Human NM_001142679.2

Homo sapiens anoctamin 6 (ANO6), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-09-10
Taxon:
Homo sapiens (human)
Gene:
ANO6 (196527)
Length:
3483
CDS:
228..3017

Additional Resources:

NCBI RefSeq record:
NM_001142679.2
NBCI Gene record:
ANO6 (196527)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001142679.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000134143 CAGCGAAGAATTGACTTTGTT pLKO.1 423 CDS 100% 5.625 7.875 N ANO6 n/a
2 TRCN0000136872 CGATGTACTCACGTAGTGATA pLKO.1 1500 CDS 100% 4.950 6.930 N ANO6 n/a
3 TRCN0000134775 CCTTGGATCTTATCAGGAAAT pLKO.1 1075 CDS 100% 10.800 7.560 N ANO6 n/a
4 TRCN0000134710 GCTTCAGTTATTGGGATCATT pLKO.1 1629 CDS 100% 5.625 3.938 N ANO6 n/a
5 TRCN0000264189 CAAGTAGCTGGGACTACAGGA pLKO_005 3443 3UTR 100% 2.640 1.320 Y LINC01098 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001142679.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09792 pDONR223 100% 90.6% 87.3% None (many diffs) n/a
2 ccsbBroad304_09792 pLX_304 0% 90.6% 87.3% V5 (many diffs) n/a
3 TRCN0000477050 CAAAGAATCCAGTTCCGTGAGCGG pLX_317 15.4% 90.6% 87.3% V5 (many diffs) n/a
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